ABSTRACT
Optosensing chitosan-based membranes have been applied for the detection of heavy metals, especially in drinking water. The novelty of this study is based on the use of sulphated polysaccharides, in such optosensing membranes, aiming at an improved analytical performance. The sulphated polysaccharides, such as ulvan, fucoidan and chondroitin sulfate, were extracted from by-products and wastes of marine-related activities. The membranes were developed for the analysis of aluminum. The variation in the visible absorbance of the sensor membranes after the contact between the chromophore and the aluminum cation was studied. The membranes containing sulphated polysaccharides showed improved signals when compared to the chitosan-only membrane. As for the detection limits for the membranes containing ulvan, fucoidan and chondroitin sulfate, 0.17 mg L-1, 0.21 mg L-1 and 0.36 mg L-1 were obtained, respectively. The values were much lower than that obtained for the chitosan-only membrane, 0.52 mg L-1, which shows the improvement obtained from the sulphated polysaccharides. The results were obtained with the presence of CTAB in analysis solution, which forms a ternary complex with the aluminum cation and the chromophore. This resulted in an hyperchromic and batochromic shift in the absorption band. When in the presence of this surfactant, the membranes showed lower detection limits and higher selectivity.
Subject(s)
Chitosan , Chondroitin Sulfates , Aluminum , Cations , Polysaccharides/analysis , SulfatesABSTRACT
Puede ser necesaria una reflexión sobre cuál es el mejor abordaje para las enfermedades agudas concomitantes que pueden desarrollar los pacientes críticos con COVID-19. Estos requieren una sospecha diagnóstica y un tratamiento precoz, basados en el trabajo de equipos multidisciplinares. Presentamos dos casos de enfermedades concomitantes en pacientes con COVID-19. Un paciente diagnosticado de COVID-19 con buena evolución respiratoria que tras extubación presentó una colecistitis acalculosa y un paciente con neumonía por COVID-19 que presentó una sobreinfección con neumonía necrotizante, cuyo primer síntoma fue hemoptisis y fue finalmente tratado con embolización arterial por radiología intervencionista
It may be necessary a consideration about the best approach to the acute concomitant problems that critical COVID-19 patients can develop. They require a rapid diagnosis and an early treatment by a multidisciplinary team. As a result, we would like to describe two clinical cases a patient with diagnosis of COVID-19 pneumonia with good respiratory evolution that, after extubation suffered an acalculous cholecystitis and a patient with COVID-19 pneumonia that suffered an overinfection with necrotising pneumonia that presented with haemoptysis and was finally treated with arterial embolisation by the interventional radiologist's team
Subject(s)
Humans , Male , Middle Aged , Aged , Coronavirus Infections/complications , Pneumonia, Viral/complications , Pandemics , Acalculous Cholecystitis/complications , Pneumonia, Necrotizing/complications , Tomography, X-Ray Computed , Acalculous Cholecystitis/diagnostic imaging , Pneumonia, Necrotizing/diagnostic imagingABSTRACT
It may be necessary a consideration about the best approach to the acute concomitant problems that critical COVID-19 patients can develop. They require a rapid diagnosis and an early treatment by a multidisciplinary team. As a result, we would like to describe two clinical cases a patient with diagnosis of COVID-19 pneumonia with good respiratory evolution that, after extubation suffered an acalculous cholecystitis and a patient with COVID-19 pneumonia that suffered an overinfection with necrotising pneumonia that presented with haemoptysis and was finally treated with arterial embolisation by the interventional radiologist's team.
Subject(s)
COVID-19/complications , Cholecystitis/etiology , Pneumonia, Necrotizing/etiology , Aged , Critical Illness , Humans , Male , Middle AgedABSTRACT
Alternative splicing of mRNA precursors enables cells to generate different protein outputs from the same gene depending on their developmental or homeostatic status. Its deregulation is strongly linked to disease onset and progression. Current methodologies for monitoring alternative splicing demand elaborate procedures and often present difficulties in discerning between closely related isoforms, e.g. due to cross-hybridization during their detection. Herein, we report a general methodology using a Surface Plasmon Resonance (SPR) biosensor for label-free monitoring of alternative splicing events in real-time, without any cDNA synthesis or PCR amplification requirements. We applied this methodology to RNA isolated from HeLa cells for the quantification of alternatively spliced isoforms of the Fas gene, involved in cancer progression through regulation of programmed cell death. We demonstrate that our methodology is isoform-specific, with virtually no cross-hybridization, achieving limits of detection (LODs) in the picoMolar (pM) range. Similar results were obtained for the detection of the BCL-X gene mRNA isoforms. The results were independently validated by RT-qPCR, with excellent concordance in the determination of isoform ratios. The simplicity and robustness of this biosensor technology can greatly facilitate the exploration of alternative splicing biomarkers in disease diagnosis and therapy.
