ABSTRACT
This work aimed to determine the presence of bacterial pathogens in fish with a clinical picture suggestive of infectious disease in Nile tilapia reared in Chiapas, Mexico. Blood and viscera samples were taken from healthy and diseased animals from commercial farms. Clinical and pathological examinations of each individual were performed and samples were collected for bacteriological studies. The bacterial isolates were identified and characterized by culture, biochemical tests, antibiogram, challenge tests and 16S rRNA sequencing. Staphylococcus haemolyticus and Providencia vermicola were isolated from various diseased organisms. The clinical picture caused by Staphylococcus haemolyticus was characterized by appetite disorders, neurological signs, nodulation or ulceration in different areas and congestion or enlargement of internal organs. Providenciosis in juvenile specimens caused a characteristic picture of hemorrhagic septicemia. Challenge tests performed in healthy organisms revealed that both infections caused higher mortality rates in fish (p < 0.05) compared with non-infected specimens, with 100% survival. There was 100% mortality for animals infected with P. vermicola after three days post infection and 45% for those infected with S. haemolyticus. The isolation and identification of two pathogens involved in an infection process were achieved and cataloged as potential causal agents of disease outbreaks in tilapia farming in Mexico. This is the first report of possible bacterial infection caused by S. haemolyticus and P. vermicola in tilapia farms, which are two uncommon but potentially emerging pathogens for the species.
ABSTRACT
Objective: Secondary urinary bladder tumors account for 2% of neoplasms in this location. Melanoma is the neoplasm that mostfrequently produces distant metastases in the bladder. Despite its low prevalence, it should be a possible diagnosis to considerfacing urological symptoms, due to the existence of targeted therapies. Method: We present two cases of uveal melanoma metastasis in the bladder, their characteristics, and a review of the literature. Result: In this paper we present the histological characteristics and complementary techniques for the diagnosis of bladder metastases of melanoma and propose their inclusion among the possible differential diagnoses for bladder neoplasms. Conclusions: Given that there are currently targeted therapies against melanoma, the relevance of their inclusion in the differential diagnosis of bladder tumors stands out. (AU)
Objetivo: La vejiga urinaria no es un órgano frecuente de metastasis a distancia. Sin embargo, se puedever afectada secundariamente en un 2% de todas las neoplasias. Dicha afectación se produce habitualmente por extensión local de otros tumores, aunque, ciertas neoplasias pueden producir metástasis a distancia en la vejiga, como es el caso del melanoma. A pesar de esta baja prevalencia, los antecedentes personales del paciente deben ser tenido sen cuenta en el diagnóstico de la neoplasia vesical. Método: Se presentan dos casos de metástasis de melanoma uveal en vejiga, sus características y una revisiónde la literatura.Resultado: En este trabajo se exponen las características histológicas y las técnicas complementarias para el diagnóstico de las metástasis de melanoma y se resalta su importancia en el diagnóstico de las neoplasias vesicales. Conclusiones: Dado que actualmente existen terapiasdirigidas frente al melanoma, se destaca la relevancia de suinclusión en el diagnóstico diferencial de los tumores de lavejiga. (AU)
Subject(s)
Humans , Male , Aged , Uveal Neoplasms/pathology , Urinary Bladder Neoplasms/secondary , Melanoma/secondary , Urinary Bladder Neoplasms/diagnosis , Melanoma/diagnosis , Diagnosis, DifferentialABSTRACT
OBJECTIVE: Secondary urinary bladder tumors account for 2% of neoplasms in this location. Melanoma is the neoplasm that most frequently produces distant metastases in the bladder. Despite its low prevalence, it should be a possible diagnosis to consider facing urological symptoms, due to the existence of targeted therapies. METHOD: We present two cases of uveal melanoma metastasis in the bladder, their characteristics, and a review of the literature. RESULT: In this paper we present the histological characteristics and complementary techniques for the diagnosis of bladder metastases of melanoma and propose their inclusion among the possible differential diagnoses for bladder neoplasms. CONCLUSIONS: Given that there are currently targeted therapies against melanoma, the relevance of their inclusion in the differential diagnosis of bladder tumors stands out.
