Functional expression of the free fatty acids receptor-1 and -4 (FFA1/GPR40 and FFA4/GPR120) in bovine endometrial cells.

Endometrial epithelial cells play a key defensive role as part of the innate immune response of cow uterus. An association between risk of acquiring infectious diseases and increased levels of free fatty acids postpartum has been suggested, and the use of omega-3 fatty acids such as docosahexaenoic acid (DHA) has been proposed as a beneficial strategy to improve immunity and fertility. The goal of our study was to demonstrate the presence of free fatty acid (FFA)-1 and 4 receptors in endometrial cells and to investigate their role on DHA interference in lipopolysaccharide (LPS)-induced inflammatory endometrial activation. We demonstrated that the bovine endometrial (BEND) cells line and bovine endometrium express both FFA1 and FFA4 receptors. FFA1 and FFA4 receptors were localized in the epithelium lining the endometrial cavity and in endometrial glands whereas in BEND cells a characteristic cell membrane localization of both receptors was observed. DHA, a FFA4 natural agonist, increased intracellular calcium mobilization in BEND cells, but the FFA1 agonists oleic and linoleic acids did not increase this response. DHA-induced intracellular calcium mobilization was inhibited by the FFA4 and FFA1 antagonists AH7614 and GW1100, respectively. DHA significantly reduced LPS-induced prostaglandin E2 (PGE2) production, but none of the antagonists reduced the effect produced by DHA. On the contrary, linoleic acid increased LPS-induced PGE2 production. In conclusion, endometrial cells express FFA4 and FFA1 receptors, and DHA induces intracellular calcium release via FFA4 and FFA1 receptors. DHA reduces PGE2, but this response was not mediated by FFA4 or FFA1 receptors.

Consumo, actitudes hacia el endeudamiento, materialismo e influencia de pares en adolescentes rurales del sur de Chile / Consumption, attitudes to the debt, materialism, and peer influence in rural teenagers from southern Chile

En la actualidad, los temas de carácter económico y financiero se han convertido en tópicos de gran importancia social, considerando los conceptos teóricos, las habilidades y actitudes que una persona debe poseer para desenvolverse de manera adecuada en una sociedad dominada por el consumo. Esta investigación tiene como principal objetivo describir las prácticas de consumo y endeudamiento informal, actitudes hacia el endeudamiento, materialismo y susceptibilidad a la influencia de los pares y en adolescentes rurales, según género y nivel socioeconómico de una comuna semi-rural de la Región de La Araucanía-Chile. El estudio se realizó en dos etapas, la primera cualitativa en la cual se realizaron entrevistas grupales y una fase cuantitativa, en la que se aplicaron instrumentos que medían cada una de las variables mencionadas; además fueron comparadas con variables sociodemográficas. Los resultados indican que las fuentes de dinero de estos adolescentes son principalmente de los padres, becas o trabajos informales. Presentan deudas informales y se caracterizan por la presencia de emocionalidad positiva vinculada al éxito de sus conductas de compra y fuertes sentimientos negativos asociados al fracaso de ello. En relación a diferencias de género, los hombres se muestran más materialistas e influenciables por sus pares que las mujeres. Los resultados del estudio reflejan los cambios sociales transversales, ligados al modelo neoliberal que han tenido que experimentar los adolescentes al insertarse en el mundo de la economía, independientemente de si provienen del mundo urbano o rural.

Due to the economic and technological globalization derived from the incorporation of the neoliberal model in Latin America and mainly in Chile, has implied the need to achieve new abilities. That is how currently economic and financial topics have been considering as social relevant issues. This points as much to the theoretical concepts as the abilities and attitudes that a person must have for his development of a suitable way in a society dominated by the consumption and in the global market. In addition, several authors suggest that consumption becomes a source for the self's construction. In this way, adolescents are being in a process of identity's definition according to their evolutionary stage, which is currently linked to consumption, they become big important subjects for the market, advertising and marketing. Thus, this research has as main objective to describe the practices of consumption and informal indebtedness, attitudes towards indebtedness, materialism and susceptibility to the influence of peers in rural adolescents by gender and socioeconomic level of a semi-rural commune of the Region of La Araucanía-Chile. The study was conducted in two stages, first a qualitative phase in which group interviews were conducted with a multiple case design and a quantitative phase with a correlation-multivariate design, where instruments were applied that measured each of the aforementioned variables; They were also compared with sociodemographic variables such as gender and socioeconomic level. The results were grouped into two phases, the first phase grouped according to three central nuclei, the first core are consumption practices, this indicates that the money sources of these adolescents are mainly from parents, scholarships or informal jobs, this is usually used in personal expenses, leisure and food. They are also characterized by considering their personal opinion, family and friends. In relation to the second core refers to the practices of indebtedness, it is characterized by the presence of informal debts, mainly with their parents, friends and family. Regarding the third core refers to the emotions related to the purchase, this is characterized by the presence of positive emotionality linked to the success of their buying behaviors and strong negative feelings associated with the failure of it. Regarding the second phase, it was found that adolescents are characterized by being more austere than hedonistic and at a gender level; men have a greater average in materialism and greater influence of their peers than women do. There are no significant differences according to Socioeconomic Level. On the other hand, there are positive correlations between materialism and peer influence and between hedonism and materialism. Finally, there are no significant differences according to ethnicity. The results show that subjects have high levels of inclusion and digital access similar to that of urban adolescents. There is also a tendency towards early debt behavior that is consistent with the national surveys' numbers. In relation to savings, the adolescents of the study shows that they give more importance to saving than urban ones, especially in the long term. In relation to the emotions related to the purchase, are a concern issue, because of how they could involve the creation of an identity based on consumption, as well as imply problems in their satisfaction with life and in their mental health. The results of the study reflect the transversal social changes linked to the neoliberal model, that adolescents have had to experience when enter to the economics world, regardless of whether they come from the urban or rural world.

