Adoption of veterinary vaccines, determining factors, and barriers in Southwest Ethiopia: Implications for livestock health and disease management strategies.

In Ethiopia, the use of veterinary vaccines to control animal diseases is an effective strategy. A study conducted in Southwest Ethiopia from October 2020 to October 2021 aimed to determine the adoption level of veterinary vaccines and factors affecting their use. The study used multistage random sampling to select districts and interviewed 476 farmers who had either adopted or not adopted the vaccines. The study found that certain diseases should be prioritized for vaccination to safeguard the health of cattle, sheep, goats, and poultry. These include anthrax (19.12 %), blackleg (17.65 %), foot and mouth disease (10.50 %), and lumpy skin disease (8.82 %) in cattle, and pasteurellosis (18.07 %), contagious caprine pleuropneumonia (15.97 %), peste des petits ruminants (14.15 %), and Orf (13.45 %) in sheep and goats. Newcastle disease (21.85 %), infectious bursal disease (19.33 %), and coccidiosis (17.02 %) were identified as high-priority diseases for flock health. Overall, 30.7 % of farmers were adopters of veterinary vaccines, while 69.3 % were non-adopters. The study identified several factors that influence the likelihood of adopting veterinary vaccines, including breed type (OR = 9.1, p < 0.0001), production size (OR = 9.7, p < 0.0001), production type (OR = 2.7, p < 0.0001), and farm location (OR = 9.8, p = 0.001). Common barriers to vaccination included a lack of disease knowledge, high vaccine costs, limited vaccine availability, and administration difficulties. Insights from the study can guide strategies for promoting veterinary vaccine adoption in Ethiopia. Stakeholders should pay attention to these findings since vaccine use is crucial for controlling animal diseases, enhancing animal health, and preventing economic losses. Further research is needed to investigate factors affecting enhanced veterinary vaccine adoption.

Seroprevalence of Coxiella burnetii, Leptospira interrogans serovar hardjo, and Brucella species and associated reproductive disorders in cattle in southwest Ethiopia.

In Ethiopia, Coxiella burnetii, Leptospira Hardjo, and Brucella spp are recognized as the primary factors contributing to cattle reproductive issues. A cross-sectional study was conducted in southwest Ethiopia from October 2020 to October 2021 to assess the risk of reproductive disorders associated with L. Hardjo, Coxiella burnetii, and Brucella spp. Moreover, the study aimed to identify the factors associated with reproductive disorders. Using an indirect ELISA, antibodies against these pathogens were observed in serum samples collected from 461 cattle. We employed multivariable random effect logistic regression analysis to identify potential risk factors associated with reproductive disorders in cattle. The study areas showed a prevalence of 25.16 % (95 % CI: 21.20-29.12) for cattle reproductive disorders. The presence of Leptospira Hardjo (OR = 2.9, 95 % CI: 1.17-4.02) and Coxiella burnetii (OR = 3.0, 1.49-5.94) antibodies was associated to the occurrence of cattle reproductive disorders. Seropositivity to pathogens B. abortus, C. burnetii, and L. Hardjo, along with co-infection of all three, showed association with cattle abortion. The presence of L. Hardjo seropositivity and co-infection with C. burnetii were related to dystocia in cattle. Cattle with retained fetal membranes were associated with co-infection seropositivity to these pathogens. Additionally, B. abortus seropositivity was linked to cases of repeated breeding in cattle. Age, breeding practices, and dog access to cattle showed associations with reproductive disorders, with odds ratios of 2.3 (95 % CI: 2.03-4.69), 2.9 (95 % CI: 1.83-4.82), and 6.5 (95 % CI: 1.04-2.53) respectively. This research indicates that Brucella abortus, Coxiella burnetii, and Leptospira Hardjo, which are responsible for severe zoonotic diseases, have a substantial negative impact on cattle production by causing reproductive disorders. To address the transmission of these diseases, it is essential to implement effective mitigation strategies and enhance public awareness. Additional investigation is necessary to identify and understand the factors contributing to cattle reproductive disorders in the specified area.

Using participatory epidemiology to investigate the causes of cattle abortion in Southwest Ethiopia.

