ABSTRACT
HIGHLIGHTS: The aflatoxin M1 content in milk was not related to the enrichment factor. The enrichment factor in 45-day ripened semihard cheese was defined. The enrichment factor in cheese is affected by cheese yield.
Subject(s)
Aflatoxin M1 , Cheese , Food Microbiology , Milk , Aflatoxin M1/analysis , Animals , Cattle , Cheese/analysis , Cheese/microbiology , Cheese/standards , Milk/chemistry , Milk/microbiologyABSTRACT
BACKGROUND: Due to very sluggish turnover at the molecular and cellular level, the healing of chondral damages has been considered difficult. In the current study, the effects of the Kartogenin, a small heterocyclic molecule on chondrogenic differentiation of stem cells was compared to TGF-ß3. METHODS: Human Adipose-Derived Stem Cells were extracted during an elective surgery. Cell viability was estimated by MTT assay, differentiated cells evaluated by histological and immunohistochemical techniques. Expression of cartilage specific genes (SOX9, Aggrecan, type II and X collagens) assessed by real-time PCR. RESULTS: The real-time PCR assay has revealed the expression of gene marker of chondrogenesis, SOX9, Aggrecan and type II collagen, both in Kartogenin and TGFß3 groups compared to the control group, significantly (p < 0.05). A low expression level of collagen type X as a hypertrophic marker was seen in cartilage produced by using Kartogenin. Meanwhile, the level of type X collagen protein in Kartogenin group was significantly decreased (p > 0.05) compared to TGF-ß3 group. CONCLUSION: Kartogenin was suitable for successful chondrogenic differentiation of human adipose- derived stem cells and a suppressor of the consequent hypertrophy (Tab. 1, Fig. 5, Ref. 31).
Subject(s)
Anilides/pharmacology , Chondrogenesis/drug effects , Phthalic Acids/pharmacology , Stem Cells/drug effects , Transforming Growth Factor beta3/pharmacology , Adipose Tissue/cytology , Aggrecans/drug effects , Aggrecans/genetics , Cartilage , Cell Differentiation/drug effects , Cells, Cultured , Collagen Type II/drug effects , Collagen Type II/genetics , Collagen Type X/drug effects , Collagen Type X/genetics , Fibrin , Humans , Real-Time Polymerase Chain Reaction , SOX9 Transcription Factor/drug effects , SOX9 Transcription Factor/genetics , Tissue ScaffoldsABSTRACT
The Chianina, one of the oldest and most important cattle breeds of Italy, is now reared all over the world. The Chianina has been known and appreciated since ancient times because, from a nutritional point of view, its meat has no proper rivals. To date, studies have been performed to evaluate the genetic profile of the breed, but knowledge about the chemical profile is generally lacking. Due to the increased interest from farmers regarding breeding of the Chianina, this study proposes a preliminary evaluation of main endogenous urinary corticosteroids (cortisol and cortisone) and most commonly used synthetic one (dexamethasone). Moreover, after recent findings regarding the presence of endogenous prednisolone in the urine of more popular breeds, particular attention was given to analysis of the presence of prednisolone and prednisone, as well. For this aim, the urine samples of 12 young cows and 30 young bulls was collected at the farms and analysed using a fit-for-purpose LC-MS/MS method. The preliminary results of this study show that prednisolone was found only in Chianina females (3 out of 12). Cortisol and cortisone were found at concentrations that showed a high inter-individual variability, and that were higher in female urine compared to that of males.