Enfrentamiento de adultos mayores con sospecha de covid-19 en el servicio de urgencia

En Diciembre 2019, surge una enfermedad emergente, COVID-19, que es debida a un coronavirus (SARS-CoV-2) que empieza en Wuhan, China y que se ha propagado rápidamente a nivel mundial. La Organización Mundial de la Salud (OMS) lo declara como pandemia el 12 de Marzo del 2020. En Chile, el día 14 de Marzo se decretó fase 3 y el 16 de Marzo se pasó a fase 4. Al 24 de Marzo del 2020, en Chile existen 922 casos confirmados de COVID-19, con una mediana de edad de 34 años, correspondiendo 51 casos (6%) a adultos mayores (AM).

Life Satisfaction in Chilean University Students: An Examination of the Relation between Gender and Socioeconomic Level / Satisfacción con la Vida en Estudiantes Universitarios Chilenos: Una Revisión de la Relación entre Género y Nivel Socioeconómico

Abstract The objectives of this study were to examine the psychometric properties of the Multidimensional Life Satisfaction Scale in university students (MSLSS) (Huebner, 1998) in a sample of university students from northern, central and southern Chile, where indicators of reliability and construct validity were examined. Also, it seeks to describe the levels of life satisfaction of students and to examine differences between gender and between socioeconomic statuses. The study is quantitative with a cross-sectional descriptive-relational design. The Multidimensional Life Satisfaction Scale was applied to a sample of 306 university students in northern, central and southern Chile, as well as a questionnaire that collected sociodemographic variables. It was used confirmatory factor analysis to evaluate the model expected from the five factors of the 40-item version of the scale. The results showed the scale had adequate psychometric behavior in this type of population, where also high satisfaction levels in overall and specific domains were found, and group differences were found by gender but not by socioeconomic status.

Resumen Los objetivos de este estudio fueron evaluar las propiedades psicométricas de la Escala Multidimensional de Satisfacción con la Vida en Estudiantes (MSLSS) (Huebner, 1998) en una muestra de estudiantes universitarios de la zona norte, centro y sur de Chile, examinando indicadores de contabilidad y validez de constructo. Junto a esto, se buscó describir los niveles de satisfacción con la vida de los estudiantes y examinar diferencias por género y nivel socioeconómico. El estudio es cuantitativo con un diseño descriptivo relacional transversal. La Escala Multidimensional de Satisfacción con la Vida fue aplicada a una muestra intencionada de 306 estudiantes universitarios en el norte, centro y sur de Chile, al igual que un cuestionario que recogió variables sociodemográficas. Se utilizó el análisis factorial confirmatorio para evaluar el modelo esperado de cinco factores de la versión de 40 ítems de la escala. Los resultados demuestran que la escala tiene un comportamiento psicométrico adecuado para este tipo de población, también se encontraron altos niveles de satisfacción en los dominios generales y específicos, y diferencias en género, mas no en nivel socioeconómico.

Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: presence and sensitivity of 5' AMP-activated protein kinase (AMPK).

This study evaluated the effect of the use of hypometabolic TRIS extenders in the presence or the absence of AMPK activators as well as the utilization of high cooling rates in the refrigeration step on the freezability of stallion sperm. Twelve ejaculates were cryopreserved using Botucrio® as a control extender and a basic TRIS extender (HM-0) separately supplemented with 10 mM metformin, 2mM 5-aminoimidazole-4-carboxamide-1-ß-D-ribofuranoside (AICAR), 2 mM Adenosine monophosphate (AMP), 40 µM compound C AMPK inhibitor or 2 mM AMP+40 µM compound C. Our results showed that the utilization of a hypometabolic TRIS extender supplemented or not with AMP or metformin significantly improves stallion sperm freezability when compared with a commercial extender. Additionally, high cooling rates do not affect stallion sperm quality after cooling and post-thawing. Finally, stallion spermatozoa present several putative AMPK sperm isoforms that do not seem to respond to classical activators, but do respond to the Compound C inhibitor.