[Calcium, magnesium, copper and zinc in blood serum in men with femoral atherosclerosis]. / Wapn, magnez, miedz i cynk w surowicy krwi mezczyzn z miazdzyca tetnic udowych.

Calcium, magnesium, copper and zinc were determined in serum in men with femoral atherosclerosis. Also total cholesterol, HDL- and LDL-cholesterol were estimated. Subjects in control group were in the same range of age. The present study compares two methods of preparing serum for the flame atomic absorption spectrometry. In the first method (I), serum was diluted by the demineralized water. In the second method (II), serum protein was precipitated by trichloracetic acid (TCA). We found that the first method (I) was simpler and more precise than the other. The optimal proportions of serum and water amounted to 1 + 62 for calcium and magnesium and the coefficient of variation (C.V.) was 2.8%. For copper and zinc, the optimal dilution was 1 + 3 (C.V. = 1%). Total cholesterol was significantly higher (p < 0.05) and HDL-cholesterol was lower (p < 0.05) in subjects with femoral atherosclerosis as compared with controls. There was no difference in LDL-cholesterol. Subjects with femoral atherosclerosis had significantly higher level of all micro- and macroelements determined, then those of the control group (p < 0.05). However, higher serum copper does not necessary mean higher copper body status. It is possible that higher serum copper in femoral atherosclerosis reflects the transfer of copper from the tissue to the ceruloplasmin, as an acute phase reaction. On the other hand, the differences in serum magnesium, calcium, copper and zinc concentrations, may indicate the possible involvement of these elements in the disorder of total and HDL-cholesterol in femoral atherosclerosis.

[Lipid mobilizing activity in blood serum during the follicular and luteal phases of the menstrual cycle]. / Aktywnosc mobilizujaca lipidy surowicy krwi w fazie folikularnej i lutealnej cyklu miesiaczkowego.

The lipid mobilising activity of woman's blood serum in the follicular and luteal phases has been investigated. This activity was determined according to the Buckle method concerning parametrial adipose tissue of female rats. It was found to enhance the lipid mobilising activity in the follicular phase of the menstrual cycle. The increment of the lipid mobilising activity in the luteal phase was lower than in the follicular phase.

[Effect of progesterone on lipolysis in blood and adipose tissue of the female rat]. / Wplyw progesteronu na procesy lipolityczne we krwi i tkance tluszczowej samicy szczura.

The effect of progesterone on lipolysis in blood and in adipose tissue of female rats has been investigated. The hormone was administered to the studied animals subcutaneously during 20 days. The lipid mobilizing activity was determined in blood serum according to the four experimental protocols: test serum--test tissue, test serum--control tissue, control serum--test tissue, and control serum--control tissue. Blood serum concentrations of triglycerides, free fatty acids and glucose, as well as lipolytic activity against exogenous substrate, were determined in addition. Progesterone administration was found to enhance the activity of factors potentiating lipolysis in adipose tissue of female rats after 20 days administration of the hormone. There was an increase in free fatty acids, triglycerides and glucose concentrations. Progesterone was found not to change the activity of factors influencing lipolysis in the blood of female rats during the period of administration of the hormone.

[Effect of estradiol on lipolytic processes in the blood and adipose tissue of female rats]. / Wplyw estradiolu na procesy lipolityczne we krwi i tkance tluszczowej samicy szczura.

The effect of estradiol on lipolysis in blood and in adipose tissue of female rat has been investigated. The hormone was administered to the studied animals subcutaneously during 20 days. The lipid mobilizing activity was determined in blood serum according to the four different experimental protocols: test serum--test tissue, test serum--control tissue, control serum--test tissue, and control serum--control tissue. Blood serum concentrations of triglycerides, free fatty acids and glucose, as well as lipolytic activity against exogenous substrate, were determined in addition. Estradiol administration was found to enhance the activity of factors potentiating lipolysis both in the blood and in adipose tissue of female rats, inasmuch as an increase in free fatty acids and triglyceride concentration, and a decrease in glucose concentration were observed during the period of administration of the hormone.