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1.
Genet Mol Biol ; 38(1): 48-54, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25983624

ABSTRACT

In this study, we genetically characterized the Uruguayan pig breed Pampa Rocha. Genetic variability was assessed by analyzing a panel of 25 microsatellite markers from a sample of 39 individuals. Pampa Rocha pigs showed high genetic variability with observed and expected heterozygosities of 0.583 and 0.603, respectively. The mean number of alleles was 5.72. Twenty-four markers were polymorphic, with 95.8% of them in Hardy Weinberg equilibrium. The level of endogamy was low (FIS = 0.0475). A factorial analysis of correspondence was used to assess the genetic differences between Pampa Rocha and other pig breeds; genetic distances were calculated, and a tree was designed to reflect the distance matrix. Individuals were also allocated into clusters. This analysis showed that the Pampa Rocha breed was separated from the other breeds along the first and second axes. The neighbour-joining tree generated by the genetic distances DA showed clustering of Pampa Rocha with the Meishan breed. The allocation of individuals to clusters showed a clear separation of Pampa Rocha pigs. These results provide insights into the genetic variability of Pampa Rocha pigs and indicate that this breed is a well-defined genetic entity.

2.
Anal Bioanal Chem ; 385(5): 931-6, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16791574

ABSTRACT

Visible (Vis) and near-infrared reflectance (NIR) spectroscopy combined with chemometrics was explored as a tool to trace muscles from autochthonous and crossbreed pigs from Uruguay. Muscles were sourced from two breeds, namely, the Pampa-Rocha (PR) and the Pampa-Rocha x Duroc (PRxD) crossbreed. Minced muscles were scanned in the Vis and NIR regions (400-2,500 nm) in a monochromator instrument in reflectance. Principal component analysis (PCA), discriminant partial least square regression (DPLS), linear discriminant analysis (LDA) based on PCA scores and soft independent modelling of class analogy (SIMCA) were used to identify the origin of the muscles based on Vis and NIR data. Full cross validation was used as validation method when classification models were developed. DPLS correctly classified 87% of PR and 78% of PRxD muscle samples. LDA calibration models correctly classified 87 and 67% of muscles as PR and PRxD, respectively. SIMCA correctly classified 100% of PR muscles. The results demonstrated the usefulness of Vis and NIR spectra combined with chemometrics as rapid method for authentication and identification of muscles according to the breed of pig.


Subject(s)
Muscles/chemistry , Spectroscopy, Near-Infrared/methods , Animals , Feasibility Studies , Pedigree , Sus scrofa , Uruguay
3.
Aten Primaria ; 9(1): 13-5, 17-8, 1992 Jan.
Article in Spanish | MEDLINE | ID: mdl-1308442

ABSTRACT

AIMS: To find out the personal characteristics of patients who receive intra-muscular medication. To examine those illnesses where this treatment is used. To compare the number of intra-muscular treatments prescribed with the nature of the medication, according to a model of Primary Care and to the doctors training. DESIGN: We are dealing with an observational study, in which a questionnaire was used to find patients' characteristics and the prescription given. SITE. The present study was carried out in the Ibiza Health Centre and two old-fashioned out-patient clinics in our Health Area. PATIENTS AND OTHER PARTICIPANTS: The population under study consisted of all those patients above the age of 7 who received intramuscular treatment during the period of the study (October, November and December, 1990). INTERVENTIONS: There were no interventions, as this was an observational study. MEASUREMENTS AND MAIN FINDINGS: The intra-muscular method of administration was used more in women, people over 50 and those with a very low cultural level. The majority of patients (59.66%) believed that this method was superior to enteric treatments. This method was more often used in out-patient clinics than in the Health Centre (p < 0.01). In the Health Centre less medication treating the Locomotive System (p < 0.001) and more dealing with the Centre Nervous System (p < 0.02) was prescribed. In out-patient clinics more antibiotics, either in combination with each other or with other products, were prescribed (p < 0.01). CONCLUSIONS: In the Health Centre the intra-muscular method was used less. Additionally, a smaller proportion of the medication given was intrinsically useless or of unacceptably low value.


Subject(s)
Drug Prescriptions , Pharmaceutical Preparations/administration & dosage , Adolescent , Adult , Age Factors , Aged , Chi-Square Distribution , Child , Drug Prescriptions/statistics & numerical data , Drug Utilization/statistics & numerical data , Female , Humans , Injections, Intramuscular/statistics & numerical data , Male , Middle Aged , Sex Factors , Spain/epidemiology
4.
Rev Chil Pediatr ; 61(6): 337-41, 1990.
Article in Spanish | MEDLINE | ID: mdl-2152221

ABSTRACT

As an approach to measurement of the importance of chronic diseases in childhood, type and frequency of diagnoses in children admitted to the pediatric wards of a general metropolitan hospital at Santiago, Chile, were reviewed and recorded along hospitalization and at the time of discharge from March 1 through June 30, 1989 (n: 426). Newborns were excluded. Main problems of the study were lacks of uniform national criteria to define chronic illness and of modern technology to certify diagnoses. Ninety five hospital discharged children (23.3%) were considered to have a definitive, confirmed chronic disease (CCD), other 51 (12%) were cases of possibly chronic disease (PCD) while the remainder were thought to be carriers of acute illness. Among 146 patients taken as CCD or PCD cases, 126 (87.7%) were considered to have single organic system diseases as defined by areas of medical interest or specialty; other 3 cases (8.9%) had two affected systems and 5 children (3.4%) had three or more involved systems. More frequently affected systems (in number of cases) and their corresponding proportions of CCD were as follows: neurologic (31 cases and 58.1% CCD), oncohematologic (28 cases and 96.4% CCD) and gastrointestinal (26 cases and 26.9% CCD). More extensive studies, covering other medical care providing settings are desirable and necessary to measure the magnitude and features of chronic disease entities in chilean childhood.


Subject(s)
Chronic Disease/epidemiology , Age Factors , Child , Child, Preschool , Chile/epidemiology , Female , Gastrointestinal Diseases/epidemiology , Hematologic Diseases/epidemiology , Hospitalization , Humans , Infant , Infant, Newborn , Male , Nervous System Diseases/epidemiology
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