ABSTRACT
Somatic embryos (SE) of habanero pepper (Capsicum chinense Jacq.) represent persistent deformations in the shoot apical meristem (SAM), which inhibits their capacity to form organs and subsequently plants. In dicotyledonous plants, SAM is formed in the apex, between cotyledons and it plays a central role in postembryonic shoot organ formation. Based on the previous knowledge on the role of some families of gene in the formation, organization and maintenance of the SAM, the expression patterns of WUS, WOX2, NAM, STM, PIN1 and PIN7 genes were analysed, which would allow us to elucidate the possible implication of these genes in SAM deformations in the SE of C. chinense. The results show that the expression patterns of STM and PIN1 in the SE were completely opposite to the respective expression pattern obtained in zygotic embryos (ZE). Moreover, NAM and PIN7 showed an over accumulation of transcripts in SE, compared with ZE. This is the first time in the genus Capsicum that alterations in the expression pattern of key genes of the SE development are reported, as well as its possible implication in the persistent deformations of the SAM.
Subject(s)
Capsicum/genetics , Meristem/embryology , Meristem/genetics , Plant Proteins/genetics , Capsicum/embryology , Cotyledon/embryology , Cotyledon/genetics , Gene Expression Regulation, Plant , Homeodomain Proteins/metabolism , Meristem/growth & development , Microfilament Proteins/metabolism , NIMA-Interacting Peptidylprolyl Isomerase/metabolism , Plant Somatic Embryogenesis Techniques , Repressor Proteins/metabolismABSTRACT
In recent years, halotolerant biofilms have become a subject of interest for its application in Bioelectrochemical systems for wastewater treatment. To determine if the polarization potential affects the microbial community of a halotolerant bioanode, four bioanodes were poised at potentials of +0.34â¯V/SHE andâ¯-â¯0.16â¯V/SHE and the 16S rRNA gene was analyzed through a MiSeq (Ilumina) system. Oceanospirillum, Halomonas and Marinobacterium were the most predominant genus; no previous studies have reported the presence of Oceanospirillum in anodic biofilms. The fitness with the dataset for +0.34â¯V/SHE with a modified Butler Volmer Monod model, gives a value of K1 was 0.0002 (2.64 A m-2 and 38% coulombic efficiency), indicating the fastest electrochemical reaction. Whereas that -0.16â¯V/SHE case, the high value of K1 (12.2 with 1.82 A m-2 and 10% coulombic efficiency) indicated that the electron transfer was far from being reversible (Nernstian).
