ABSTRACT
To assess the carcinogenic response of duck hepatic tissue, an experimental study was undertaken. Pure aflatoxin B1, was administered to twelve white pekin ducks of two to three months of age at a dose rate of 0.04165 mg/kg body weight every third day for six months. There was reduction in body weight and haemoglobin level from the third month onwards. Total serum protein, albumin and globulin had a slow and gradual reduction and ESR was significantly increased from the third month. Serum enzymes (AST, ALT, GGT and ALP) were significantly increased from the Vlllth to XVth fortnights (Two weeks). Ten ducks developed hepatic tumours by 180th day. Four of them had neoplastic nodules on the 90th day. Histo-pathologically they were hepatocellular carcinoma (6), Cholangiocellular carcinoma (4) and Chronic hepatitis (2). There was moderate to severe expression of GGT and ALP in the liver tissue during neoplastic transformation.
ABSTRACT
A herpesvirus was isolated from tumours of the ethmoidal mucosa in two of three head of cattle in the State of Kerala, India. The virus designated M40 was cytopathic for a variety of cultured bovine and porcine cells and it did not kill suckling mice or chicken embryos. Sera from tumour-bearing cattle and goats reacted with the M40 virus. Immunofluorescence tests with FITC-conjugated IgG from a bovine monospecific antiserum to bovine herpesvirus 4 (BHV-4) stained the M40 virus specific antigen in infected cells. Experimental infection of goats with the M40 virus did not result in development of tumours. This virus is therefore considered to represent a "passenger" virus. A great similarity was found between restriction patterns of DNAs extracted from M40 virus and the strain 66-P-347, a reference strain of the BHV-4 group.
Subject(s)
Cattle Diseases/microbiology , Ethmoid Sinus , Herpesviridae Infections/veterinary , Herpesviridae/classification , Paranasal Sinus Neoplasms/veterinary , Animals , Cattle , Chick Embryo , Goats , Herpesviridae Infections/microbiology , Mice , Paranasal Sinus Neoplasms/microbiologyABSTRACT
The distribution and ultrastructure of mast cells in the duck were studied. Carnoy's fluid was found to be the best fixative, and Padawer's method was found to be useful in demonstrating the metachromasia. Mast cells were seen in many of the organs and were most numerous in the proventriculus. Perivascular location was frequently seen. The ultrastructure resembled that of the chicken mast cell. The cell contained different types of membrane-bound granules, some of which were electron-dense and mottled.
Subject(s)
Ducks/anatomy & histology , Mast Cells/ultrastructure , Animals , FemaleABSTRACT
An experiment was carried out to measure the cell mediated immune response in pigs by sensitising the animals with 2,4-dinitrochlorobenzene (DNCB). The pigs could be sensitised with one application of DNCB. The appropriate site for application and the sensitising and challenge dose were standardised. The histological response was characteristic of a delayed hypersensitivity reaction.