Subject(s)
Melanoma , Uveal Neoplasms , Humans , Urinary Bladder , Melanoma/pathology , Melanoma/secondary , Melanoma/therapy , Uveal Neoplasms/pathology , Uveal Neoplasms/therapy , Neoplasm MetastasisABSTRACT
OBJECTIVE: Two cases of metanephric adenoma are presented, a rare benign renal tumor, and a literature review is done under the current WHO classification (2016). METHODS: Standard histopathological study was performed with hematoxylin-eosin and immunohistochemistry to analyze the expression of WT, Vimentin, Racemase, CK7, CD10 and RCC. RESULTS: Neoplasms of 3 and 4.5 cm, histologically, exhibiting tubulopapillary architecture. There was no evidence of significant nuclear atypia and mitotic figures. Immunohistochemical study showed positive immunoreaction for WT1 and Vimentin in tumor cells. CONCLUSIONS: Two new cases of metanephric adenoma are presented and a review of the literature was performed in order to discuss the prognosis and differential diagnosis of metanephric adenoma. This is a rare tumor and its diagnosis lies on its morphology and its immunohistochemical profile.
Subject(s)
Adenoma , Kidney Neoplasms , Adenoma/pathology , Adenoma/surgery , Adolescent , Female , Humans , Kidney Neoplasms/pathology , Kidney Neoplasms/surgery , Male , Middle AgedABSTRACT
OBJETIVO: Presentamos 2 casos de adenoma metanéfrico, un tumor renal benigno poco frecuente y realizamos una revisión de la literatura bajo la clasificación actual de la OMS (2016). MÉTODOS: Se realizaron cortes de bloques de parafina para tinción con HE y técnicas inmunohistoquímicas para analizar la expresión de WT, Vimentina, Racemasa, CK7, CD10 y RCC. RESULTADOS: Las neoplasias de 3 y 4,5 cm, histológicamente mostraban arquitectura tubular y papilar. No se evidenció atipia nuclear significativa ni figuras de mitosis. El estudio inmunohistoquímico demostró inmunorreacción positiva de las células tumorales para WT1 y Vimentina. CONCLUSIONES: Presentamos 2 nuevos casos de adenoma metanéfrico y realizamos revisión de la literatura que hay al respecto para discutir la evolución y el diagnóstico diferencial del adenoma metanéfrico. Este es un tumor poco frecuente y su diagnóstico reside en su morfología y perfil inmunohistoquímico
OBJECTIVE: Two cases of metanephric adenoma are presented, a rare benign renal tumor, and a literature review is done under the current WHO classification (2016). METHODS: Standard histopathological study was performed with hematoxylin-eosin and immunohistochemistry to analyze the expression of WT, Vimentin, Racemase, CK7, CD10 and RCC. RESULTS: Neoplasms of 3 and 4.5 cm, histologically, exhibiting tubulopapillary architecture. There was no evidence of significant nuclear atypia and mitotic figures. Immunohistochemical study showed positive immunoreaction for WT1 and Vimentin in tumor cells.CONCLUSIONS: Two new cases of metanephric adenoma are presented and a review of the literature was performed in order to discuss the prognosis and differential diagnosis of metanephric adenoma. This is a rare tumor and its diagnosis lies on its morphology and its immunohistochemical profile
Subject(s)
Humans , Male , Female , Adolescent , Middle Aged , Kidney Neoplasms/pathology , Adenoma/pathology , Immunohistochemistry/methods , Diagnosis, Differential , Hematuria/etiology , Polycythemia/etiologySubject(s)
Adenocarcinoma/secondary , Bone Neoplasms/secondary , Prostatic Neoplasms/pathology , Aged , Humans , MaleABSTRACT
Introduction: Synovial sarcoma is an aggressive neoplasm described in several locations but is uncommon in the lung. Method: We report a case of biphasic synovial sarcoma of the lung stressing the histologic and immunohistochemical characteristics, genetics and differential diagnosis. Results: a biphasic pattern and SYT-SSX translocation were demostrated. Comments: histology and immuno-histochemistry lead to diagnosis most of the times but the typical translocation is definitive.
Introducción: El sarcoma sinovial es una neoplasia agresiva que aun habiendo sido descrita en múltiples localizaciones, resulta sumamente infrecuente en localización pulmonar. Método: Presentamos un caso de sarcoma sinovial bifásico de localización pulmonar haciendo hincapié en sus características histológicas inmunohistoquímicas y genéticas así como en su diagnóstico diferencial. Resultados: La neoplasia mostraba un patrón bifásico bien caracterizado. El estudio genético demostró la translocación SYT-SSX. Comentarios: Si bien la histología y la inmunohistoquímica permiten en la mayoría de los casos el diagnóstico del sarcoma sinovial, es la translocación genética la que define verdaderamente esta entidad.