Subject(s)
Alternative Splicing/genetics , Biosensing Techniques/methods , RNA Isoforms/isolation & purification , Surface Plasmon Resonance/methods , DNA, Complementary/chemistry , Humans , Polymerase Chain Reaction , RNA Isoforms/geneticsABSTRACT
La caries dental es una enfermedad infecciosa, multifactorial, transmisible y de mayor prevalencia dentro de la morbilidad bucal. La medición del grado de riesgo es la base de la planificación y de la atención, ya que permite agrupar aquellos individuos , comunidades o poblaciones que tienen una mayor probabilidad de sufrir un daño. Objetivo: conocer los resultados de la aplicación de un programa de atención clínica en la reducción del nivel de riesgo biológico de caries dental en una población pediátrica. Materiales y métodos: se realizó un estudio epidemiológico evaluativo sobre una cohorte de 1002 historias clínicas de pacientes de ambos sexos, ingresados al Programa de Atención Odontopediátrico Integral del Hospital de Odontología Infantil Don Benito Quinquela Martín, durante el período de junio a diciembre de 2012. Resultados: sexo masculino: 464 (46,3 por ciento). Grupo 8-11 años: 49,8 por ciento. Indice de O´Leary: valor inicial promedio 35,87; valor final 13,06. Indice de placa: valor inicial promedio 0,46; valor final 0,18. Indice gingival: valor inicial promedio 0,30; valor final 0,09. Frecuencia de cepillado: valor promedio 2,16 veces. Momentos de azúcar: valor promedio 4.32. Conclusiones: la aplicación de un programa de atención clínica con un fuerte componente preventivo, elaborado a partir de la categorización del nivel de riesgo de caries dental, demostró una reducción en los indicadores de riesgo biológico de la población en estudio.
Subject(s)
Humans , Male , Adolescent , Female , Child, Preschool , Child , Dental Care for Children/methods , Environmental Biomarkers , Dental Caries/epidemiology , Dental Caries Susceptibility , Health Programs and Plans , Risk Factors , Age and Sex Distribution , Argentina , Dental Service, Hospital , DMF Index , Epidemiology, Descriptive , Oral Hygiene Index , Periodontal Index , Data Interpretation, Statistical , ToothbrushingABSTRACT
Two cases of spinal tuberculosis in atypical regions are presented; in the lumbosacral hinge and cranio-cervical regions respectively; focusing on the radiological appearance using Computed Tomography (CT) and Magnetic Resonance. With regard to these atypical presentations, the clinical history, epidemiology and radiology of spinal tuberculosis are reviewed.
Se presentan dos casos de tuberculosis espinal en localizaciones atípicas; la charnela lumbo-sacra y cráneo-cervical respectivamente; centrándonos en su aspecto radiológico por tomografía computarizada (TC) y resonancia magnética. A propósito de estas atípicas presentaciones se revisa la clínica, epidemiología y radiología de la tuberculosis en la columna vertebral.
Subject(s)
Humans , Male , Adult , Female , Aged, 80 and over , Magnetic Resonance Imaging , Tomography, X-Ray Computed , Tuberculosis, Spinal/diagnosisSubject(s)
Hyperpigmentation/diagnosis , Tinea Versicolor/diagnosis , Aged , Humans , Male , Tinea Versicolor/pathologyABSTRACT
OBJECTIVE: Systematic full-mouth dental examination during pregnancy is an official preventive measure recently advocated by the French Health policy. The aim of this study was firstly to evaluate the oral health related to some sociodemographic factors among pregnant women, and secondly to propose this dental examination together with the routine antenatal interview. MATERIAL AND METHOD: This cross-sectional study combined several medical questionnaires with an oral examination. It concerned all pregnant women attending their routine antenatal interview in the maternity unit of the Montpellier hospital. Socioeconomic status was assessed by Epices index. So that two groups were determined : the deprived group (D), and the non-deprived group (ND). RESULTS: Oral examination revealed that 93% of the women were suffering from at least one oral disease, 74% had a periodontal disease (9% had a periodontitis), and 74% had at least one carious tooth. The mean Epices score was 30.5 and the mean number of carious teeth was significantly higher in the group D (3.4) than in the group ND (2.35), (p=0.02). The prevalence of periodontal disease or periodontitis were not significantly different between the two groups (p=0.81 and p=0.99 respectively). After stratification on the degree of dental hygiene knowledge, it was found that knowing about an adequate dental hygiene and specific preventive measures regarding pregnancy could reduce the gap between the oral health status of the two socioeconomic groups. CONCLUSION: This study showed that performing an oral examination, at the same time than the antenatal interview, could highly improve the knowledge about dental hygiene among pregnant women and the screening of oral diseases, especially for deprived population.
