Limited efficacy of a commercial microbial inoculant for improving growth and physiological performance of native plant species.

Soil microbial inoculants are increasingly being explored as means to improve soil conditions to facilitate ecological restoration. In southwestern Western Australia, highly biodiverse Banksia woodland plant communities are increasingly threatened by various factors including climate change, land development and mining. Banksia woodland restoration is necessary to conserve this plant community. The use of microbial inoculation in Banksia woodland restoration has not yet been investigated. Here, we evaluated the efficacy of a commercial microbial inoculant (GOGO Juice, Neutrog Australia Pty Ltd) for improving the performance of 10 ecologically diverse Banksia woodland plant species in a pot experiment. Plants were subjected to one of two watering regimes (well-watered and drought) in combination with microbial inoculation treatments (non-inoculated and inoculated). Plants were maintained under these two watering treatments for 10 weeks, at which point plants in all treatments were subjected to a final drought period lasting 8 weeks. Plant performance was evaluated by plant biomass and allocation, gas exchange parameters, foliar carbon and nitrogen and stable isotope (δ15N and δ13C) compositions. Plant xylem sap phytohormones were analysed to investigate the effect of microbial inoculation on plant phytohormone profiles and potential relationships with other observed physiological parameters. Across all investigated plant species, inoculation treatments had small effects on plant growth. Further analysis within each species revealed that inoculation treatments did not result in significant biomass gain under well-watered or drought-stressed conditions, and effects on nitrogen nutrition and photosynthesis were variable and minimal. This suggests that the selected commercial microbial inoculant had limited benefits for the tested plant species. Further investigations on the compatibility between the microorganisms (present in the inoculant) and plants, timing of inoculation, viability of the microorganisms and concentration(s) required to achieve effectiveness, under controlled conditions, and field trials are required to test the feasibility and efficacy in actual restoration environments.

Nitrogen deposition suppresses ephemeral post-fire plant diversity.

Fire is a dominant force shaping patterns of plant diversity in Mediterranean-type ecosystems. In these biodiversity hotspots, including California's endangered coastal scrub, many species remain hidden belowground as seeds and bulbs, only to emerge and flower when sufficient rainfall occurs after wildfire. The unique adaptations possessed by these species enable survival during prolonged periods of unfavorable conditions, but their continued persistence could be threatened by nonnative plant invasion and environmental change. Furthermore, their fleeting presence aboveground makes evaluating these threats in situ a challenge. For example, nitrogen (N) deposition resulting from air pollution is a well-recognized threat to plant diversity worldwide but impacts on fire-following species are not well understood. We experimentally evaluated the impact of N deposition on post-fire vegetation cover and richness for three years in stands of coastal sage scrub that had recently burned in a large wildfire in southern California. We installed plots receiving four levels of N addition that corresponded to the range of N deposition rates in the region. We assessed the impact of pre-fire invasion status on vegetation dynamics by including plots in areas that had previously been invaded by nonnative grasses, as well as adjacent uninvaded areas. We found that N addition reduced native forb cover in the second year post-fire while increasing the abundance of nonnative forbs. As is typical in fire-prone ecosystems, species richness declined over the three years of the study. However, N addition hastened this process, and native forb richness was severely reduced under high N availability, especially in previously invaded shrublands. An indicator species analysis also revealed that six functionally and taxonomically diverse forb species were especially sensitive to N addition. Our results highlight a new potential mechanism for the depletion of native species through the suppression of ephemeral post-fire bloom events.

Functional traits and drought strategy predict leaf thermal tolerance.

Heat stress imposes an important physiological constraint on native plant species-one that will only worsen with human-caused climate change. Indeed, rising temperatures have already contributed to large-scale plant mortality events across the globe. These impacts may be especially severe in cities, where the urban heat island effect amplifies climate warming. Understanding how plant species will respond physiologically to rising temperatures and how these responses differ among plant functional groups is critical for predicting future biodiversity scenarios and making informed land management decisions. In this study, we evaluated the effects of elevated temperatures on a functionally and taxonomically diverse group of woody native plant species in a restored urban nature preserve in southern California using measurements of chlorophyll fluorescence as an indicator of leaf thermotolerance. Our aim was to determine if species' traits and drought strategies could serve as useful predictors of thermotolerance. We found that leaf thermotolerance differed among species with contrasting drought strategies, and several leaf-level functional traits were significant predictors of thermotolerance thresholds. Drought deciduous species with high specific leaf area, high rates of transpiration and low water use efficiency were the most susceptible to heat damage, while evergreen species with sclerophyllous leaves, high relative water content and high water use efficiency maintained photosynthetic function at higher temperatures. While these native shrubs and trees are physiologically equipped to withstand relatively high temperatures in this Mediterranean-type climate, hotter conditions imposed by climate change and urbanization may exceed the tolerance thresholds of many species. We show that leaf functional traits and plant drought strategies may serve as useful indicators of species' vulnerabilities to climate change, and this information can be used to guide restoration and conservation in a warmer world.

Cultivating scientific literacy and a sense of place through course-based urban ecology research.

