ABSTRACT
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.
Subject(s)
Dioxygenases , Genes, Bacterial , Hydrocarbons, Aromatic/metabolism , Pseudomonas/genetics , Soil Microbiology , Argentina , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Biodegradation, Environmental , Catechol 1,2-Dioxygenase , Catechol 2,3-Dioxygenase , DNA, Bacterial/genetics , Enzyme Induction , Naphthalenes/metabolism , Oxygenases/genetics , Oxygenases/metabolism , Polymerase Chain Reaction , Protocatechuate-3,4-Dioxygenase/genetics , Protocatechuate-3,4-Dioxygenase/metabolism , Pseudomonas/classification , Pseudomonas/enzymology , Pseudomonas/isolation & purification , Soil Pollutants/metabolismABSTRACT
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.
Subject(s)
Genes, Bacterial , Hydrocarbons, Aromatic/metabolism , Pseudomonas , Soil Microbiology , Argentina , Biodegradation, Environmental , DNA, Bacterial , Enzyme Induction , Naphthalenes , Oxygenases , Polymerase Chain Reaction , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Protocatechuate-3,4-Dioxygenase , Pseudomonas , Soil PollutantsABSTRACT
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.(AU)
Subject(s)
Comparative Study , Genes, Bacterial , Hydrocarbons, Aromatic/metabolism , Pseudomonas/genetics , Soil Microbiology , Argentina , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , DNA, Bacterial/genetics , Enzyme Induction , Naphthalenes/metabolism , Oxygenases/genetics , Oxygenases/metabolism , Polymerase Chain Reaction , Protocatechuate-3,4-Dioxygenase/genetics , Protocatechuate-3,4-Dioxygenase/metabolism , Pseudomonas/classification , Pseudomonas/enzymology , Pseudomonas/isolation & purification , Soil Pollutants/metabolismABSTRACT
Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.
