ABSTRACT
No disponible
Subject(s)
Humans , Female , Child , Coronary Vessels , Coronary Vessel Anomalies/complications , Coronary Vessel Anomalies/drug therapy , Coronary Vessel Anomalies/genetics , Pulmonary Artery , Midazolam/therapeutic use , Myocardial Contraction , Electrocardiography , Radiography, Thoracic/trends , Radiography, Thoracic , Furosemide/therapeutic use , Cardiotonic Agents/therapeutic useSubject(s)
Head and Neck Neoplasms/surgery , Infant, Premature, Diseases/surgery , Teratoma/surgery , Airway Obstruction/congenital , Airway Obstruction/etiology , Airway Obstruction/surgery , Airway Obstruction/therapy , Cardiopulmonary Resuscitation , Cesarean Section , Diagnosis, Differential , Female , Fetal Diseases/diagnosis , Head and Neck Neoplasms/congenital , Head and Neck Neoplasms/diagnosis , Head and Neck Neoplasms/embryology , Hemangioma/diagnosis , Humans , Infant, Newborn , Infant, Premature , Infant, Premature, Diseases/diagnosis , Infant, Premature, Diseases/embryology , Intubation, Intratracheal , Lymphangioma/diagnosis , Prenatal Diagnosis , Teratoma/congenital , Teratoma/diagnosis , Teratoma/embryologyABSTRACT
No disponible
No disponible
Subject(s)
Female , Infant, Newborn , Humans , Teratoma/surgery , Head and Neck Neoplasms/surgery , Airway Obstruction/etiology , Diagnosis, Differential , Hemangioma/diagnosis , Intubation, Intratracheal , Lymphangioma/diagnosis , Prenatal DiagnosisABSTRACT
The cytokine rumor necrosis factor-alpha (TNF-alpha) plays a prominent role in the inflammatory response and has been quantified in several kinds of body fluids. A safe method for keeping samples under the best storage conditions for later studies is clearly needed. We estimated TNF-alpha shelf life by using an accelerated stability testing protocol based on the Arrhenius equation. We investigated two kinds of samples: cerebrospinal fluid (CSF) normal pool (from patients without meningitis) and CSF pathological pool (from patients with pyogenic meningitis). Results of the stress protocol indicated that storing both normal and pathological CSF samples at -70 degrees C would maintain sample stability-i.e., retain at least 90% of the original TNF-alpha content-for approximately 5 years. At -20 degrees C and 4 degrees C, the projected stability time at which the same recovery would be obtained was 190 and 90 days, respectively.
Subject(s)
Tumor Necrosis Factor-alpha/cerebrospinal fluid , Drug Stability , Humans , Meningitis/cerebrospinal fluid , Thermodynamics , Time FactorsABSTRACT
We evaluated the technical performance of two new commercial automated immunoassay systems of third-generation assays for thyrotropin. The interassay CV was 2.8% for AutoDELFIA and 3.25% for Access at thyrotropin concentrations of approximately 1.3 mIU/l. The lower detection limits of the assays were 0.023 mIU/l for AutoDELFIA and 0.0096 mIU/l for Access, and the functional sensitivity for a CV of 20% was 0.027 mIU/l and 0.028 mIU/l, respectively. Sample to sample carry over was negligible (0.0016% for AutoDELFIA and 0.005% for Access). The range of linearity was acceptable for Access (102-115%) but not for low thyrotropin concentrations in AutoDELFIA (143% for a thyrotropin value of 0.48 mIU/l). Correlation between AutoDELFIA and Access was adequate (r = 0.999). We conclude that both automated immunoassays offer good reliability, practicability and performance characteristics.
