ABSTRACT
African swine fever virus polypeptides p14 and p31 are synthesized in the presence of phosphonacetic acid which inhibits viral DNA replication, and therefore they are early viral proteins. These polypeptides were found to be localized on plasma membranes by immunofluorescence with monospecific antisera and monoclonal antibodies and by selective solubilization of infected cells. The p14-specific antibodies mediate complement-dependent cytolysis and antibody-dependent cytotoxicity of the cells infected with African swine fever virus.
Subject(s)
African Swine Fever Virus/chemistry , African Swine Fever/metabolism , Membrane Proteins/analysis , Peptides/analysis , Viral Proteins/analysis , African Swine Fever/immunology , African Swine Fever/microbiology , African Swine Fever Virus/immunology , Animals , Antibody-Dependent Cell Cytotoxicity , Cells, Cultured/immunology , Cells, Cultured/metabolism , Cells, Cultured/microbiology , Complement Hemolytic Activity Assay , Electrophoresis, Polyacrylamide Gel , Fluorescent Antibody Technique , Immunoblotting , Membrane Proteins/immunology , Membrane Proteins/isolation & purification , Molecular Weight , Peptides/immunology , Peptides/isolation & purification , Rabbits , Swine , Time Factors , Viral Proteins/immunology , Viral Proteins/isolation & purificationABSTRACT
The review deals with the methods of identification of virus-specific proteins on virion and infected cell surface. The isotopic labeling of membrane proteins, their extraction by proteolytic enzymes and selective solubilization by detergents are considered. The plasmatic membrane isolation by centrifugation and by means of microcarriers is described. The methods of membrane protein localization using monoclonal antibodies and radioimmunoprecipitation are presented.