Cattle abortion, stemming from both infectious and non-infectious causes, lead to notable financial setbacks in the cattle industry. Between October 2020 and October 2021, an epidemiological investigation took place in Southwest Ethiopia. The objective was to determine the magnitude and seasonal occurrence of the presumed causes of cattle abortion. Information for this research was collected through 30 group discussions, each involving 8-12 participants. Various participatory epidemiological tools, including semi-structured interviews, pairwise ranking, matrix scoring, proportional piling, and seasonal calendars, were employed in the designated areas. By employing the pairwise ranking approach, the relative significance of presumed causes contributing to cattle abortion was established. The identified major presumed causes of cattle abortion, listed in increasing order of importance, were blackleg, foot-and-mouth disease, pasteurellosis, lumpy skin disease, listeriosis, trypanosomosis, Q fever, leptospirosis, and brucellosis. Participants identified brucellosis (6.1%), leptospirosis (6.0%), and Q-fever (5.7%) as the primary presumed causes of abortion, determined through proportional piling. Matrix scoring analysis indicated a robust agreement (W = 0.464-0.989; P < 0.001) among different informant groups regarding both the presumed causes of abortion and the associated clinical signs. Brucellosis and Q-fever were perceived to be more prevalent during the dry season, while leptospirosis, listeriosis, and lumpy skin disease were associated with the wet, hot, and rainy seasons. However, Pasteurellosis, blackleg, and physical/mechanical factors were deemed to be consistently encountered causes of abortion throughout the year. The patterns of seasonal occurrence of suspected abortion causes were widely acknowledged across informant groups (W = 0.977-0.863; P < 0.001). Local practices involving herbal remedies and traditional methods were employed by participants to manage cattle abortion. Moreover, the results underscore the necessity for additional laboratory research to pinpoint the exact causes of abortion in the study areas.

Evaluation of livestock farmers' knowledge, attitudes and practices regarding the use of veterinary vaccines in Southwest Ethiopia.

BACKGROUND: The utilization of veterinary vaccines plays a vital role in preventing and managing animal diseases. However, the success of vaccination programmes relies on livestock farmers' understanding, perspectives and behaviours regarding their application. Therefore, this study aims to evaluate the knowledge, attitudes and practices of livestock farmers in Southwest Ethiopia regarding the use of veterinary vaccines. METHODS: The study followed a cross-sectional design conducted between October 2021 and October 2022. A sample of 476 livestock-owning farmers, including those who raise dairy cattle, beef cattle and poultry, were interviewed from the districts of Shei Bench, Semen Bench, Sheko and Yeki. RESULTS: The findings revealed that participants from Sheko, who had a poultry farm, were college graduates and resided in an urban area, were more likely to have good knowledge of veterinary vaccines. Similarly, participants who were male, from Semen Bench, had a poultry farm and had a high school education, exhibited a positive attitude towards veterinary vaccines. Furthermore, being male, from Semen Bench, having a poultry farm, and having a high school education were associated with increased veterinary vaccine use. CONCLUSION: This study highlights the importance of addressing gender differences and tailoring interventions based on geography and farming conditions in the area. These measures are crucial to improve practices related to veterinary vaccines for enhanced animal health and productivity. Prioritizing education, veterinary services and information dissemination is vital. However, further research and targeted interventions are needed to better comprehend underlying factors and implement effective strategies for diverse communities in different areas.

Epidemiological investigation of Coxiella burnetii in cattle and its association with Ixodid tick infestation in different agro-ecological zones of Southwest Ethiopia.

Coxiella burnetii is a serious zoonotic disease that causes significant economic losses in cattle production, including abortion, stillbirth, infertility, and reduced milk yield. However, little is known about the epidemiology of C. burnetii in Ethiopia. From November 2020 to November 2021, a cross-sectional study was conducted to estimate the seroprevalence and associated risk factors of C. burnetii in cattle in various agro-ecologies of Southwest Ethiopia. Blood samples were collected from 461 cattle, and the serum samples were tested for the presence of C. burnetii antibodies using an indirect ELISA. To identify potential risk factors for C. burnetii seropositivity, a multivariable mixed-effect logistic regression analysis was used. The study found an overall seroprevalence of 8.68% (95% CI: 6.11-11.25) and 13.57% (95% CI: 9.56-17.58) at the animal and herd levels, respectively, in the study areas. The results of the study indicated that C. burnetii infection was a widespread disease in the study areas. C. burnetii seropositivity at the animal level was significantly associated with age (OR = 4.1, 95%CI: 1.47-10.92), herd size (OR = 3.9, 95%CI: 1.21-12.66), management system (OR = 9.7, 95%CI: 1.27-27.25), cattle access to dogs, cats, and mice (OR = 2.5, 95%CI: 1.21-5.28), accessibility of cattle to wild animals (OR = 4.2, 95%CI: 1.01-17.18), presence of ticks on cattle (OR = 2.3, 95%CI: 1.12-4.83), and history of abortion (OR = 3.8, 95%CI: 1.78-8.23). A herd level analysis identified several risk factors for C. burnetii infection, including the management system (OR = 3.8, 95%CI: 1.59-8.98), agro-ecology (OR = 2.8, 95%CI: 1.43-7.21), herd size (OR = 4.3, 95%CI: 1.69-9.76), and accessibility of cattle to dogs, cats, and mice (OR = 2.6, 95%CI: 1.18-3.96). Therefore, it is important to implement appropriate control methods and raise public awareness about C. burnetii zoonotic transmission. Moreover, further studies should be conducted to isolate and characterize C. burnetii as a cause of reproductive problems and in disease reservoirs such as ticks and wildlife in the study areas.

