A new isolate cold-adapted Ankistrodesmus sp. OR119838: influence of light, temperature, and nitrogen concentration on growth characteristics and biochemical composition using the two-stage cultivation strategy.

Natural-based chemicals from microalgae such as lipids and pigments are the interests in industries and the bioeconomy. Cold-adapted Ankistrodesmus sp. OR119838, an isolated strain from Cheshmeh-Sabz Lake in northeastern Iran, was cultivated using a two-stage culture strategy under different environmental conditions. With doubling the nitrate concentration at the vegetative stage (170 mg/L) and increasing the light intensity (180 µmol photons/m2/s) the highest specific growth rate (0.61 ± 0.02 per day) and biomass productivity (121.1 ± 7.2 mg/L/day) were observed at 25 °C. In the optimal growth condition Chl a and Chl b contents of Ankistrodesmus sp. OR119838 reached the highest amount (11.07 ± 0.14 and 11.23 ± 0.29 µg/mL, respectively) at 25 °C. While carotenoid content correlated negatively with optimum biomass productivity (- 0.708) and had the best value (12.23 ± 0.29 µg/mL) in nitrogen deficiency (42 mg/L) and intense light conditions (180 µmol photons/m2/s) at 15 °C. Lipid content was increased with declined nitrate concentration (42 mg/L), high light intensity, and 180 µmol photons/m2/s at 25 °C. The highest percentage of polyunsaturated fatty acids (71.94%) and α-linolenic acid (57.73 ± 6.63%) was observed in conditions with 170 mg/L nitrate concentration and low light intensity (40 µmol photons/m2/ s) at the low temperature (15 °C). While saturated fatty acids content (43.27%) and palmitic acid reached the highest amount under 40 µmol photons/m2/s, 42 mg/L nitrate at 25 °C (35.02 ± 5.33%). Biomass productivity of Ankistrodesmus sp. OR119838, as a cold-adapted strain, decreased by only 8.2% with a 10-degree decline in temperature. Therefore, this strain has good potential to grow in open ponds by tolerating the daily temperature fluctuations.

Minimal climate change impacts on the geographic distribution of Nepeta glomerulosa, medicinal species endemic to southwestern and central Asia.

Medicinal plants are valuable species, but their geographic distributions may be limited or exposed to extinction by climate change. Therefore, research on medicinal plants in the face of climate change is fundamental for developing conservation strategies. Distributional patterns for a semi-endemic medicinal plant species, Nepeta glomerulosa, distributed in southwestern and central Asia was determined based on a maximum-entropy algorithm. We evaluated potential geographic shifts in suitability patterns for this species under two Shared Socioeconomic Pathways scenarios (SSP2-4.5 and SSP5-8.5) of climate change for 2060. Our models based on climatic features indicate that the species occupies montane areas under current conditions; transfer of the model to future climate scenarios indicated that suitable areas for the species will increase in general, and the species will likely track its favored set of climate conditions. But the types and degrees of these changes differ among areas. Our findings can be used to inform conservation management programs for medicinal, endemic, and endangered species that probably respond similarly to climate change in southwestern and central Asia.

Plant community responses to environmentally friendly piste management in northeast Iran.

It is well-known that pistes have adverse effects on alpine ecosystems. Previous studies urged that pistes should be installed and managed in the ways to minimize negative impacts on natural habitats. However, the impacts of this type of management on the plant communities are not widely studied. The aim of this study was to examine species composition and biodiversity changes in an environmentally friendly managed piste in northeast Iran. This piste has been established in a previously degraded alpine landscape. For the vegetation survey, we sampled 44 within and 28 off-piste plots. Except for the piste management, other environmental factors were similar between the piste and off-piste plots. Dominant species were determined, and variation in community composition of the two areas was visualized. Also, native species, phylogenetic, and functional Hill diversity of the two areas were compared. The results showed that there was a moderate differentiation in the species composition of the piste and off-piste. Two palatable species (i.e., Bupleurum falcatum and Melica persica) were dominant in the piste and were not recorded in the off-piste. The diversity calculations results showed that the species diversity of the piste was higher than that of the off-piste. Phylogenetic diversity at the level of frequent and dominant plants showed a similar result. The piste had a higher functional diversity in terms of functional richness, and functional diversity of frequent and dominant plants. Our findings imply, after 10 years, species, phylogenetic, and functional diversity of the piste is significantly improved. Environmentally friendly piste management (EFPM) induced species composition change that led to emerging species that were absent in the off-piste. We can conclude that EFPM led to restoration of a degraded landscape. Long-term impacts of EFPM are still unknown, therefore, caution should be undertaken regarding the installation of new environmentally friendly pistes in other areas.

