ABSTRACT
A simple and reproducible method for the preparation and labeling of leukocytes with Tc-99m has been developed. Leukocytes were separated from blood, incubated with stannous pyrophosphate, and then with 20-30 mCi (740-1110 MBq) of 99mTcO-4. In leukocytes separated from human blood, the labeling efficiency was 81% +/- 6% (n = 4). Experiments on dogs with abscesses showed accumulation of the Tc-99m-labeled leukocytes in the infected sites, indicating the viability of the labeled leukocytes. Additional studies showed that rat lymphocytes that were labeled with Tc-99m, using the same technique, localized in heart transplant tissue that was being rejected.
Subject(s)
Abscess/diagnostic imaging , Graft Rejection , Leukocytes , Technetium , Abscess/immunology , Animals , Dogs , Heart Transplantation , Humans , Isotope Labeling , Leukocytes/immunology , Leukocytes/ultrastructure , Lymphocytes/immunology , Microscopy, Electron , Myocardium/immunology , Radionuclide Imaging , Rats , Sodium Pertechnetate Tc 99mABSTRACT
Localization of acute myocardial infarcts can be done directly by imaging 99mTc pyrophosphate in the infarcted myocardium, or indirectly by analysis of wall motion abnormalities. Combining these modalities improves the sensitivity and specificity of the study. Forth-nine consecutive abnormal studies were analyzed in which both wall motion and static images were obtained with a single injection of pyrophosphate. At least 50% of the acute infarcts were better localized (or additional information obtained) by combining the modalities.
Subject(s)
Diphosphates , Heart Ventricles/physiopathology , Myocardial Infarction/diagnosis , Radionuclide Imaging , Technetium , Adult , Aged , Female , Humans , Male , Middle Aged , Myocardial Infarction/physiopathologyABSTRACT
There were 98 patients with a variety of intrascrotal lesions studied with a radioisotope 99mtechnetium-pertechnetate scanning technique. Retrospective analysis in 50 patients subjected to an operation revealed a 94 per cent accuracy of the scan in the differential diagnosis of testicular torsion and epididymitis as compared to a clinical accuracy of 48 per cent. In 7 patients with a scan diagnosis of epididymo-orchitis with abscess the diagnosis was confirmed during the operation or by followup examination. While the scan has been a simple, safe, rapid and reliable technique to differentiate acute and subacute lesions, it has proved to be of limited diagnostic significance in cases of chronic intrascrotal lesions and carcinoma.
