ABSTRACT
Positron emission tomography with O-15-labeled water was used to study at rest the neurophysiological effects of bilateral external globus pallidus (GPe) deep brain stimulation in patients with Huntington's disease (HD). Five patients were compared with a control group in the on and off states of the stimulator. External globus pallidus stimulation decreased neuronal activity and modulated cerebral connectivity within the basal ganglia-thalamocortical circuitry, the sensorimotor, and the default-mode networks. These data indicate that GPe stimulation modulates functional integration in HD patients in accordance with the basal ganglia-thalamocortical circuit model.
Subject(s)
Deep Brain Stimulation , Globus Pallidus/physiology , Huntington Disease/therapy , Neural Pathways/physiology , Adult , Basal Ganglia/physiology , Cerebral Cortex/physiology , Cerebrovascular Circulation/physiology , Female , Humans , Huntington Disease/diagnostic imaging , Image Processing, Computer-Assisted , Male , Middle Aged , Nerve Net/physiology , Neurons/physiology , Oxygen Radioisotopes , Positron-Emission Tomography , Thalamus/physiologyABSTRACT
[(18)F]Altanserin is routinely used in several centers to study the serotonergic type-2 receptors (5HT(2)) with positron emission tomography (PET). An automatic production system allowing the preparation of multimillicurie amounts [>1.5 GBq (40 mCi) EOS, mean radiochemical yield 20 +/- 6% EOB, specific activity >1 Ci/µmol, n = 50] of this radiopharmaceutical within a synthesis time of 90 minutes (quality controls included) is described in this paper. The apparatus includes the recovery of the activity from the target, the preparation of the dried [(18)F]KF/kryptofix 2.2.2 complex, the labeling reaction using a microwave cavity, the Sep Pak and HPLC purification. A sterile, pyrogen-free and single use unit was also developed for the formulation of the injectable solution. This last part could be used for the formulation of many other radiopharmaceuticals.