Plant-plant interactions influence phylogenetic diversity at multiple spatial scales in a semi-arid mountain rangeland.

Molecular phylogenies are increasingly used to understand how biotic interactions and environment shape phylogenetic community structure (PCS). However, we do not understand the effects of plant-plant interactions and environment on PCS and phylogenetic diversity across spatial scales, particularly in rangelands. Here, we ask: (1) do plant-plant interactions and environment affect PCS and phylogenetic diversity differently across the three spatial scales of the patch, the community, and the habitat? (2) What are the impacts of dominant cushion-nurse plants on the phylogenetic structure of plant communities? We assessed the PCS of semi-arid plant communities along an elevation gradient at the patch, community and habitat scales. Then, we assessed co-occurrence patterns along two sample slopes. Our results indicated important roles for biotic interactions and environmental filtering in determining phylogenetic diversity, with biotic interactions, in particular, having a stronger tendency to increase phylogenetic diversity. This is most likely due to the asymmetrical effects of nurse plants across the three spatial scales on our two different slopes. The impact of biotic interactions caused non-random phylogenetic patterns in more severe environments. In conclusion, biotic interactions influence phylogenetic diversity by altering PCS across aspects and along elevation gradients.

Spatial scale-dependent phylogenetic signal in species distributions along geographic and elevation gradients in a mountainous rangeland.

The mechanisms determining community phylogenetic structure range from local ecological mechanisms to broad biogeographical processes. How these community assembly processes determine phylogenetic structure and patterns in rangeland communities across multiple spatial scales is still poorly understood. We sought to determine whether the structure of herbaceous and shrub assemblages along local environmental gradients (elevation) and broad geography (latitude) exhibited phylogenetic signal at different spatial scales, across 2,500 ha of a mountainous rangeland. We analyzed species distribution and phylogenetic data at two spatial scales: the community level (1 m2 sample units obtained by stratified random sampling) and the habitat level (plant assemblages identified categorically based on environmental and geographical variables). We found significant phylogenetic signal in structure and pattern at both spatial scales, along local elevational, and latitudinal gradients. Moreover, beta diversity was affected by different environmental variables in herbaceous and shrub species distributions across different spatial scales. Our results highlight the relative importance of local ecological mechanisms, including niche-based deterministic processes (environmental filtering and species interactions) as well as those of biogeographical processes, such as stochastic dispersal limitation and habitat specialization in plant assemblages of mountainous rangeland.

Phylogenetic relationships among diploid species of Symphyotrichum (Asteraceae: Astereae) based on two nuclear markers, ITS and GAPDH.

The mostly North American subtribe Symphyotrichinae (Asteraceae: Astereae) comprises Canadanthus, Ampelaster, Psilactis, Almutaster, and Symphyotrichum. Intergeneric and interspecific relationships within the subtribe have been investigated in the past, particularly by Nesom [Nesom, G.L., 1994. Review of the taxonomy of Aster sensu lato (Asteraceae: Astereae), emphasizing the new world species, Phytologia 77, 141-297] and Semple [Semple, J.C., 2005. Classification of Symphyotrichum. Available from: ], using morphological and cytological approaches. Symphyotrichum is the largest and most complex genus within the subtribe and includes four subgenera: Symphyotrichum (x=7, 8), Virgulus (x=4, 5), Astropolium (x=5), and Chapmaniani (x=7). In this study we used two nuclear markers, the nrDNA internal transcribed spacer (ITS) and the low-copy nuclear gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH), to resolve intergeneric and interspecific relationships within the subtribe at the diploid level, and to determine whether our phylogenies validate the classifications of Nesom or Semple. Our results confirm the distinct generic status of Canadanthus and Ampelaster, whereas Psilactis and Almutaster form a polytomy with Symphyotrichum. Within Symphyotrichum, subg. Virgulus is monophyletic based on ITS and appears polyphyletic based on GAPDH. Neither the ITS nor the GAPDH analyses support a distinct status for subg. Astropolium, which groups within subg. Symphyotrichum. In general, interspecific relationships within Symphyotrichum are unresolved. Lack of resolution may be interpreted as a case of recent and rapid evolutionary radiation.