ABSTRACT
The adult rats received both neurotoxin 6-hidroxidophamine and neurotoxin and melatonin. It was investigated a link between the disturbances of the brain antioxidant enzymes activity and thymic endocrine function, as possible pathogenic factors of parkinsonism, with changes in the number of neural stem cells (NSC) in the bulbus olfactorius. Rats with motor asymmetry in the apomorphine test and significant damage of the dopaminergic neurons in the-substantia nigra have decreased levels of superoxide dismutase, catalase and glutathione peroxidase activities in striatum (1.3-1.4 times) and blood thymulin content (8 times) compared to control group. On the contrary, examined indices were not changed in rats without motor asymmetry and correspondingly partly damaged neurons. The number of nestin(+)-cells in the bulbus olfactorius of rats without motor asymmetry increased from 91.2% to 99.3% and remained unchanged after melatonin administration course (10 mg/kg during 18 days). Melatonin administration resulted in the decrease in the number of nestin(+)-cells along with significant elevation of the decreased antioxidant enzymes activity and blood thymulin content in rats with circulatory movements. Possibilities of the enhancement of NSC differentiation in bulbus olfactorius into neuronal direction in such animals has been discussed. The conclusion about the potential use of melatonin as a neuroprotector in parkinsonism therapy has been made.
Subject(s)
Antioxidants/pharmacology , Ataxia/prevention & control , Melatonin/pharmacology , Neuroprotective Agents/pharmacology , Olfactory Bulb/drug effects , Parkinson Disease, Secondary/drug therapy , Thymus Gland/drug effects , Animals , Apomorphine/antagonists & inhibitors , Apomorphine/pharmacology , Ataxia/chemically induced , Ataxia/genetics , Ataxia/pathology , Catalase/genetics , Catalase/metabolism , Cell Count , Cell Differentiation/drug effects , Dopamine Agonists/pharmacology , Gene Expression , Glutathione Peroxidase/genetics , Glutathione Peroxidase/metabolism , Male , Nestin/genetics , Nestin/metabolism , Neural Stem Cells/cytology , Neural Stem Cells/drug effects , Neural Stem Cells/metabolism , Olfactory Bulb/metabolism , Olfactory Bulb/pathology , Oxidopamine , Parkinson Disease, Secondary/chemically induced , Parkinson Disease, Secondary/genetics , Parkinson Disease, Secondary/pathology , Rats , Rats, Wistar , Superoxide Dismutase/genetics , Superoxide Dismutase/metabolism , Thymic Factor, Circulating/genetics , Thymic Factor, Circulating/metabolism , Thymus Gland/metabolism , Thymus Gland/pathologyABSTRACT
Correlative relations of the liver supernatant enzymatic activity of catalase (CAT) and glutathione-peroxidase (GP) with the velocities of oxygen consumption (VO2) and carbon dioxide exhalation (VCO2) were analyzed by the two- and three-dimensional linear and non-linear statistical methods in mice. It is shown that despite the close functions, CAT and GP, nevertheless, exhibit opposite correlative and regressive links with the gaseous exchange indices. As was found by the pairwise linear method, the activity correlation with VO2 was positive for CAT, but negative for GP. The opposite patterns of correlation were also apparent under the three-dimensional non-linear analysis.
Subject(s)
Catalase/metabolism , Glutathione Peroxidase/metabolism , Liver/enzymology , Oxygen Consumption/physiology , Animals , Carbon Dioxide/metabolism , Data Interpretation, Statistical , Liver/metabolism , Mice , Mice, Inbred CBA , Oxygen/metabolismABSTRACT
Qualitative and quantitative differences in correlative and regressive links between superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase were assessed in the mice liver by two- and three-dimensional statistical methods. Paired linear correlation analysis indicated SOD-CAT tandem as the correlatively acting enzymatic pair. Three-dimensional analysis revealed uniform response surfaces which exhibited higher activities at disproportional values of the other two and lower activities at proportional activities of the other two enzymes. The direct effect of the enzymes on each other was positive [table: see text] while the effect of their product was always negative.
