The effect of alexithymic characteristics on perceived stress and health anxiety during the COVID-19 pandemic.

OBJECTIVE: The anxiety of life that comes with the pandemic process increases the health anxiety and the level of perceived stress. However, there are uncertainties about which individuals are more sensitive. This study aims to investigate the effects of alexithymic characteristics on health anxiety and perceived stress. MATERIALS AND METHODS: The authors invited the participants to study via social media and e-mail. The data of 793 individuals, aged 18-65, collected over the internet (Google Forms) between November and December 2020 were statistically evaluated. Evaluations were made with the sociodemographic data form, the General Health Questionnaire-12 (GHQ-12), the Health Anxiety Scale (HAS), the Toronto Alexithymia Scale (TAS-20), and the Perceived Stress Scale (PSS). RESULTS: In mediation analyzes between TAS subscales and HAS, Difficulty in Identifying Feelings (DIF) most strongly predicted HAS (B=0.469, p<0.001) and indirectly affected HAS only through GHQ (CS: 0.08, B=0.108, SE:0.021, CI: 0.070, 0.153). However, both PSS (CS: 0.0128, B=0.084, SE:0.027, CI: 0.032, 0.139) and GHQ (CS: 0.02, B=0.139, SE:0.028, CI: 0.090, 0.198) played a mediating role between Difficulty Describing Feelings (DDF) and HAS. CONCLUSIONS: The present study suggests that individuals with alexithymic features are more sensitive to stress during periods of health-related increased stress, such as pandemics, and that individuals with alexithymic features should be given priority in psychotherapeutic interventions.

Spectrophotometric determination of cyclotrimethylenetrinitramine (RDX) in explosive mixtures and residues with the Berthelot reaction.

On-site colorimetric methods are a valuable, cost-effective tool to assess the nature and extent of contamination in remediated sites and to enable on-site screening for police criminology laboratories. The existing colorimetric method for cyclotrimethylenetrinitramine (RDX) based on a Griess reaction suffers from the non-quantitative reduction to nitrite and from the unstable character of HNO2 in acidic medium. Thus we propose a novel spectrophotometric RDX assay in explosive mixtures and residues, based on (Zn+HCl) reduction of RDX in a microwave oven, followed by neutralization of the reduction products to ammonia and low molecular-weight amines, and Berthelot reaction of these amine-compounds with phenol and hypochlorite in alkaline medium to give an intensely blue indophenol dye absorbing at 631nm. The molar absorptivity and limit of detection (LOD) for RDX were (1.08+/-0.04)x10(4) L mol(-1) cm(-1) and 0.18 mg L(-1), respectively. Application of the method to synthetic mixture solutions of RDX and trinitrotoluene (TNT) at varying proportions showed that there was minimal interference from TNT (which could be compensated for by dicyclohexylamine colorimetry), since the Berthelot reaction was essentially non-responsive to m-substituted anilines derived from TNT upon (Zn+HCl) reduction. The proposed method was successfully applied to military-purpose explosive mixtures of (RDX+inert matter) such as Comp A5, Comp C4, and Hexal P30, and to (RDX+TNT) mixtures such as Comp B. The molar absorptivity of RDX was much higher than that of either ammonium or nitrate; RDX could be effectively separated from ammonium and nitrate in soil mixtures, based on solubility differences. The Berthelot method for RDX was statistically validated using Comp B mixtures against standard HPLC equipped with a Hypersil C-18 column with (40% MeOH-60% H2O) mobile phase, and against gas chromatography-thermal energy analysis (GC-TEA) system.

Spectrophotometric determination of 4,6-dinitro-o-cresol (DNOC) in soil and lemon juice.

Although the use of once widely applied selective herbicide, 4,6-dinitro-o-cresol (DNOC), was cancelled by US-EPA in 1987, it is still found in soil and water due to its slow degradation in the environment. Since solid phase extraction-spectrophotometry combinations are much simpler and cheaper than chromatography/MS based methods and most routine laboratories lack such sophisticated instrumentation, it is desirable to establish novel sensitive, well-established, and field-applicable spectrophotometric methods for the rapid assay of DNOC in water and soil. For this purpose, two distinct spectrophotometric methods utilizing the periodate and copper(II)-neocuproine (Nc) reagents have been developed following Zn/HCl reduction of the pesticide in a microwave oven for 15s, and validated for DNOC determination at mg L(-1) level. The LOD values were 1.6 and 0.2 mg L(-1) for periodate and Cu(II)-Nc methods, respectively. Statistical comparison of the developed methods was made with the aid of high performance liquid chromatography (HPLC) equipped with a C18 (5 microm), 250 mm x 4.6 mm ID reversed phase column in conjunction with a UV (264 nm) detector, and a methanol (HPLC grade) +0.1% glacial acetic acid mixture mobile phase. Both spectrophotometric methods were directly applicable to soil since they were not interfered with common soil cations and anions, together with some pesticides. These methods were applied to real samples such as synthetically contaminated montmorillonite and lemon juice, and overall recovery efficiencies at the order of 95% or greater were achieved in the devised adsorption/elution procedures. An 8-hydroxyquinoline (oxine)-impregnated XAD copolymer resin stabilized with Fe(III) salt was used to preconcentrate DNOC at a concentration factor of 20 from lemon juice contaminated with 1 mg L(-1) DNOC, and the analyte retained at pH 2.5 was eluted with 0.025 M methanolic NaOH. Both the devised spectrophotometric methods and the proposed preconcentration column with optimized sorption and desorption conditions are novel for DNOC assay in the natural environment.