Serum 90K/MAC-2BP glycoprotein levels in hepatocellular carcinoma and cirrhosis.

90K/MAC-2BP glycoprotein is a serum tumour marker, member of the scavenger receptor cysteine rich (SRCR) protein superfamily, involved in different immunological mechanisms. In the present study, we determined 90K serum levels by a sandwich enzyme immunoassay using the same monoclonal antibody in 11 chronic active hepatitis (CAH), 48 liver cirrhosis and 36 hepatocellular carcinoma (HCC). In comparison, the same samples were also tested for AFP. According to a cut-off point of 14 micrograms/mL for the 90K, established as 100% of specificity in 50 controls, we observed increasing positivities from CAH to cirrhosis and then to HCC (27%, 50% and 78%, respectively). In cirrhotic patients 90K levels were associated with the presence of anti-HCV antibodies, but not with the degree of liver compromise. Finally, 90K sensitivity was higher than AIFP in all groups of hepatic patients. However, further investigations are needed before proposing 90K as a clinical useful tumour marker in the progression from cirrhosis to HCC.

Lymphogranuloma venereum: a case report in an Italian traveller.

A 38-year-old man with a recent history of travel in India and unprotected sexual intercourse with Indian women, was admitted with painful enlarged lymphnodes predominantly in the right inguinal area. Diagnosis of lymphogranuloma venereum was made by means of a positive immunofluorescence test (Total Ig titer of 1:512) and a positive detection of chlamydial antigens by ELISA in a semen sample. He was successfully treated with ciprofloxacin. This observation emphasizes the relevance of infection due to C. trachomatis serotypes L1-L3 that may be acquired during travel in developing countries.

In vitro antimicrobial properties of azidothymidine (AZT).

In addition to the activity against a number of retroviruses, azidothymidine (AZT) has antibacterial activity against many bacteria. The effect of AZT on 224 bacterial species, including 25 strains of Salmonella spp. isolated from HIV-positive patients, was tested. AZT had no activity against all the strains of tested Gram-positive bacteria and Pseudomonas species (MIC > 128 micrograms/ml), whereas a different activity against Enterobacteriaceae (MIC range, 128 to 0.06 micrograms/ml) was found. In particular 76% of Salmonella spp. isolated from HIV-positive patients showed MICs > 1 microgram/ml, whereas similar MICs value were found in 50% of the Salmonella strains isolated from HIV-negative subjects. In addition, strains of Salmonella isolated from stools were more resistant to AZT when compared to strains isolated from blood even if this difference was not statistically significant. No correlation was found between length of therapy and Salmonella resistance to AZT in HIV-positive patients and a low incidence of Salmonella relapses in subjects treated with AZT was observed. The possibility that AZT may have an ancillary benefit in controlling some bacterial infections in AIDS patients is discussed.

Polarizing response of human polymorphonuclear cells to Vibrio cholerae.

Leucocytes have the capacity to respond to chemotactic factors by becoming morphologically and functionally polarized and this method has been found to be suitable for measurement of chemotaxis. This work evaluates the effect of whole Vibrio cholerae biotype El Tor polarization of human polymorphonuclear (PMN) cells in comparison with strains of V. cholerae NAG, Vibrio alginolyticus and Salmonella typhi. V. cholerae O1 induced, at different cell/bacteria ratios, a significant increase in the percentage of polarized cells compared with PMN cells stimulated with formylmethionyl-leucyl-phenylalanine (FMLP) and the other bacteria tested. The capacity of V. cholerae O1 to induce PMN cell polarization may play a role on the inflammatory response recently described in diarrhoea caused by V. cholerae.

Spontaneous binding of Vibrio cholerae to human leucocytes.

Various lymphocyte subpopulations have the capacity to bind different strains of Gram-negative bacteria. The capacity of a strain of Vibrio cholerae, biotype El Tor, isolated during an outbreak of cholera, to adhere to mononuclear cells isolated from peripheral blood was evaluated. V. cholerae binds to mononuclear cells in a dose-dependent manner. The binding was 76.1% at a cells/bacteria ratio of 1:200 and significantly decreased to 43.1% at a ratio of 1:1. The value of bound bacteria, a marker of the mean number of binding sites on the cell surface, decreased at lower cell/bacteria ratios. Studies on isolated cellular populations demonstrated that 51, 42 and 38%, respectively, of CD4+, CD8+ and B cells were bound by V. cholerae whereas monocytes exhibited a higher binding capacity. The data suggest that the percentage binding of V. cholerae to lymphocytes and monocytes was higher than the percentage found in previous studies with Gram-negative bacteria such as Yersinia enterocolitica, and Salmonella, but similar to Helicobacter pylori. The findings indicate that V. cholerae possesses multiple 'adhesins' such as fimbriae, flagella, haemagglutinins, lipopolysaccharides, and outer membrane proteins. The capacity to bind to blood lymphocytes may reflect the same capacity for the lymphocytes from the gastrointestinal associated lymphoid tissue. This cytoadherence may contribute to the uptake of V. cholerae from the gut and may contribute to activation of B cells and CD4+ lymphocytes.

Gastrospirillum hominis and human chronic gastritis.

Gastrospirillum hominis, a new spiral bacterium, was found in the gastric mucosa of two patients with antral chronic gastritis. These 2 cases originated from a series of 2781 consecutive gastric biopsies observed over a period of five years, with a prevalence of 0.072%. Dogs and cats may be responsible for transmission to humans but in our experience no contact with pets was documented. Detection of these organisms might provide new insight into the pathogenesis of human gastritis.

Incidence of Yersinia enterocolitica antibodies in patients with inflammatory joint diseases.

Agglutinating antibodies against Yersinia enterocolitica serotypes 0:3, 0:8 and, to a minor extent, 0:6 were found in 18 out of 93 patients with inflammatory joint diseases. Patients with undifferentiated arthritis showed the highest prevalence of antibodies against Yersinia enterocolitica. The possibility that serotypes other than 0:3 may be involved in triggering arthritis is discussed.