Assessment of Humoral Immune Response in Pre- and Post-Vaccinated Cattle Against Lumpy Skin Disease.

Introduction: Lumpy skin disease (LSD) is viral disease affecting cattle production and productivity in Ethiopia. As a prevention method, vaccinations have been used for a long period with a questionable output due to the existence of LSD outbreaks in vaccinated herds in different parts of Ethiopia. Methods: A longitudinal study was performed from October 2019 to April 2020 with the objective of assessing the humoral immune response of cattle with a serum neutralization test (SNT) from different management systems in central Ethiopia. In this study, theserum was collected from 113 cattle (extensive (60/113) and intensive (53/113) management systems) before and after vaccination. Results and Discussion: From collected sera, a limited number of cattle had seroconversion before vaccination (7.08%). On the other hand, it is obvious the seroconversion rises post vaccination. Accordingly, seroconversion starts to increase after a week (8.85% at 7 dpv) post-vaccination which proceeds to significantly increase at 30 days post vaccination (dpv) (41.65% (25/60)). Furthermore, the risk factor study before and after vaccination showed intensively managed cattle with significantly higher levels of antibody titer at 7 dpv (OR = 1.17; 95% CI = 0.22, 6.2; p = 0.016) and 30 dpv (OR = 3.67; 95% CI = 1.1, 12.29; p = 0.035) compared with that of extensively managed cattle. The other animal-related risk factor that showed a significant difference was breeds and a specific age group ([4½, 7] years) at 15 dpv (OR = 6.69; 95% CI = 2.02, 22.08; p = 0.002) and 30 dpv (OR = 4.24; 95% CI = 1.22, 14.71; p = 0.023); respectively. Conclusion: This study showed an overall lower antibody detection across the study, posing a question on the current LSD-vaccine efficacy. Therefore, a circulating strain of LSDV should be cross-checked with the vaccine strain and adaptations should be made from it.

Herd and animal level seroprevalence and associated risk factors of bovine brucellosis in different agro-ecologies of southwest Ethiopia.

Brucellosis is a zoonotic infection that commonly affects cattle in Ethiopia, causing significant negative economic impact. A cross-sectional study was carried out between November 2020 and November 2021 in southwest Ethiopia to determine the seroprevalence of brucellosis and its associated risk factors in cattle herds. Blood samples were taken from 461 randomly selected cattle to test for the presence of Brucella antibodies using the Rose Bengal Plate test, with positive serum confirmed through the complement fixation test. A multivariable random effect logistic regression analysis was used to identify potential risk factors for Brucella seropositivity. The study found 7.14% (95% CI: 4.44-9.01) seroprevalence at the animal level and 12.23% (95% CI: 6.52-16.05) at the herd level based on the complement fixation test. Age (OR = 6.9, 95%CI: 1.83-15.97), herd size (OR = 3.66, 95%CI: 1.39-9.61), introducing new animals (OR = 2.72, 95%CI: 1.17-6.29), management system (OR = 12.2, 95%CI: 1.53-26.80), species composition (OR = 4.24, 95%CI: 1.51-11.91), and abortion (OR = 7.1, 95%CI: 1.93-15.39) were found to be associated with Brucella seropositivity. The analysis also revealed two risk factors for Brucella infection at the herd level, including herd size (OR = 3.4, 95% CI: 1.05-10.68) and species composition (OR = 3.1, 95% CI: 1.20-7.88). The presence of Brucella antibodies in cattle highlights the need for increased awareness and measures to mitigate the identified risk factors of the disease to prevent its spread. Furthermore, further studies are necessary to investigate the zoonotic transmission of brucellosis to humans and its role in cattle reproduction disorders in the study area.

Neglected zoonotic bacteria causes and associated risk factors of cattle abortion in different agro-ecological zones of southwest Ethiopia.

