ABSTRACT
This study was conducted to investigate the presence of Epstein-Barr virus (EBV) and human papillomavirus (HPV) and the promoter methylation status of the death-associated protein kinase (DAPK) gene in high-grade intraepithelial lesions. Viral infection was analyzed using polymerase chain reaction (PCR), and promoter methylation status was evaluated using chemical modification by sodium bisulfite followed by PCR. A total of 24 samples were studied. HPV was detected in 16.6%, EBV in 16.6%, and HPV/EBV coinfection in 16.6%. No virus infection was detected in 50% of the samples studied. DAPK promoter methylation was observed in 29.2% of the analyzed samples. There was no significant correlation between DAPK methylation and viral infection. DAPK methylation was detected in 28% of HPV-positive lesions, in 28% of HPV- and EBV-positive lesions, and in 44% (3/7) of the samples without viral infection. There was no observed methylation in samples with isolated EBV infection. In DAPK unmethylated samples, HPV infection was found in 12%, EBV infection in 23%, HPV/EBV coinfection in 12%, and an absence of HPV and EBV infection in 53%. The promoter methylation of the DAPK gene is an important event during carcinogenesis and may have potential clinical application as a marker for the progression and prognosis of cancer.
Subject(s)
Alphapapillomavirus/genetics , Apoptosis Regulatory Proteins/genetics , Calcium-Calmodulin-Dependent Protein Kinases/genetics , Carcinoma, Squamous Cell/genetics , DNA Methylation , DNA, Viral/analysis , Herpesvirus 4, Human/genetics , Uterine Cervical Dysplasia/genetics , Uterine Cervical Neoplasms/genetics , Alphapapillomavirus/isolation & purification , Base Sequence , Carcinoma, Squamous Cell/epidemiology , Carcinoma, Squamous Cell/pathology , Carcinoma, Squamous Cell/virology , Death-Associated Protein Kinases , Epstein-Barr Virus Infections/epidemiology , Epstein-Barr Virus Infections/genetics , Epstein-Barr Virus Infections/virology , Female , HeLa Cells , Herpesvirus 4, Human/isolation & purification , Humans , Molecular Sequence Data , Papillomavirus Infections/epidemiology , Papillomavirus Infections/genetics , Papillomavirus Infections/virology , Prevalence , Promoter Regions, Genetic , Tumor Cells, Cultured , Uterine Cervical Neoplasms/epidemiology , Uterine Cervical Neoplasms/pathology , Uterine Cervical Neoplasms/virology , Uterine Cervical Dysplasia/epidemiology , Uterine Cervical Dysplasia/pathology , Uterine Cervical Dysplasia/virologyABSTRACT
Os autores apresentam 85 pacientes com I.U.E. operadas pela tecnica proposta por Burch. Apos descrever os criterios adotados no diagnostico da I.U.E., as indicacoes da via retropubica e os cuidados peroperatorios, apresentam suas complicacoes no pos-operatorio imediato ligadas a cirurgia adotada. As possiveis causas que contribuiram sao analisadas e medidas profilaticas sao propostas
