Prevalent fecal contamination in drinking water resources and potential health risks in Swat, Pakistan.

Fecal bacteria contaminate water resources and result in associated waterborne diseases. This study assessed drinking water quality and evaluated their potential health risks in Swat, Pakistan. Ground and surface drinking water were randomly collected from upstream to downstream in the River Swat watershed and analyzed for fecal contamination using fecal indicator bacteria (Escherichia coli) and physiochemical parameters (potential of hydrogen, turbidity, temperature, electrical conductivity, total dissolved solid, color, odor and taste). The physiochemical parameters were within their safe limits except in a few locations, whereas, the fecal contaminations in drinking water resources exceeded the drinking water quality standards of Pakistan Environmental Protection Agency (Pak-EPA), 2008 and World Health Organization (WHO), 2011. Multivariate and univariate analyses revealed that downstream urbanization trend, minimum distance between water sources and pit latrines/sewerage systems, raw sewage deep well injection and amplified urban, pastures and agricultural runoffs having human and animal excreta were the possible sources of contamination. The questionnaire survey revealed that majority of the local people using 10-20years old drinking water supply schemes at the rate of 73% well supply, 13% hand pump supply, 11% spring supply and 3% river/streams supply, which spreads high prevalence of water borne diseases including hepatitis, intestinal infections and diarrhea, dysentery, cholera, typhoid fever, jaundice and skin diseases in children followed by older and younger adults.

Impacts of urbanization and landscape patterns on the earthworm communities in residential areas in Beijing.

Earthworms play an important role in soil processes and functions. However, few studies have focused on their community patterns in perturbed systems, especially in an urban environment with a high turnover rate of land cover. In this study, we collected and identified the earthworms in the residential areas in metropolitan Beijing. We further investigated the effects of urban soil properties, urbanization and landscape patterns on the earthworm communities. The results showed that both the abundance and biomass of earthworms in residential areas in metropolitan was relatively low. The abundance of earthworms was negatively correlated with soil organic carbon (SOC) in this study. Soil moisture and pH could be considered as the most important edaphic variables that affected earthworm communities. The construction age of residential areas significantly influenced the earthworm abundance. Moreover, the earthworm community composition responded differently to urban landscape features at different scales. The percentage of impervious and green space surface, the amount of landscape cover types, patch density and landscape fragment significantly affected the earthworm assemblages. Our result discovered that the edaphic properties, urbanization as well as landscape patterns might be the potential factors that influenced the earthworm community patterns.

Ecological risk assessment of polymetallic sites using weight of evidence approach.

Ecological risk assessment (ERA) of polymetallic contamination in soils has caused extensive solicitude. However, objective and feasible methods suitable for soil ERA are limited. Therefore, in this study, a multidisciplinary and quantitative weight of evidence approach (WOE) specific to soil ecosystems was developed based on the previous WOE for aquatic ecosystems. The framework consisted of four lines of evidence (LOEs): DTPA-extractable heavy metal in soils, bioaccumulation in earthworms, integration of biomarker responses and expected community effect (multi-substance Potentially Affected Fraction, msPAF). These four LOEs were initially evaluated by each hazard quotient (HQ) of them based on the ratio to the reference (RTR) of each parameter. Then, Environmental risk index (EnvRI) integrated by HQs with different weights was calculated. At last, three sites, one for reference (N1) and two for contaminated soils (N2 and N3) were chosen to apply the modified WOE approach. Results showed that heavily contaminated site, N3 had higher HQ classification for each LOE and its EnvRI was classified as Major levels, while the EnvRI of N2 was assigned into Moderate. What's more, HQ of biomarker response (HQbiomarker) integrated by RTRs of biomarkers increased gradiently with the increase of heavy metal levels in soils though irregular changes were observed for most of those biomarkers. Overall, our results indicated that the quantitative WOE framework specific to soil ERA had the advantage of obtaining a comprehensive and objective risk assessment.

Ecotoxicological effects of binary mixtures of siduron and Cd on mRNA expression in the earthworm Eisenia fetida.