Three zoonotic bacteria, Coxiella burnetii, Leptospira spp, and Brucella spp, cause cattle abortion and significant losses in the livestock sector. However, these infections were neglected diseases in Ethiopia. Between October 2020 and October 2021, a cross-sectional study was carried out in southwest Ethiopia to determine the prevalence of abortion and identify the major zoonotic bacterial causes and risk factors that contribute to abortion. Using an indirect ELISA, antibodies to Brucella abortus, Leptospira Hardjo, and Coxiella burnetii were detected in serum samples taken from 461 cattle. Potential risk factors for cattle abortion were found using a multivariable random effect logistic regression analysis. The prevalence of cattle abortion was found to be 22.56 % (95 % CI: 18.74-26.38) in the study areas. Leptospira Hardjo (OR=1.8, 95% CI: 1.04-3.00), Coxiella burnetii (OR=2.7, 95 % CI: 1.26-5.62), and Brucella abortus (OR=9.8, 95 % CI: 1.27-17.15) were all associated with the occurrence of abortion in cattle, as were their co-infections (OR=8.1, 95 % CI: 1.28-39.33). Other risk factors for cattle abortion included breed (OR=16.7, 95 % CI: 2.20-26.77), herd size (OR=2.5, 95 % CI: 1.16-4.68), breeding method (OR=2.3, 95 % CI: 1.33-3.90), and contact with dogs (OR=2.2, 95 % CI: 1.34-3.53). According to the findings of this study, Brucella abortus, Coxiella burnetii, and Leptospira Hardjo are serious zoonoses that cause significant production loss in cattle due to abortion. To prevent transmission of these zoonotic diseases, it is crucial to implement effective control measures and increase public awareness. The causes of cattle abortion in the study areas should also be isolated and characterized.

Seroprevalence of Bovine Brucellosis in Selected Sites of Central Highland of Ethiopia.

Background: Brucellosis is a contagious, economically significant bacterial disease that affects animals worldwide and is one of the most neglected zoonotic diseases in the world. The disease poses a barrier to the trade of animals and animal products, represents a public health hazard, and is an impediment to free animal movement. Methods: A cross-sectional study was carried out from December 2019 to May 2020 in order to determine seroprevalence and identify potential risk factors for brucellosis in dairy cows in the Central Highlands of Ethiopia with recent cases of abortion. Purposive sampling was carried out on the farms and kebeles in question to screen for recent cases of abortion in dairy cows. For the purpose of performing serological testing, 352 blood samples from dairy cattle were obtained. The Rose Bengal Plate test was used to initially screen the serum samples, and the Complement Fixation test was utilized as a confirmatory test. Results: Using combined RBPT and CFT tests, the overall seroprevalence of bovine brucellosis was 0.6% (95% CI: 0.16-2.09). Retained fetal membrane (OR = 32.74, p = 0.006), market-based stock replacement (OR = 16.55, p = 0.002), breeding method (OR = 7.58, p = 0.027), and late stage of abortion (OR = 14.74, p = 0.0002) are all significantly associated risk factors. Conclusion: The present seroprevalence study revealed that brucellosis is prevalent at a lower rate among dairy cattle in the study areas. However, there is a possible risk of brucellosis transmission in dairy cattle and the exposed human population in research locations because no control measures were put in place there. Implementing a test and slaughter method with compensation for farmers is advised due to the low prevalence of bovine brucellosis in government-owned and small-holder farms.

Seroprevalence of human brucellosis in selected sites of Central Oromia, Ethiopia.

Brucellosis is one of the most neglected zoonotic diseases in the world. It affects all age groups and both sexes. A cross-sectional study was conducted from December 2019 to May 2020 to estimate the seroprevalence and assess the potential risk factors of brucellosis among dairy cow owners and dairy farmworkers, and assess their knowledge, attitudes, and practices in selected sites in the central highlands of Oromia, Ethiopia. A structured interview question was administered to 284 respondents, and only 166 of them volunteered to give a blood sample. Most respondents had limited knowledge of brucellosis (93.3%), zoonotic diseases transmitted by handling animal delivery (88%), and consuming raw milk and other animal products (90.0%). Accordingly, 149 blood samples from animal owners and 17 farmworkers were collected for serological testing. The serum samples collected were initially screened using the Rose Bengal Plate test, and the Complement Fixation test was used as a confirmatory test. The overall seroprevalence of zoonotic brucellosis was 1.2% (95%CI: 0.32-4.27). There was a statistically significant association of human brucellosis with human housing (OR = 1.8, p = 0.002), contact with aborted fetus (OR = 21.19, p = 0.017), drinking raw milk from non-aborted (OR = 24.99, p = 0.012), aborted (OR = 5.72, 0.019), and retained fetal membrane (OR = 4.22, p = 0.029) cows. In conclusion, the present study revealed that the seroprevalence of brucellosis in the study area was low. Public awareness among animal owners, farm and animal health workers on the transmission and health hazards of brucellosis needs to be addressed through community training. Implementing one health approach between veterinary and medical health professionals must be strengthened.