Genetic and multi-omic resources for Alzheimer disease and related dementia from the Knight Alzheimer Disease Research Center.

The Knight-Alzheimer Disease Research Center (Knight-ADRC) at Washington University in St. Louis has pioneered and led worldwide seminal studies that have expanded our clinical, social, pathological, and molecular understanding of Alzheimer Disease. Over more than 40 years, research volunteers have been recruited to participate in cognitive, neuropsychologic, imaging, fluid biomarkers, genomic and multi-omic studies. Tissue and longitudinal data collected to foster, facilitate, and support research on dementia and aging. The Genetics and high throughput -omics core (GHTO) have collected of more than 26,000 biological samples from 6,625 Knight-ADRC participants. Samples available include longitudinal DNA, RNA, non-fasted plasma, cerebrospinal fluid pellets, and peripheral blood mononuclear cells. The GHTO has performed deep molecular profiling (genomic, transcriptomic, epigenomic, proteomic, and metabolomic) from large number of brain (n = 2,117), CSF (n = 2,012) and blood/plasma (n = 8,265) samples with the goal of identifying novel risk and protective variants, identify novel molecular biomarkers and causal and druggable targets. Overall, the resources available at GHTO support the increase of our understanding of Alzheimer Disease.

[Helicobacter pylori detection in children with initially negative Gram, Giemsa and Wharting-Starry methods, using other histologic techniques]. / Detección de Helicobacter pylori en niños con los métodos de gram, giemsa y Warthing-Starry, inicialmente negativos con otras técnicas histológicas.

OBJECTIVE: To identify Helicobacter pylori by Gram, Giemsa and Warthing-Starry histological staining methods in biopsies of the digestive tract in children, when Helicobacter pylori identification was negative with hematoxilin and eosin. MATERIAL AND METHODS: We carried out a retrospective, cross-sectional and comparative study of 36 gastric biopsies received in the department of Anatomic-Pathology, in a period often months. All the biopsies were initially stained with routine Hematoxilin and Eosin. Of the 36 biopsies, 24 were negative to Helicobacter pylori. From paraffin blocks of these last biopsies three new histological sections were obtained to dye them with Gram, Giemsa and Warthing-Starry, and two medical pathologists performed the microscopic interpretation of these samples. RESULTS: The search for Helicobacter pylori was carried out in 24 initially negative biopsies, one was positive for Gram (4.16%) and negative for Giemsa and Warthing-Starry and four were both positive for Giemsa and Warthing-Starry (16.66%) and both were also negative for Gram. Only one biopsy was positive for the three methods. The remaining samples were negative for the three stains. CONCLUSION: The Giemsa and/or Warthing-Starry histologic techniques can be a more specific alternative for the determination of Helicobacter pylori in patients with negative digestive tract biopsies with the traditional method of hematoxilin and eosin.