Subject(s)
Humans , Male , Middle Aged , Lung Neoplasms/diagnosis , Lung Neoplasms/genetics , Lung Neoplasms/pathology , Sarcoma, Synovial/diagnosis , Sarcoma, Synovial/genetics , Sarcoma, Synovial/pathology , Diagnosis, Differential , Immunohistochemistry , Oncogene Proteins, Fusion , Polymerase Chain Reaction , Translocation, GeneticABSTRACT
OBJECTIVES: Ureteral endometriosis is a rare entity which may produce urinary tract obstruction with subsequent loss of renal function if treatment is not undertaken early due to its asymptomatic but locally aggressive course. METHODS/RESULTS: We report one case of intrinsic ureteral endometriosis with unilateral renal failure (renal atrophy) and unresectable periureteral fibroinflammatory thickening involving vascular or visceral structures. CONCLUSIONS: We emphasize the diagnostic and therapeutic importance of a high degree of clinical suspicion about this entity, mainly in patients with history of genital endometriosis.
Subject(s)
Endometriosis/diagnosis , Ureteral Diseases/diagnosis , Female , Humans , Middle AgedABSTRACT
OBJETIVOS: La endometriosis ureteral es una entidad poco frecuente que debido a su curso asintomáticopero a la vez localmente agresivo puede originar un cuadro de obstrucción urinaria con la subsiguiente pérdida de la función renal si no se instaura un tratamiento precozmente.MÉTODO/RESULTADOS: Presentamos un caso de endometriosisureteral intrínseca acompañado de insuficiencia renal unilateral (atrofia renal) y plastrón fibroinflamatorio periureteral irresecable al englobar estructuras vasculares y viscerales.CONCLUSIONES: Destacamos la importancia diagnósticay terapéutica que conlleva un alto índice de sospecha clínica de esta entidad sobre todo en aquellas pacientes con antecedentes de endometriosis genital
OBJECTIVES: Ureteral endometriosis is a rare entity which may produce urinary tract obstruction with subsequent loss of renal function if treatment is not undertaken ;;early due to its asymptomatic but locally aggressive course. ;;METHODS/RESULTS: We report one case of intrinsic ureteral endometriosis with unilateral renal failure (renal atrophy) and unresectable periureteral fibroinflammatory thickening involving vascular or visceral structures. ;;CONCLUSIONS: We emphasize the diagnostic and ;;therapeutic importance of a high degree of clinical suspicion about this entity, mainly in patients with history of genital endometriosis
Subject(s)
Female , Middle Aged , Humans , Endometriosis/diagnosis , Urethral Diseases/diagnosisABSTRACT
Myofibrosarcoma is a controversial neoplasm composed of cells with differentiation toward myofibroblasts. The authors report an unusual case of myofibrosarcoma in which, in addition to the characteristic features reported, tumor cells contained intracytoplasmic hyaline (fibroma-like) inclusion bodies. A 66-year-old man complained of a painless enlarged mass in his right shoulder over the previous 6 months. The tumor recurred 2 years after operation. On histologic examination, the tumor displayed diffusely infiltrative growth with isolation of individual skeletal muscle fibers. The tumor cells were most often arranged in an intersecting (herringbone) fascicular pattern but a vaguely storiform pattern was also observed. Cellularity varied from one area to another. The cells were spindle-shaped, with ill-define pale eosinophilic cytoplasm, and a wavy or tapering nucleus with fine chromatin and small nucleoli. Intracytoplasmic hyaline inclusion bodies of variable size, often located adjacent to the nucleus, were visible in numerous spindle and stellate cells. The mitotic index was 3/10 HPF. The tumor was scored as grade 1. Immunohistochemistry revealed positive staining to muscle-specific actin, desmin and vimentin in most tumor cells. Intracytoplasmic hyaline inclusion bodies did not react specifically to any one antibody, but showed a marked ring-like immune reaction, particularly to muscle-specific actin. Electron microscopy showed tumor cells with indented nuclei and small nucleoli, abundant rough endoplasmic reticulum, micropinocytotic vesicles, and longitudinally arranged fine filaments with focal electron-dense patches and subplasmalemmal plaques. The most striking feature was the presence of large, globular or ball-like, non-membrane-bound, randomly scattered clusters of fine filament, usually adjacent to the nucleus.
