1.
Lab Delo
; (2): 40-1, 1988.
Article
in Russian
| MEDLINE
| ID: mdl-2452287
2.
Ontogenez
; 15(5): 556-9, 1984.
Article
in Russian
| MEDLINE
| ID: mdl-6504502
ABSTRACT
To detect the hybrid cells forming as a result of fusion of the mouse myeloma cells with the immunic mouse splenocytes, a radioautographic method was used which involved the application of 3H-hypoxanthine as a labelled precursor of nucleic acids and of high-sensitive UK emulsion for accelerated preparations of autographs. The optimal conditions of hybridization providing for the maximum yield of hybrid cells and hybrid clones in our experiments were: use of polyethylene glycol ("Löba", mol. weight 4000) in 50% (w/v) concentration, of NS-O or X-653 myeloma cells in the ratio of parental cells 1:5 (myeloma cell: splenocyte).
Subject(s)
Autoradiography/methods , Hybridomas/cytology , Animals , Cell Fusion , Cell Line , Emulsions , Immunization , Mice , Multiple Myeloma/pathology , Spleen/cytology
3.
Lab Delo
; (7): 394-7, 1982.
Article
in Russian
| MEDLINE
| ID: mdl-6181298
4.
Tetrahedron
; 24(19): 6131-43, 1968 Oct.
Article
in English
| MEDLINE
| ID: mdl-5729561