ABSTRACT
Lipase has been purified from pig adipose tissue and antibodies have been produced in rabbit. By indirect immunofluorescence and immunoenzyme techniques lipase-like immunoreactivity was demonstrated in the intima of pig aorta, in the endothelial cells of the myocardium and in plasma membranes of adipocytes and skeletal muscle cells. Lipase activity in extracts of some of these tissues was inhibited by the addition of anti-lipase antibodies. At least part of the immunoreactivity in the examined tissues is due to active lipases.
Subject(s)
Adipose Tissue/enzymology , Aorta/enzymology , Lipase/metabolism , Myocardium/enzymology , Adipose Tissue/ultrastructure , Animals , Aorta/ultrastructure , Cross Reactions , Immunodiffusion , Immunoenzyme Techniques , Myocardium/ultrastructure , Swine , TriglyceridesABSTRACT
An enzymatic method for the histochemical localization of cholesterol is presented. It makes possible the localization of free cholesterol, cholesterol esters, or both and is compatible with routine histological staining procedures. The method is based on the production of H2O2 from free cholesterol by cholesterol oxidase. Sites of peroxide production are visualized by a brown reaction product formed in a peroxidase-catalysed reaction between diaminobenzidine and H2O2. Cholesterol esters can be demonstrated as cholesterol after hydrolysis by cholesterol ester hydrolase. Some examples of the application of the method are given.
