[The carbohydrate metabolism and stress hormone response during acute myocardial infarct in persons without diagnosed diabetes]. / Odezva sacharidového metabolismu a hormonu stresové odpovedi behem cerstvého srdecního infarktu u osob bez dosud prokázaného diabetu.

The authors examined 68 patients with a acute myocardial infarction without previously detected diabetes. They evaluated some indicators of the humoral stress response and the response of the carbohydrate metabolism during a recent infarction. They found very complicated interrelations between hormones of the stress reaction and indicators of the carbohydrate metabolism under conditions of circulatory stress. Despite this they detected some dominating trend and compared their findings with data from the literature.

[The value of the carbohydrate metabolism response for assessment of early prognosis in patients with acute myocardial infarct without previously detected diabetes]. / Význam reakce sacharidového metabolismu a humorální stresové odpovedi u osob s cerstvým srdecním infarktem bez dríve zjistené cukrovky, pro posouzení casné prognózy.

The authors examined 68 patients with a acute myocardial infarction without previously detected diabetes. They evaluated the importance of the relationship of the intensity of the stressor, humoral stress response and the response of the carbohydrate metabolism for the early prognosis of the infarction. Despite the complexity of these relations and heterogenity in the group of these patients, the authors confirmed the prognostic importance of hyperglycaemia, cortisolaemia and urinary catecholamine levels, and based on their own experience, they recommended to follow up these indicators during the first days after development of the infarction.

[The carbohydrate metabolism and stress hormone response in acute myocardial infarct--its importance in predicting permanent disorders of glucoregulation]. / Odezva sacharidového metabolismu a stresových hormonu behem cerstvého srdecního infarktu--význam pro predpoved trvalé poruchy glykoregulace.

The authors examined in a group of 68 patients with a acute myocardial infarction (without confirmed diabetes) some indicators of the humoral stress reaction and the response of indicators of the carbohydrate metabolism during circulatory stress. After three years they re-examined 48 of these patients with special attention to their carbohydrate metabolism. Based on the assembled results, the authors sought a relationship between findings during the acute circulatory stress and the level of the carbohydrate metabolism after three years. In future diabetics they found during the period of the acute infarction higher levels of immunoreactive insulin, a reduced IRI/blood sugar ratio and a higher excretion of urinary catecholamines. In the OGTT performed one week after the infarction they found a higher sum of C-peptide, a shift of the peak of IRI secretion and reduced IRI/blood sugar ratio. These finding could indicate the development of a permanently impaired glycoregulation and lang-term prognosis of these patients.

[Diagnosis of the heterozygote carrier state in 21-hydroxylase deficiency using steroids]. / Steroidní diagnostika heterozygotního prenasecství nedostatecnosti 21-hydroxylázy.

In an attempt to elaborate a method for screening heterozygous carriers of inborn adrenal hyperplasia caused by insufficiency of steroid 21-hydroxylase, the authors examined in 24 obligatory heterozygotes and in seven controls the concentrations of four steroids. Cortisol (F), 11 beta-hydroxyandrostendione (11-OH), 17 alpha-hydroxyprogesterone (17-OH) and androstendione (A) were estimated by the RIA method using non-commercial antisera. In the examined subjects an abbreviated ACTH test was made and the mentioned parameters were assessed at times 0, 30 and 60 minutes. The results (nmol/l) were expressed by the relationship R = F.11-OH/17-OH.A for all investigated time intervals. The coefficient R60 or difference of coefficients delta R = R60-R0 differed significantly and overlapped to a minimum extent in the group of heterozygotes and controls. The authors assume that the suggested method for the detection of carriership of 21-hydroxylase insufficiency could be used in genetic counselling.

[Changes in magnesium and ionized calcium serum levels in disorders of glucose tolerance]. / Zmeny sérové hladiny horcíku a ionizovaného vápníku pri poruchách glukózové tolerance.

In a group of 58 subjects with a strictly defined glucose tolerance (38 normal--nGT, 10 impaired glucose tolerance--pGT, 10 type II diabetics treated by diet) the authors investigated the mutual relationship between changes in the blood sugar level and insulin serum level in relation changes of calcium, ionized calcium, magnesium and total proteins. In addition to expected differences in the native blood sugar level and insulinaemia, the authors found in pGT group in reduction of ionized calcium and magnesium on fasting. In the course of the oGTT in pGT group there is a rise of ionized calcium and magnesium and thus the changes, as compared with the initial value, differ significantly from changes in subjects with a normal glucose tolerance and in type II diabetics. The differences in the pattern total calcium are less marked, the changes of total protein did not differ. Evaluation of correlations revealed a only significant relationship between the change of ionized calcium and blood sugar level after 120 mins, in DM II. The findings suggest a different calcium and magnesium metabolism after a glucose load in subjects with an impaired glucose tolerance. It is not clear whether a cause of the disorder is involved or a possible compensatory mechanism. The clinical impact will have to be examined.

The effect of verapamil and the calcium ionophore A 23187 on the calcium and phosphorus content of bone.

Verapamil was administered 30 days to adult male rats in a dose of 2 mg/rat per day and the calcium ionophore A 23187 to another group in a dose of 10 micrograms/rat per day. After verapamil, the bone calcium and phosphorus concentration rose significantly compared with the control group, whereas after ionophore A 23187 the bone calcium concentration fell statistically significantly.

Behaviour of Propionibacterium shermanii in alkaline medium.

Physiological and morphological properties of Propionibacterium shermanii were estimated in a liquid semisynthetic medium of initial pH between 7-10. Prolongation of the lag phase and a shift of the stationary phase occurred at higher initial pH values of the medium. The growth response of the strain depended on the way of glucose addition (either sterilized in the medium or added aseptically after sterilization). At pH 8.5 and higher the cells begin to form slime and capsules. The strain exhibited growth activity even at initial pH values of the medium 11.5