ABSTRACT
Male Wistar rats were maintained for 2 weeks on a semisynthetic ration with 0.4% rutin or on the same ration with 0.4% rutin and Lactobacillus casei 11,4001 suspension in physiological saline in a dose of 2 x 109 CFU per rat. Addition of Lactobacillus casei 114001 potentiated biological activity of rutin. Its antioxidant efficiency increased due to more pronounced increase in antioxidant capacity of the plasma, decrease in plasma content of LPO products, and more pronounced increase in reducing activity and antioxidant capacity of the cytosol of the liver and intestinal mucosa. The probiotic sharply increased the capacity of rutin to suppress pro carcinogenic activity of bacterial ß-glucuronidase.
Subject(s)
Antioxidants/pharmacology , Probiotics/pharmacology , Rutin/pharmacology , Animals , Carcinogens/antagonists & inhibitors , Drug Synergism , Glucuronidase/antagonists & inhibitors , Intestinal Mucosa/enzymology , Intestines/microbiology , Lacticaseibacillus casei , Liver , Male , Rats , Rats, Wistar , Rutin/metabolismABSTRACT
Antioxidant properties of 14 strains of lactic acid bacteria were evaluated in vitro using FRAP assay, inhibition of luminol oxidation in Hb-H2O2 system and inhibition of NADPH-Fe2+ induced microsomal lipid peroxidation. All strains demonstrated high reducing properties, but only L. casei spp. (including L. casei 114001) and L. fermentum ME-3 revealed pronounced ability to suppress oxidation of luminol (by 43-65,8%) and microsomal lipid peroxidation (by 57,9-89,5%). Either L. casei 114001 (10(8) CFU suspended in physiological solution) or fermented dairy drink containing equivalent amount of L. casei 114001 were daily administered orally to male Wistar rats. Antioxidant capacity of blood plasma, liver and intestines of animals elevated while MDA content in blood plasma decreased.
Subject(s)
Antioxidants/pharmacology , Lactobacillus/physiology , Probiotics/pharmacology , Yogurt/microbiology , Animals , Antioxidants/chemistry , Antioxidants/therapeutic use , Cytosol/enzymology , Cytosol/metabolism , In Vitro Techniques , Intestinal Mucosa/enzymology , Intestinal Mucosa/metabolism , Lactobacillus/growth & development , Lipid Peroxidation/drug effects , Lipid Peroxides/blood , Lipid Peroxides/metabolism , Male , Malondialdehyde/blood , Malondialdehyde/metabolism , Microsomes, Liver/drug effects , Microsomes, Liver/enzymology , Microsomes, Liver/metabolism , Oxidative Stress/drug effects , Probiotics/chemistry , Probiotics/therapeutic use , Rats , Rats, Wistar , Superoxide Dismutase/metabolismABSTRACT
Daily oral administration to rats of probiotic strain Lactobacillus casei 114001 (L.c.) at dose 2,8 x 10(10) cfu/rat during 8 days reduced oxidative stress and liver lesions, induced by a single intraperitoneal administration of carbon tetrachloride (CCl4) at dose 0.5 ml/kg b.w. It was evidenced by several histopathological and biochemical markers, characteristic for CCl4 toroxicity. Membrane damage by toxin was reduced in rats, treated with L.c.: alanine aminotransferase activity in plasma and nonsedimentable activity of lysosomal enzymes in liver were significantly decreased. Treatment with L.c. resulted in partial recovery of activities of antioxidant enzymes and enzymes of xenobiotic metabolism and full recovery of antioxidant capacity of liver cytosol. High level of activity and expression of proteins heme oxygenase and Nrf2 were maintained.
Subject(s)
Antioxidants , Carbon Tetrachloride Poisoning/prevention & control , Carbon Tetrachloride/pharmacology , Lacticaseibacillus casei , Alanine Transaminase/metabolism , Animals , Biomarkers/metabolism , Carbon Tetrachloride Poisoning/enzymology , Carbon Tetrachloride Poisoning/pathology , Cell Membrane/enzymology , Cell Membrane/pathology , Chemical and Drug Induced Liver Injury , Cytosol/enzymology , Cytosol/metabolism , Gene Expression Regulation, Enzymologic/drug effects , Heme Oxygenase (Decyclizing)/biosynthesis , Lysosomes/enzymology , Lysosomes/pathology , Male , NF-E2-Related Factor 2/biosynthesis , RatsABSTRACT
We have used the enhanced green fluorescent protein (EGFP) to investigate the properties of surfactant-entrapped water pools in organic solvents (reversed micelles) with steady-state and time-resolved fluorescence methods. The surfactant used was sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and the organic solvents were isooctane and (the more viscous) dodecane, respectively. The water content of the water pools could be controlled through the parameter w0, which is the water-to-surfactant molar ratio. With steady-state fluorescence, it was observed that subtle fluorescence changes could be noted in reversed micelles of different water contents. EGFP can be used as a pH-indicator of the water droplets in reversed micelles. Time-resolved fluorescence methods also revealed subtle changes in fluorescence decay times when the results in bulk water were compared with those in reversed micelles. The average fluorescence lifetimes of EGFP scaled with the relative fluorescence intensities. Time-resolved fluorescence anisotropy of EGFP in aqueous solution and reversed micelles yielded single rotational correlation times. Geometrical considerations could assign the observed correlation times to dehydrated protein at low w0 and internal EGFP rotation within the droplet at the highest w0.