Subject(s)
Cattle/physiology , Cortisone/urine , Hydrocortisone/urine , Prednisolone/urine , Animals , Cattle/genetics , Cattle/urine , Chromatography, Liquid , Cortisone/chemistry , Female , Hydrocortisone/chemistry , Male , Molecular Structure , Prednisolone/chemistry , Tandem Mass SpectrometryABSTRACT
BACKGROUND: Breast cancer cells over-express the adenosine receptor A1 and in most of these cells, P53 gene is a wild type. Because of this finding and relationship between A1 receptor and cell apoptosis and proliferation, this study aimed to determine the effect of agonist and antagonist of A1 receptor on cell apoptosis and proliferation and recognize the relationship between this receptor and P53 expression. METHODS: We used a Real-Time PCR test for measuring expression of p53 gene also flow cytometry assay for apoptotic and survival cell rate after treatment of MCF-7 cells with A1 receptor agonist CPA (N6-Cyclopentyladenosine) and A1 receptor antagonist DPCPX (1,3-dipropyl-8-cyclopentylxanthine) in 24,48 and 72 hours. RESULTS: Our flow cytometry findings indicate that DPCPX significantly induces apoptosis in MCF-7. Also the expression of P53 becomes upregulated with time of DPCPX treatment. CPA treatment increased the survival cell rate and down-regulated this apoptosis-relevant gene P53 (p > 0.05). CONCLUSION: DPCPX can induce P53 expression which consequently promotes the cell apoptosis in MCF-7. Therefore, DPCPX could be used as an anti-cancer agent (Tab. 1, Fig. 3, Ref. 5).
Subject(s)
Adenosine/analogs & derivatives , Breast Neoplasms , Receptor, Adenosine A1/metabolism , Xanthines/pharmacology , Adenosine/pharmacology , Adenosine A1 Receptor Agonists/pharmacology , Adenosine A1 Receptor Antagonists/pharmacology , Apoptosis/drug effects , Cell Proliferation/drug effects , Cell Survival/drug effects , Gene Expression Profiling , Genes, p53/genetics , Humans , MCF-7 CellsABSTRACT
BACKGROUND: Carbon nanotubes (CNTs) have a large variety of applications in tissue engineering and biomedical devices. The biocompatibility and cytotoxicity of CNTs have been studied widely, however, up until now; there was uncertainty on how nanosized materials behave in the human body and stem cells. The current study describes the functionalized carbon nanotubes on adipose-derived stem cells (ADSCs) for viability and proliferation purposes in vitro. MATERIALS AND METHODS: After chemical modification of the CNTs, the ADSCs were cultured in Dulbecco's Modified Eagle's. Medium (DMEM) having doses of 0.1, 1, 10, 20, 50, and 100 µg/ml of CNTs. On the third and seventh days of the experiment, the cellular viability, proliferation, and stemness were determined, using the MTT, trypan Blue, and flow cytometry assays in variable CNTs dosage. RESULTS: In doses of 0.1 and 1 µg/ml, the expression of the surface markers were similar to the control groups on day three, but decreased in higher dosages on day seven. The viability of both groups was the same on day three, but in comparison to the control groups, was found to decrease in the higher dosages on day seven. CONCLUSION: The effect of CNTs on the viability and proliferation of ADSCs is a function of time and the doses used. Through further investigation by using these particles, we expect that we should be able to increase the viability and proliferation of ADSCs.
ABSTRACT
A case of pericardial effusion presenting clinically with pretamponade is shown. TBC is not a rare cause of Pericardial effusion and at present Tuberculosis is a more and more frequent infection also in occidental countries. Guide lines for diagnosis and treatment are revised.
Subject(s)
Abdominal Pain/microbiology , Anorexia/microbiology , Fever/microbiology , Pericardial Effusion/complications , Pericardial Effusion/diagnosis , Pericarditis, Tuberculous/complications , Pericarditis, Tuberculous/diagnosis , Aged , Cardiac Tamponade/complications , Cardiac Tamponade/diagnosis , Cardiac Tamponade/microbiology , Diagnosis, Differential , Echocardiography , Electrocardiography , Humans , Male , Pericardial Effusion/microbiology , Pericarditis, Constrictive/complications , Pericarditis, Constrictive/diagnosisABSTRACT
A severe, life-threatening metabolic alchalosis associated with a stenosing pancreatic carcinoma in a female type II diabetic patients is presented, and a review of the most frequent causes of hyperemesis, orthostatic hypotension and lethargy is shown.