Subject(s)
Mouth Diseases/epidemiology , Oral Health , Pregnancy Complications/epidemiology , Pregnancy , Adult , Cross-Sectional Studies , Dental Care/statistics & numerical data , Dental Caries/epidemiology , Dietary Sucrose/administration & dosage , Feeding Behavior , Female , France/epidemiology , Health Behavior , Health Knowledge, Attitudes, Practice , Humans , Periodontal Diseases/epidemiology , Smoking/epidemiology , Social Class , Toothbrushing/statistics & numerical data , Vulnerable Populations , Young AdultSubject(s)
Humans , Male , Adult , Hyperplasia/diagnosis , Skin Neoplasms/diagnosis , Skin Neoplasms/drug therapy , Biopsy/methods , Biopsy , Hyperplasia/microbiology , Hyperplasia/pathology , Granuloma, Plasma Cell/complications , Granuloma, Plasma Cell/diagnosis , Skin Neoplasms/complications , Skin Neoplasms/physiopathology , Achondroplasia/complications , Dermis/injuries , Dermis/pathology , Diagnosis, DifferentialABSTRACT
INTRODUCTION: Anti-NMDA receptor (NMDAR) encephalitis usually develops as a multistage syndrome with a broad differential diagnosis. PATIENTS: We report 2 patients with anti-NMDAR encephalitis and a clinical picture typical of this disorder but whose initial evaluation suggested other aetiologies. DISCUSSION: The frequent development of this disorder in young individuals presenting with psychiatric manifestations often suggests other diagnostic possibilities, most commonly viral encephalitis, psychiatric disorders, and neuroleptic malignant syndrome. In addition, several less clearly defined syndromes or descriptively reported disorders in adult and paediatric patients were likely cases of anti-NMDAR encephalitis. CONCLUSIONS: Anti-NMDAR encephalitis should be considered in young individuals with subacute presentation of psychiatric symptoms, abnormal movements, and autonomic dysfunction. The clinical and immunological characterization of this disorder has lead to the identification of new antibodies that affect memory, learning, behaviour and psychosis.
Subject(s)
Autoantibodies/immunology , Encephalitis , Receptors, N-Methyl-D-Aspartate/immunology , Adolescent , Adult , Diagnosis, Differential , Encephalitis/diagnosis , Encephalitis/immunology , Encephalitis/physiopathology , Female , HumansSubject(s)
Hamartoma/complications , Muscle, Smooth/pathology , Muscular Diseases/complications , Nevus, Pigmented/complications , Skin Neoplasms/complications , Adult , Hamartoma/diagnosis , Hamartoma/pathology , Humans , Male , Muscular Diseases/diagnosis , Muscular Diseases/pathology , Nevus, Pigmented/classification , Nevus, Pigmented/embryology , Shoulder , Skin Neoplasms/embryologySubject(s)
Humans , Male , Adult , Hamartoma/complications , Hamartoma/diagnosis , Hamartoma/pathology , Nevus/complications , Nevus/diagnosis , Nevus/pathology , Hyperpigmentation/diagnosis , Hyperpigmentation/pathology , Hypertrichosis/diagnosis , Hypertrichosis/pathology , Biopsy/classification , Biopsy/instrumentation , Biopsy/methodsABSTRACT
Introducción: La encefalitis por anticuerpos contra el receptor de NMDA (NMDAR) suele desarrollarse como un síndrome característico de evolución multifásica y diagnóstico diferencial amplio. Pacientes: Presentamos a 2 pacientes diagnosticadas de encefalitis por anticuerpos NMDAR con un cuadro clínico típico, pero que inicialmente señaló otras etiologías. Discusión:La afectación frecuente de pacientes jóvenes con manifestaciones psiquiátricas prominentes indica frecuentemente otras consideraciones diagnósticas; las más frecuentes son las encefalitis virales, los procesos psiquiátricos y el síndrome neuroléptico maligno. Varios síndromes previamente definidos de manera parcial o descriptiva en adultos y pacientes pediátricos probablemente eran casos de encefalitis anti-NMDAR.Conclusiones: La encefalitis anti-NMDAR debe considerarse en pacientes jóvenes con manifestaciones psiquiátricas subagudas, movimientos anormales y alteraciones autonómicas. La caracterización clínica e inmunológica de esta enfermedad ha llevado a la identificación de nuevos anticuerpos que afectan a procesos de memoria, aprendizaje, conducta y psicosis (AU)
Introduction: Anti-NMDA receptor (NMDAR) encephalitis usually develops as a multistage syndrome with a broad differential diagnosis.Patients: We report 2 patients with anti-NMDAR encephalitis and a clinical picture typical of this disorder but whose initial evaluation suggested other aetiologies.Discussion: The frequent development of this disorder in young individuals presenting with psychiatric manifestations often suggests other diagnostic possibilities, most commonly viral encephalitis, psychiatric disorders, and neuroleptic malignant syndrome. In addition, several less clearly defined syndromes or descriptively reported disorders in adult and paediatric patients were likely cases of anti-NMDAR encephalitis. Conclusions: Anti-NMDAR encephalitis should be considered in young individuals with subacute presentation of psychiatric symptoms, abnormal movements, and autonomic dysfunction. The clinical and immunological characterization of this disorder has lead to the identification of new antibodies that affect memory, learning, behaviour and psychosis (AU)
