ABSTRACT
2,000 microbial strains were isolated from soil samples and tested to determine their lipolytic activity by employing screening techniques on solid and in liquid media. Culture broths were initially tested with 1,2-O-dilauryl-rac-glycero-3-glutaric acid-resorufinyl ester, a chromogenic substrate specific for lipases. Fourteen lipase-producing microorganisms were selected and their taxonomic identification was carried out. Hydrolysis of tributyrin or olive oil and the esterification of oleic acid with heptanol were selected to preliminary evaluate the catalytic activity of these lipases. All the selected lipases catalysed this esterification reaction with good yields. Resolution of (R,S)-2-(4-isobutylphenyl) propionic acid, (R,S)-1-phenylethanol, (R,S) 1-phenylethylamine and of (R) or (S) glycidol were performed to evaluate the stereoselectivity of these novel enzymes as biocatalysts in reactions in organic media. Lipases from the fungi Fusarium oxysporum and Ovadendron sulphureo-ochraceum gave the best yields and enantioselectivities in the resolution of racemic ibuprofen and 1-phenylethanol. Several lipases displayed a high stereoselectivity in the resolution of chiral amines by an alcoxycarbonylation reaction.
ABSTRACT
Actinomycosis of the cervicofacial area is a rare infection with a difficult clinical diagnosis. We report a case of lingual actinomycosis which was diagnosed after a long remission with empirical antibiotic treatment, two years after onset. The clinical characteristics, differential diagnosis, and therapeutic options in this unusual infection are discussed.
Subject(s)
Actinomycosis/pathology , Tongue Diseases/pathology , Actinomycosis/drug therapy , Actinomycosis/surgery , Adult , Anti-Bacterial Agents/therapeutic use , Clindamycin/therapeutic use , Diagnosis, Differential , Gentamicins/therapeutic use , Humans , Injections, Intravenous , Magnetic Resonance Imaging , Male , Netilmicin/therapeutic use , Tongue Diseases/drug therapy , Tongue Diseases/surgeryABSTRACT
An interesting case of laryngeal herpetic involvement simulating a neoplasm in its gross appearance and the production of paralysis is reported. The patient was diagnosed as thymoma three months later. The possibilities of differential diagnosis and relation with immunosuppression states are discussed.
Subject(s)
Herpesviridae Infections/virology , Laryngeal Diseases/virology , Aged , Biopsy , Herpesviridae Infections/pathology , Herpesviridae Infections/therapy , Humans , Laryngeal Diseases/pathology , Laryngeal Diseases/therapy , Larynx/pathology , MaleABSTRACT
Chrysosporium undulatum sp. nov., showing intermediate characteristics between Chrysosporium and Malbranchea, is described and illustrated. This taxon is characterised by long and curved to loosely coiled hyphal appendages, deflected to wavy fertile hyphae forming dense tufts and small, subglobose, frequently intercalary, verrucose conidia. Restriction enzyme analysis and comparison of sequences of the ITS1-5.8S-ITS2 ribosomal DNA region of the most similar species strongly support this proposal.
