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1.
J Anim Physiol Anim Nutr (Berl) ; 87(1-2): 52-65, 2003 Feb.
Article in English | MEDLINE | ID: mdl-14511149

ABSTRACT

Five balance experiments on growing pigs were carried out to study the marginal efficiency of utilization of isoleucine, leucine, valine, histidine and phenylalanine + tyrosine and to estimate their requirements for maintenance. Purified diets based on casein and crystalline amino acids as the sole source of N contained graded levels of each amino acid, corresponding to protein accretion rates of 0, 33, 66, 99 and 132 g/day, respectively. All other essential amino acids were given in a 30% excess. N retention increased linearly (p < 0.01) as the dietary concentration of the limiting amino acid increased. Based on linear regression equations relating amino acid deposition in body protein to amino acid intake, marginal efficiencies of ileal digestible amino acid utilization were calculated to be isoleucine - 0.81, leucine - 0.81, valine - 0.82, histidine - 1.17 and phenylalanine + tyrosine - 0.67. Extrapolating the regression equations to zero N retention, the daily requirements of amino acids for N equilibrium were estimated to be (mg/kg0.75) isoleucine - 18, leucine - 33, valine - 23, histidine - 14 and phenylalanine + tyrosine - 43.


Subject(s)
Amino Acids, Essential/metabolism , Nitrogen/metabolism , Nutritional Requirements , Swine/growth & development , Amino Acids, Essential/administration & dosage , Animal Feed , Animal Nutritional Physiological Phenomena , Animals , Female , Ileum/metabolism , Random Allocation , Regression Analysis , Swine/metabolism , Tyrosine/administration & dosage , Tyrosine/metabolism
2.
J Anim Physiol Anim Nutr (Berl) ; 86(5-6): 153-65, 2002 Jun.
Article in English | MEDLINE | ID: mdl-15379917

ABSTRACT

A series of N balance experiments using growing pigs was conducted to study the efficiency of utilization of lysine, threonine, sulphur amino acids and tryptophan and to estimate their maintenance requirements. Purified diets based on casein and crystalline amino acids as the sole source of N contained graded levels of each amino acid, corresponding to expected protein accretion rate of 0, 33, 66, 99 and 132 g/day, respectively. N retention increased linearly (p < 0.01) as the dietary concentration of the limiting amino acid increased. Based on linear regression equations relating amino acid deposition in body protein to amino acid intake, marginal efficiencies of ileal digestible amino acid utilization were calculated to be lysine 0.91, threonine 0.83, sulphur amino acids 0.85 and tryptophan 0.66. Extrapolating the regression equations to zero N retention, the daily requirements of amino acids for N equilibrium were estimated to be (mg/kg(0.75)) lysine 39, threonine 49, sulphur amino acids 46 and tryptophan 16.


Subject(s)
Amino Acids/administration & dosage , Amino Acids/metabolism , Nitrogen/metabolism , Protein Biosynthesis , Swine/growth & development , Swine/metabolism , Amino Acids, Sulfur/administration & dosage , Amino Acids, Sulfur/metabolism , Animal Feed , Animal Nutritional Physiological Phenomena , Animals , Female , Lysine/administration & dosage , Lysine/metabolism , Nutritional Requirements , Random Allocation , Regression Analysis , Threonine/administration & dosage , Threonine/metabolism , Tryptophan/administration & dosage , Tryptophan/metabolism
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