ABSTRACT
Aerobic bacterial strains from the salt water of Lake Red (Sovata, Romania) were cultivated. More than half of the 80 strains were G(-) and formed motile straight rods. Only a few strains produced acid from D-glucose and reduced nitrate to nitrite. Optimum NaCl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. On the basis of the 16S rRNA gene sequence similarity almost half of the strains were identified as members of genus Halomonas. Other strains belonged to genera Marinobacter, Psychrobacter, Serratia, Morganella (γ-Proteobacteria), Bacillus, Exiguobacterium, Planococcus (Firmicutes), and Arthrobacter, Micrococcus, Microbacterium, and Nesterenkonia (Actinobacteria).
Subject(s)
Bacteria, Aerobic/classification , Bacteria, Aerobic/metabolism , Biodiversity , Fresh Water/microbiology , Sodium Chloride , Actinobacteria/classification , Actinobacteria/genetics , Actinobacteria/isolation & purification , Actinobacteria/metabolism , Bacteria, Aerobic/genetics , Bacteria, Aerobic/isolation & purification , Colony Count, Microbial , Culture Media , DNA, Bacterial/genetics , Fresh Water/chemistry , Gammaproteobacteria/classification , Gammaproteobacteria/genetics , Gammaproteobacteria/isolation & purification , Gammaproteobacteria/metabolism , Halomonas/classification , Halomonas/genetics , Halomonas/isolation & purification , Halomonas/metabolism , Phenotype , Phylogeny , Plankton/growth & development , RNA, Ribosomal, 16S/genetics , Romania , Sequence Analysis, DNAABSTRACT
The authors performed analysis of the prion protein gene (PRNP) in 27 out of 109 confirmed prion disease patients between 1994 and 2004. E200K mutation was found in 17 cases. Another 10 patients, lacking PRNP analysis, showed positive family history. The mean annual incidence (0.27/million) and proportion (25.6%) of genetic prion disease is unusually high in Hungary and might be related to the migration of ancestors from the Slovakian focus.
