ABSTRACT
Short-time disturbances of the microcirculation in rabbits had a different influence on the ATP-ase activity of smooth and rough microsomes of the liver and heart. Mg+2ATP-ase activity in the liver decreased in both microsome fractions; as in the heart, the enzyme activity increased only in the smooth (light) microsomes.
Subject(s)
Adenosine Triphosphatases/metabolism , Microcirculation/drug effects , Microsomes, Liver/enzymology , Microsomes/enzymology , Myocardium/enzymology , Acute Disease , Animals , Coronary Disease/enzymology , Dextrans/pharmacology , Enzyme Activation , Glucose-6-Phosphatase/metabolism , Male , Rabbits , Time Factors , Vasopressins/pharmacologyABSTRACT
Disturbance of the microcirculation produced by the combined injection of the high molecular weight dextran and vasopressin led as soon as the first minutes (5 min) to the intensification of glycolysis. This was testified to by the reduction of glycogen concentration by 19.4 percent, elevation of the phosphorylase "A" activity by 30-36 percent and of the pyruvic acid by 36.9 percent. The ATP, ADP, AMP, and the KP concentration remained unchanged. The observed glycolysis changes can be regarded as the initial metabolic reactions resulting from hypoxia originating in microcirculation disturbances.
Subject(s)
Coronary Disease/metabolism , Energy Metabolism , Myocardium/metabolism , Adenosine Diphosphate/analysis , Adenosine Monophosphate/analysis , Adenosine Triphosphate/analysis , Animals , Chinchilla , Glycogen/analysis , Lactates/analysis , Microcirculation , Myocardium/enzymology , Phosphocreatine/analysis , Phosphorus/analysis , Phosphorylases/metabolism , Pyruvates/analysis , RabbitsABSTRACT
Changes in rheological properties of the blood were produced by intravenous injection of a high-molecular weight dextran and lysin-vasopressin. The animals were decapitated in one hour. Oxygen absorption by mitochondria of the heart in oxidation of 2.5-10 mM of the succinate increased by 90-120%, as compared to control. Stimulation of respiration by ADP was decreased 1.5-2 times. Simultaneous administration of the succinate and glutamic acid normalized the respiration and phosphorylation. A possibility of inhibition of succinic-dehydrogenase by the oxalo-acetic acid was suggested. Switching of respiration to succinic acid and limiting of the SDG activity can be considered as adaptive factors under conditions of changes in rheological properties of the blood, and are directed to the maintenance of cardiac activity, this being evidenced by the absence of changes in the ATP-asic activity and in the myosin content of the heart.