This study aimed to investigate the eco-toxicological responses of earthworm (Eisenia fetida) exposed to combined siduron (herbicide) and cadmium (Cd). Eisenia fetida gene expressions including metallothionein (MT) and heat shock protein70 (Hsp70) were analyzed using real-time Polymerase Chain Reaction after individual and combined siduron (0.90, 1.80, 3.60 and 7.20µgcm-2) and Cd (0.225, 0.45, 0.90 and 1.80µgcm-2) sublethal exposures. Where, the nature of the toxicological interactions between siduron and Cd in inducing or suppressing MT and Hsp70 expression was determined by applying the Combination index (CI)-isobologram model. The results revealed significant variations in MT and weak changes in Hsp70 expression when the earthworms were exposed to individual Cd. The individual siduron exposure exhibited a significant down-regulation (p<0.01) in MT during all treatments and in Hsp70 expression only at 7.20µgcm-2 concentration; while the mixtures of siduron and Cd exposures resulted a significant down regulation (p<0.05) in both MT and Hsp70 expressions. Moreover, the combined siduron and Cd exposure revealed nearly additive effect (CI=1) at the lower effect levels and significant synergistic effect (CI<1) at the higher effect levels for both MT and Hsp70 expression. The synergistic effects of combined siduron and Cd suggest that there might be a potential risk connected to the co-occurrence of these chemicals in the environment.

The eco-toxic effects of pesticide and heavy metal mixtures towards earthworms in soil.

Earthworms are the key soil organisms, contribute to many positive ecological services that could be degraded by pesticides and other soil pollutants such as heavy metals. Chemicals usually occur as mixtures in the environmental systems which can lead synergistic effects. The assessment and characterization of soil pollutants that effects risks are very difficult due to the complexity of soil matrix, poor understanding about the fate and effects of chemical combinations like pesticide and metal mixtures in terrestrial systems, and scarcity of toxicological data on mixtures of pollutants. In this review we summarized the current studies on individual and joint effects of pesticides and metals on earthworms and indicate the mixture that cause the synergistic interactions. The review explores the methods and models used previously to evaluate the toxicity of chemical mixtures, and suggests the perspective approaches for a better knowledge of combine effects as well as research methods The summarized report indicates that pesticide and metal mixtures at all organization levels affect the earthworms negatively. Whereas, the combined pollution generated by mixtures of pesticides and metal ions could induce the DNA damage, disruption in enzyme activities, reduction in individual survival, production and growth rate, change in individual behavior such as feeding rate, and decrease in the total earthworm community biomass and density. Among the pesticides organophosphates were identified the most toxic pesticides causing the synergistic effects. The findings indicate the scarcity of toxicological data concerning the assessment of pesticide and metal mixtures at genome level; while the mechanisms causing synergism were still not sufficiently explored.

Evaluation of combined noxious effects of siduron and cadmium on the earthworm Eisenia fetida.

Environmental contaminants do not often occur as individual chemicals but rather in complex mixtures whose joint effects can create a strong toxicity to surrounding organisms. To determine the combined harmful effects of siduron (herbicide) and cadmium (heavy metal) toward Eisenia fetida earthworms, samples of worm's coelomocytes were subjected to siduron and cadmium (Cd) at sublethal concentrations (lower than LC50)-siduron 0, 0.8, 2.4, and 7.2 µg cm-2 Cd 0, 0.4, 0.8, and 1.6 µg cm-2 in filter paper contact assay, both as individual compounds and combinations. The CI-isobologram model was utilized to reveal the types of toxicological interactions between siduron and cadmium in inducing DNA damage toward earthworms. The results indicated that tail DNA percentage (TDNA %) at individual siduron and cadmium concentrations (with the exception of the lowest concentration of Cd 0.04 µgcm -2) were highly significant compared to those of the control (p < 0.01). Tail moments (TM) at individual Cd concentrations (0.8 and 1.6 µg cm -2) were highly significant compared to those of the control (p < 0.05), while the increase of TM for individual siduron was only significant (p < 0.05) at 7.2 µg cm -2 which is the highest dose/concentration of siduron used in this study. The combinations of siduron and Cd indicated a significant synergism (CI < 1) at the lower effect levels and a significant antagonism (CI > 1) at the higher effect levels. The synergistic effect for a particular combination of chemicals suggests that there might be a possible risk connected to the coincidence of these chemicals.