Plant-minerals-water interactions: An investigation on Juncus acutus exposed to different Zn sources.

Juncus acutus has been proposed as a suitable species for the design of phytoremediation plans. This research aimed to investigate the role played by rhizosphere minerals and water composition on Zn transformations and dynamics in the rhizosphere-plant system of J. acutus exposed to different Zn sources. Rhizobox experiments were conducted using three different growing substrates (Zn from 137 to 20,400 mg/kg), and two irrigation lines (Zn 0.05 and 180 mg/l). The plant growth was affected by the substrate type, whereas the Zn content in the water did not significantly influence the plant height for a specific substrate. J. acutus accumulated Zn mainly in roots (up to 10,000 mg/kg dw); the metal supply by the water led to variable increases in the total Zn concentration in the vegetal organs, and different Zn distributions both controlled by the rhizosphere mineral composition. Different Zn complexation mechanisms were observed, mainly driven by cysteine and citrate compounds, whose amount increased linearly with Zn content in water, but differently for each of the investigated systems. Our study contributes to gain a more complete picture of the Zn pathway in the rhizosphere-plant system of J. acutus. We demonstrated that this vegetal species is not only capable of developing site-specific tolerance mechanisms, but it is also capable to differently modulate Zn transformation when Zn is additionally supplied by watering. These findings are necessary for predicting the fate of Zn during phytoremediation of sites characterized by specific mineralogical properties and subject to water chemical variations.

Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters.

In order to increase the knowledge about geo-bio interactions in extreme metal-polluted mine waters, we combined microbiological, mineralogical, and geochemical analyses to study the indigenous sulfate-reducing bacteria (SRB) involved in the heavy metal (HM) biomineralization processes occurring in Iglesiente and Arburese districts (SW Sardinia, Italy). Anaerobic cultures from sediments of two different mining-affected streams of this regional framework were enriched and analyzed by 16S rRNA next-generation sequencing (NGS) technique, showing sequences closely related to SRB classified in taxa typical of environments with high concentrations of metals (Desulfovibrionaceae, Desulfosporosinus). Nevertheless, the most abundant genera found in our samples did not belong to the traditional SRB groups (i.e., Rahnella, Acinetobacter). The bio-precipitation process mediated by these selected cultures was assessed by anaerobic batch tests performed with polluted river water showing a dramatic (more than 97%) Zn decrease. Scanning electron microscopy (SEM) analysis revealed the occurrence of Zn sulfide with tubular morphology, suggesting a bacteria-mediated bio-precipitation. The inocula represent two distinct communities of microorganisms, each adapted to peculiar environmental conditions. However, both the communities were able to use pollutants in their metabolism and tolerating HMs by detoxification mechanisms. The Zn precipitation mediated by the different enriched cultures suggests that SRB inocula selected in this study have great potentialities for the development of biotechnological techniques to reduce contaminant dispersion and for metal recovery.