ABSTRACT
Purpose: Defining the regenerative response following various types of corneal chemical and mechanical injuries is important for understanding the pathophysiology of the injury and evaluating the effectiveness of the therapies. This study characterizes corneal epithelial healing in a murine chemical and mechanical injury model. Methods: Four groups of 10 mice each received complete corneolimbal injuries by AlgerBrush, AlgerBrush/thermal, NaOH (0.5 N), or ethanol. Slit-lamp and optical coherence tomography examinations were performed daily for 14 days. Corneal opacity (CO) and neovascularization (NV) were evaluated. The origin of the regenerated epithelium was illustrated by anti-cytokeratin 12 (K12) and anti-K13. The height of regenerated corneal epithelium and intraepithelial free nerve endings (FNEs) stained with anti-ßIII-tubulin were measured. The amount of fibrosis was measured by anti-α-smooth muscle actin (α-SMA) monoclonal antibody in the different groups. Statistical analysis was performed by ANOVA and t-test. Results: Corneal opacity and neovascularization were markedly higher in the NaOH and AlgerBrush/thermal groups. Molecular studies revealed the following: Regenerated corneal epithelium thickness was less than normal in all groups, the AlgerBrush group had the shortest height of the regenerated epithelium, ßIII-tubulin was expressed in the entire height of corneal epithelium in all groups except in the AlgerBrush group, and K12 was replaced by K13 in all groups. Conclusions: Corneal wound healing is more effective following chemical injuries in terms of epithelial thickness. Inflammation may play an important role in the outcome. Translational Relevance: Inflammation following different injuries may be redirected to be more effective in corneal regeneration and clarity.
Subject(s)
Corneal Opacity , Epithelium, Corneal , Animals , Mice , Inflammation , Sodium Hydroxide , TubulinABSTRACT
The choroid is one of the most vascularized regions of the human body. Therefore, various local and systemic physiologic/pathologic conditions and environmental factors have effects on choroidal thickness.
Subject(s)
COVID-19 , Tomography, Optical Coherence , Humans , Child , Choroid/pathology , Microvessels , Fluorescein AngiographyABSTRACT
Purpose: The Algerbrush II has been widely used to induce corneal and limbal injuries in animal models. The extent of injury varies with the duration of exposure, pressure from the placement of the burr, and the size of the burr. However, no study has explored the correlation between the duration of exposure and the severity of injury in mouse model with corneal and limbal stem cell deficiency (LSCD) induced using the Algerbrush II. Therefore, this study aimed to evaluate the variations in the severity of corneal and limbal injury with different durations of the Algerbrush II application. Methods: The entire cornea and limbus of C57BL/6 mice were injured for 30-45 s, 60-75 s, 90-120 s, and 3-4 min. Photography and slit-lamp examination was performed on days 0, 2, 4, and 7, followed by hematoxylin & eosin, periodic acid-Schiff, and immunohistochemical staining. Statistical analysis was performed using one way ANOVA analysis. Results: A duration of 30-45 s of injury was found to be sufficient to induce superficial corneal and limbal epithelial debridement and re-epithelialization was completed in all eyes by day 7; however, clinical signs of LSCD were not observed in all mice. Increasing the exposure time to 90-120 s resulted in central 2+ corneal opacity with limbal and paracentral corneal neovascularization. All eyes injured for 3-4 min displayed clinical signs of LSCD, such as persistent epithelial defects on day 7 after the injury, central corneal neovascularization, and 2.2+ diffuse corneal opacity. Histological signs of LSCD, including goblet cell metaplasia and K13 expression on the corneal surface, were observed in all injured eyes. Conclusions: Our findings suggest that the duration of injury is an important factor influencing the severity of LSCD in a murine model of injury. A 1-mm rotating burr was found to be more effective for keratectomy and pigment release, whereas a 0.5-mm burr was more suitable for corneal epithelial debridement.
Subject(s)
Corneal Diseases , Corneal Neovascularization , Corneal Opacity , Epithelium, Corneal , Limbus Corneae , Animals , Mice , Epithelium, Corneal/pathology , Corneal Neovascularization/pathology , Disease Models, Animal , Mice, Inbred C57BL , Corneal Opacity/pathology , Corneal Diseases/metabolismABSTRACT
The choroid is one of the most vascularized regions of the human body. Therefore, various local and systemic physiologic/pathologic conditions and environmental factors have effects on choroidal thickness.
Subject(s)
Migraine Disorders , Tomography, Optical Coherence , Choroid/pathology , HumansABSTRACT
This paper is a comment on the article entitled "Effect of reduced meal frequency during Ramadan fasting on retinal and choroidal thickness'' by Ersan et al. We think this paper will make a significant contribution to this article and to the literature.
