ABSTRACT
The skin is the largest organ of the human body and its main function is to protect it from the external environment. It is exposed to injuries that require a rapid healing process to recover its functionality. Microorganisms inhabit the skin, which makes up the normal microbial flora, but in situations of injury they can cause infections that slow down the regeneration process. Therefore, there is a great interest in the development of alternative methods to accelerate the regeneration process and prevent infections. In this work, the efficacy of flavonoid 3-O-methylgalangine and the terpenic derivative Filifolinone and its mixtures, isolated from plants of the genus Heliotropium, on the stimulation of cell proliferation was evaluated. The results showed that the mixtures stimulated proliferation and migration in MA104 cells mainly due to the presence of Filifolinone, that together with the known antibacterial activity of 3-O-methylgalangine, opens new alternatives for the use of natural compounds in healing processes.
La piel es el oÌrgano maÌs grande del cuerpo humano y su funcioÌn principal es protegerla del entorno externo. EstaÌ expuesta a lesiones que requieren un proceso de curacioÌn raÌpido para recuperar su funcionalidad. Los microorganismos que habitan en la piel, constituyen la flora microbiana normal, pero en situaciones de lesioÌn pueden causar infecciones que retardan el proceso de regeneracioÌn. Por lo tanto, existe un gran intereÌs en el desarrollo de meÌtodos alternativos para acelerar el proceso de regeneracioÌn y prevenir infecciones. En este trabajo, se evaluoÌ la eficacia del flavonoide 3-O-metilgalangina y el derivado terpeÌnico Filifolinona y sus mezclas, aisladas de plantas del geÌnero Heliotropium, en la estimulacioÌn de la proliferacioÌn celular. Los resultados mostraron que las mezclas estimularon la proliferacioÌn y la migracioÌn en las ceÌlulas MA104 debido principalmente a la presencia de Filifolinona, que junto con la actividad antibacteriana conocida de la 3-O-metilgalangina, abre nuevas alternativas para el uso de compuestos naturales en los procesos de curacioÌn.
Subject(s)
Terpenes/pharmacology , Flavonoids/pharmacology , Heliotropium , Cell Proliferation/drug effects , Terpenes/chemistry , Wound Healing , Flavonoids/chemistry , Cell Movement/drug effects , Cell Survival/drug effects , Cells, Cultured , Epithelial Cells/drug effectsABSTRACT
In Chile Vibrio parahaemolyticus has been detected in 3 gastroenteritis outbreaks since 1998. The most recent outbreak occurred during the summer of 2005, affecting over 10,000 people of whom one died. Affected individuals presented with one or more of the following symptoms: diarrhea, nausea, vomiting, abdominal pain and/or fever. Fecal white blood cells were detected in only 6% of patients. The predominant serotype in the 3 outbreaks was the pandemic O3:K6 strain. Diagnosis was confirmed by isolation and identification of V. parahaemolyticus in stool cultures and/or by establishing an epidemiological link. V. parahaemolyticus isolates were 100% susceptible to tetracycline, ciprofloxacin and chloramphenicol, and universally resistant to ampicillin. Due to the public health impact of the 2005 outbreak, the Ministry of Health called for a National Task Force mandated to review epidemiological, clinical and microbiological features of the outbreak and to propose management guidelines.
