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Electrophoresis ; 34(6): 854-61, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23335131

ABSTRACT

Dispersive liquid-liquid microextraction based on solidification of floating organic drop (DLLME-SFO) was for the first time combined with field-amplified sample injection (FASI) in CE to determine four ß(2)-agonists (cimbuterol, clenbuterol, mabuterol, and mapenterol) in bovine urine. Optimum BGE consisted of 20 mM borate buffer and 0.1 mM SDS. Using salting-out extraction, ß(2)-agonists were extracted into ACN that was then used as the disperser solvent in DLLME-SFO. Optimum DLLME-SFO conditions were: 1.0 mL ACN, 50 µL 1-undecanol (extraction solvent), total extraction time 1.5 min, no salt addition. Back extraction into an aqueous solution (pH 2.0) facilitated direct injection of ß(2)-agonists into CE. Compared to conventional CZE, DLLME-SFO-FASI-CE achieved sensitivity enhancement factors of 41-1046 resulting in LODs in the range of 1.80-37.0 µg L(-1). Linear dynamic ranges of 0.15-10.0 mg L(-1) for cimbuterol and 15-1000 µg L(-1) for the other analytes were obtained with coefficients of determination (R(2)) ≥ 0.9901 and RSD% ≤5.5 (n = 5). Finally, the applicability of the proposed method was successfully confirmed by determination of the four ß(2)-agonists in spiked bovine urine samples and accuracy higher than 96.0% was obtained.


Subject(s)
Adrenergic beta-2 Receptor Agonists/urine , Electrophoresis, Capillary/methods , Liquid Phase Microextraction/methods , 2-Hydroxyphenethylamine/analogs & derivatives , 2-Hydroxyphenethylamine/urine , Aniline Compounds/urine , Animals , Cattle , Clenbuterol/analogs & derivatives , Clenbuterol/urine , Hydrogen-Ion Concentration , Sensitivity and Specificity , Solvents
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