ABSTRACT
The effect of some gelatin plasma substitutes (Haemaccel, plasmagel and Plasmion (Geloplasma), which are widely used in Europe) on collagen-, ADP- and adrenaline-induced platelet aggregation in human PRP in vitro was studied under controlled conditions (pH, electrolyte composition). Haemaccel inhibited these aggregations, both in citrated as well as in heparinised PRP, whereas they were enhanced by both Plasmagel and Plasmion as compared to the appropriate control. Increasing teh concentration of the inducer overcame the inhibition by Haemaccel. Haemaccel inhibited, while Plasmion enhanced 14C-serotonin release induced by collagen, ADP or adrenaline. Also in the presence of indomethacin (90 muM) Haemaccel inhibited aggregation induced by high concentrations of collagen and the primary aggregation induced by ADP and adrenaline, while Plasmion enhanced these aggregations induced by ADP and adrenaline, while Plasmion enhanced these aggregations. The inhibition by Haemaccel was not caused by binding of Ca2+ to haemaccel.
Subject(s)
Gelatin/analogs & derivatives , Gelatin/pharmacology , Plasma Substitutes/pharmacology , Platelet Aggregation/drug effects , Adenosine Diphosphate/pharmacology , Calcium/pharmacology , Citrates/pharmacology , Collagen/pharmacology , Epinephrine/pharmacology , Heparin/pharmacology , Humans , Indomethacin/pharmacology , Serotonin/metabolismABSTRACT
Acquired factor VIII deficiency in women postpartum due to a factor VIII inhibitor is rare and the etiology is unknown. In this study a case report and a review of the literature are given. The haemorrhagic diathesis resembles classic haemophilia, with the exception that ecchymoses and tissue bleeding occur more frequently. The potency of the inhibitor may vary from weak to strong and the inactivation of factor VIII coagulant activity (factor VIII-C) by the inhibitor is of a non-linear type. Severe bleeding has been fatal in a few cases, but factor VIII concentrate substitution has usually been successful without anamnestic response of inhibitor activity. There is no convincing evidence that immunosuppression is effective, also because the natural history of the disease is characterised by a spontaneous disappearance of the factor VIII-C inhibitor. Treatment of bleeding symptoms with factor VIII concentrate should therefore not be reserved for life threatening haemorrhages only.
Subject(s)
Factor VIII/antagonists & inhibitors , Hemophilia A/blood , Postpartum Hemorrhage/blood , Adult , Cryoglobulins/therapeutic use , Female , Hemophilia A/drug therapy , Humans , Postpartum Hemorrhage/drug therapy , Pregnancy , Time FactorsABSTRACT
A rapid and reliable gas-chromatographic method is described for the estimation of phenprocoumon from human serum or plasma. No interference was found from other usual oral anticoagulant drugs. The method can be used for therapeutic and toxic levels. The appearance of two volatile derivatives of phenprocoumon (in constant ratio) is discussed.
Subject(s)
4-Hydroxycoumarins/blood , Phenprocoumon/blood , Chromatography, Gas/methods , Humans , Microchemistry , PlasmaABSTRACT
The recovery of factor VII-activity in cryoprecipitate prepared from plasma from six patients with von Willebrand's disease was low as compared to the recovery from NORMAL ANd hemophilia-A plasma. The possible cause is briefly discussed.
Subject(s)
Factor VIII/analysis , von Willebrand Diseases/blood , Antigens , Chemical Precipitation , Cold Temperature , Factor VII/immunology , Hemophilia A , HumansSubject(s)
Anti-Bacterial Agents/pharmacology , Blood Coagulation/drug effects , Fatty Acids, Unsaturated/pharmacology , Absorption , Blood Coagulation Tests , Calcium/metabolism , Cholesterol , Factor IX/metabolism , Factor VIII/metabolism , Factor X/metabolism , Humans , Inositol , Lactones/pharmacology , Lipid Metabolism , Polyenes/pharmacology , Thrombin/metabolismABSTRACT
Ristocetin-induced platelet aggregation was increased after exercise in 5 healthy volunteers. It was shown that this was the result of the increase of a plasmatic factor.