Undergraduate research experiences have been shown to increase engagement, improve learning outcomes, and enhance career development for students in ecology. However, these opportunities may not be accessible to all students, and incorporating inquiry-based research directly into undergraduate curricula may help overcome barriers to participation and improve representation and inclusion in the discipline. The shift to online instruction during the COVID-19 pandemic has imposed even greater challenges for providing students with authentic research experiences, but the pandemic may also provide a unique opportunity for creative projects conducted remotely. In this paper, I describe a course-based undergraduate research experience (CURE) designed for an upper-level ecology course at California State University, Dominguez Hills during remote learning. The primary focus of student-led research activities was to explore the potential impacts of the depopulation of campus during the pandemic on urban coyotes (Canis latrans), for which there were increased sightings reported during this time. Students conducted two research studies, including an evaluation of urban wildlife activity, behavior, and diversity using camera traps installed throughout campus and analysis of coyote diet using data from scat dissections. Students used the data they generated and information from literature reviews, class discussions, and meetings with experts to develop a coyote monitoring and management plan for our campus and create posters to educate the public. Using the campus as a living laboratory, I aimed to engage students in meaningful research while cultivating a sense of place, despite being online. Students' research outcomes and responses to pre- and post-course surveys highlight the benefits of projects that are anchored in place-based education and emphasize the importance of ecological research for solving real-world problems. CUREs focused on local urban ecosystems may be a powerful way for instructors to activate ecological knowledge and capitalize on the cultural strengths of students at urban universities.

Invasive annuals respond more negatively to drought than native species.

In his foundational list of 'ideal weed' characteristics, Baker (1965) proposed that weedy plants maximize reproductive output under high resource availability. Since then, the idea that invasive plant species are more responsive to fluctuating resources compared with native or noninvasive species has gained considerable traction, although few studies extend this hypothesis to include reproductive output. We revisit Baker's hypothesis in the context of invasion and drought in California grasslands, exploring whether invasives show greater growth and reproductive responses to water availability compared with the native wildflowers they displace. In an outdoor potted study, we grew eight native and eight invasive species of annuals commonly found in southern California grasslands to reproductive maturity under both well-watered and drought conditions. While drought negatively impacted plant performance overall, invasives showed more negative responses for growth and reproductive traits. Invasives also grew larger than native species, especially under well-watered conditions, and produced seed with higher rates of germination. Invasives may be more negatively impacted by drought compared with natives, but they are also able to capitalize on high resource conditions and greatly increase reproductive output. Such opportunistic responses exhibited by invasives might explain previously observed fluctuations in their abundance under variable precipitation.

Can we condition native plants to increase drought tolerance and improve restoration success?

A common method in ecological restoration is the transplanting of nursery-grown seedlings to the field and, with proper resources, this technique can be highly successful. However, stressors such as drought may negatively impact plant performance and restoration success, especially in dryland ecosystems. Furthermore, increasing environmental change may hamper the ability of practitioners to restore native vegetation. A growing body of research suggests that exposing plants to a stressor may improve tolerance to subsequent stress events later in life. We sought to understand if such a phenomenon could be exploited in order to improve plant drought-tolerance and aid native plant restoration in southern California. In a multi-phase experiment, we first exposed seedlings of native perennials to episodic drought and then later compared the response of these plants to a second drought event to that of well-watered controls. We also transplanted replicates of both treatments to a restoration site in the field to test whether exposure to drought as a seedling could improve plant performance. Plant species responded to drought differently, with species exhibiting the full range of positive, neutral, and negative responses to temporal variability in water stress. However, some species appeared to benefit from drought preconditioning, exhibiting greater growth and increased water-use efficiency compared to well-watered plants. This suggests that simple applications of stress treatments could improve plant growth and stress tolerance, but the success of this method is likely very species specific. Restoration practitioners should consider conducting pilot studies with target plant species to better understand if this technique could assist in achieving restoration goals.

High N, dry: Experimental nitrogen deposition exacerbates native shrub loss and nonnative plant invasion during extreme drought.

Hotter, longer, and more frequent global change-type drought events may profoundly impact terrestrial ecosystems by triggering widespread vegetation mortality. However, severe drought is only one component of global change, and ecological effects of drought may be compounded by other drivers, such as anthropogenic nitrogen (N) deposition and nonnative plant invasion. Elevated N deposition, for example, may reduce drought tolerance through increased plant productivity, thereby contributing to drought-induced mortality. High N availability also often favors invasive, nonnative plant species, and the loss of woody vegetation due to drought may create a window of opportunity for these invaders. We investigated the effects of multiple levels of simulated N deposition on a Mediterranean-type shrubland plant community in southern California from 2011 to 2016, a period coinciding with an extreme, multiyear drought in the region. We hypothesized that N addition would increase native shrub productivity, but that this would increase susceptibility to drought and result in increased shrub loss over time. We also predicted that N addition would favor nonnatives, especially annual grasses, leading to higher biomass and cover of these species. Consistent with these hypotheses, we found that high N availability increased native shrub canopy loss and mortality, likely due to the higher productivity and leaf area and reduced water-use efficiency we observed in shrubs subject to N addition. As native shrub cover declined, we also observed a concomitant increase in cover and biomass of nonnative annuals, particularly under high levels of experimental N deposition. Together, these results suggest that the impacts of extended drought on shrubland ecosystems may be more severe under elevated N deposition, potentially contributing to the widespread loss of native woody species and vegetation-type conversion.