ABSTRACT
Acetylation phenotype was studied in 70 inpatients with acute viral hepatitis B. Of them, 51 had fast and 19 slow acetylation. Fast-type acetylation was associated with mild-severe form of hepatitis B, while slow-type acetylation--with recurrences and aggravations, severe form of hepatitis. The duration of HB antigenemia in the slow-type group was twice as long as in the fast-type group. Two patients, "slow acetylators", suffered from hepatitis B in the mild-severe form, later on chronic active hepatitis (CAH) developed. In "fast" acetylators the development of CAH was not observed. Thus, determination of phenotype by acetylation may be used for prognosis of acute viral hepatitis severity as well as for identification of high-risk groups and planning policy of follow-up.
Subject(s)
Hepatitis B/urine , Acetylation , Acute Disease , Adolescent , Adult , Hepatitis B/immunology , Hepatitis B Surface Antigens/blood , Humans , Middle Aged , Phenotype , Prognosis , Recurrence , Severity of Illness Index , Sulfathiazole , Sulfathiazoles/urineABSTRACT
The regulation of terephthalate catabolism was studied in Rhodococcus rubropertinctus which decomposed this synthetic monomer. The pathway (a) of terephthalate (TP) catabolism is as follows: TP----benzoate----4-hydroxybenzoate----protocatechuate----pyrocatechol-- --cycle ortho-cleavage. The following results were obtained when studying why two other catabolic pathways were realized if benzoate and 4-hydroxybenzoate were taken as a sole carbon source, namely, (b) benzoate----pyrocatechol----cycle cleavage and (c) 4-hydroxybenzoate----protocatechuate----cycle cleavage. TP seemed to cause the divergence of pathways (a) and (b) by repressing the system of benzoate oxidation to pyrocatechol. In pathway (c), benzoate repressed the synthesis of enzymes which catalysed protocatechuate oxidation. Pathway (b) was switched over to (a) when the strain was grown in a medium containing TP and benzoate at a benzoate concentration above 5 mM. Here, the concentration of benzoate (first exogenous and later formed from TP) played a key role. R. rubropertinctus growth in a medium with TP and glucose had diauxic characteristics.
Subject(s)
Phthalic Acids/metabolism , Rhodococcus/metabolism , Biodegradation, Environmental , Enzyme Induction , Kinetics , Oxidation-Reduction , Rhodococcus/enzymologyABSTRACT
A strain of Mycobacterium lacticolum destructing the isomeric aryldicarbonic acids: teraphthalic, isophthalic and phthalic (in decreasing degree) has been selected from bacterial community of terephthalate containing industrial sewage biotreatment. The dominating role of M. lacticolum in microbial community increases during the continuous-flow cultivation under condition of terephthalate concentration increasing. Minor members of microbial community are not able to oxidize terephthalate and utilize it in compound culture, but the community's capacity to attack high concentrations of this substance is much lesser than in case of M. lacticolum.
Subject(s)
Mycobacterium/enzymology , Phthalic Acids/metabolism , Biodegradation, Environmental , Industrial Waste , Isomerism , Mycobacterium/growth & developmentABSTRACT
A bacterial cenosis responsible for the destruction of organic wastes having a complex composition was selected under the conditions of continuous cultivation on highly concentrated industrial sewage. Most of the bacterial species and all of the yeasts and fungi were eliminated from the original microflora when the concentration of the purified sewage was increased from 2 to 12 g O2 per litre (in terms of the overall chemical oxygen uptake, COU) and the dilution rate (D) varied from 0.08 to 0.25 h-1. The number of species was reduced to 10 in the cenosis. The degree of oxidation of organic compounds in the sewage was 94 to 97% at the maximal parameters of the process. The specific rate of oxidation as well as the respiration and dehydrogenase activities increased with a rise in both COU and D. The highest biochemical activity was observed at D=0.25 h-1:
