ABSTRACT
Adverse pregnancy outcomes have been associated with the presence of glyphosate (G) in umbilical cord, serum, and urine samples from pregnant women. Our aim was to study the effect of G on blastocyst implantation using an in vitro mouse model, and the migration and acquisition of endothelial phenotype of the human trophoblastic HTR8/SVneo (H8) cells. In mouse blastocysts, no differences in attachment time and implantation outgrowth area were observed after G exposure. H8 cell migration was stimulated by 0.625 µM G without cytotoxicity. After 6 h, the mRNA expression of vascular endothelial growth factor (VEGF) and C-C motif chemokine ligand 2 (CCL2) was upregulated in H8 cells exposed to 1.25 µM G when compared vehicle-treated cells (p ≤ 0.05). No differences were observed in interleukin 11, VEGF receptor 1, and coagulation factor II thrombin receptor in H8 cells exposed to different concentrations of G for 6 h compared to the vehicle. Interestingly, exposure to G did not alter angiogenesis as measured by a tube formation assay. Taken all together, these results suggest that G exposure may contribute as a risk factor during pregnancy, due to its ability to alter trophoblast migration and gene expression.
Subject(s)
Blastocyst , Cell Movement , Embryo Implantation , Glycine , Glyphosate , Trophoblasts , Trophoblasts/drug effects , Trophoblasts/metabolism , Cell Movement/drug effects , Humans , Animals , Female , Mice , Glycine/analogs & derivatives , Glycine/toxicity , Glycine/pharmacology , Blastocyst/drug effects , Blastocyst/metabolism , Embryo Implantation/drug effects , Neovascularization, Physiologic/drug effects , Cell Line , Vascular Endothelial Growth Factor A/genetics , Vascular Endothelial Growth Factor A/metabolism , Pregnancy , Herbicides/toxicity , Chemokine CCL2/genetics , Chemokine CCL2/metabolism , AngiogenesisABSTRACT
We report a mistake in the tables published in Milesi et al. (2019).
Subject(s)
Glycine , Herbicides , Animals , Female , Glycine/analogs & derivatives , Pregnancy , Rats , Rats, Wistar , Reproduction , GlyphosateABSTRACT
The development of diabetic microangiopathy may produce lesions in distinct organic territories (the skin, among others). With an intention of identifying hemorheologic variables for a better characterization of diabetic patients, we studied plasmatic and blood viscosities (P Visc at 2.30 s(-1) and B Visc at 4.60 and 230 s(-1)), fibrinogenemia, and erythrocytic aggregation in 40 type 2 diabetic patients (13 with microangiopathic skin lesions and 27 without) and in 30 healthy controls. Considering its alterations in diabetic patients and applying linear discriminant analysis, two models may be characterized: (a) discriminant function (Disc F)=0.58 aggregate shape parameter (ASP)-0.61 B Visc(230)+0.89 fibrinogenemia for discriminating healthy individuals from diabetic patients with microangiopathic skin lesions and (b) Disc F=6.325 ASP-0.347 B Visc(230)+0.013 fibrinogenemia for discriminating healthy controls from diabetic patients with and without microangiopathic skin lesions. Both models appear to be valid due to the following: (a) Model 1: a coefficient of canonic correlation of 0.924, a highly significant Mahalanobis distance (P<10(-3)), a correct percentage of classification (100%), and the centroids of each group (0.94 and 5.63); (b) Model 2: a coefficient of canonic correlation of 0.898, a highly significant Mahalanobis distance (P<10(-3)), a correct percentage of classification (85.7%), and the centroids of each group (-1.9, 1.9, and 2.4). Just as the alterations in the analyzed hemorheologic variables could be suggesting their possible involvement in the physiopathogenia of diabetic microangiopathic skin lesions, the proposed models could characterize a microcirculatory profile in diabetic patients for preventing irreversible damages.
Subject(s)
Diabetes Mellitus, Type 2/physiopathology , Diabetic Angiopathies/classification , Diabetic Angiopathies/physiopathology , Hemorheology , Skin Diseases/physiopathology , Aged , Discriminant Analysis , Female , Humans , Male , Middle Aged , Patient Selection , Reference Values , Skin/blood supply , Skin Diseases/classification , Skin Diseases/etiologyABSTRACT
Los objetivos del trabajo fueron evaluar modificaciones del pH en la etapa de coagulación con polihidroxicloruro de aluminio, a fin de optimizar la remoción de materia orgánica y comprobar si estas modificaciones afectan la eliminación de enteroparasitos. Se trabajo con equipos de laboratorio para ensayos de coagulación. Para el recuento de enteroparasitos, se aplicaron métodos con antivuerpos monicionales por inmunofluorescencia
Subject(s)
Water Purification , Treatment Plants , Drinking Water , Coagulation (Water Treatment) , Argentina , ArgentinaABSTRACT
Los objetivos del trabajo fueron evaluar modificaciones del pH en la etapa de coagulación con polihidroxicloruro de aluminio, a fin de optimizar la remoción de materia orgánica y comprobar si estas modificaciones afectan la eliminación de enteroparasitos. Se trabajo con equipos de laboratorio para ensayos de coagulación. Para el recuento de enteroparasitos, se aplicaron métodos con antivuerpos monicionales por inmunofluorescencia
Subject(s)
Argentina , Coagulation (Water Treatment) , Treatment Plants , Water Purification , Drinking Water , ArgentinaABSTRACT
Los cambios temporales y espaciales en las concentraciones de alguno de los parametros indicativos de contaminacion en el arroyo Tapalque de la ciudad de Olavarria, reflejan que la calidad de las aguas de este recurso experimentan un deterioro luego del aporte del vuelco de la planta depuradora de liquidos cloacales que se extiende hasta el limite del partido. La planta tiene un funcionamiento normal pero no alcanza a satisfacer la demanda de la poblacion actualmente servida de la ciudad de Olavarria
Subject(s)
Argentina , Water Physicochemical Characteristics , Reference Standards , Surface WatersABSTRACT
Los cambios temporales y espaciales en las concentraciones de alguno de los parametros indicativos de contaminacion en el arroyo Tapalque de la ciudad de Olavarria, reflejan que la calidad de las aguas de este recurso experimentan un deterioro luego del aporte del vuelco de la planta depuradora de liquidos cloacales que se extiende hasta el limite del partido. La planta tiene un funcionamiento normal pero no alcanza a satisfacer la demanda de la poblacion actualmente servida de la ciudad de Olavarria
