ABSTRACT
The clinical homogeneity of myeloproliferative syndromes associated with a 5 q- marker can be contested, but nevertheless finds support in the 2 case reports we have cited. One of these observations of refractory anemia with partial myeloblastosis and 5 q- marker has shown a transformation into an acute myelomonocytic leukemia with clonal chromosomal evolution.
Subject(s)
Anemia, Aplastic/genetics , Chromosome Aberrations , Chromosomes, Human, 4-5 , Aged , Cell Transformation, Neoplastic , Female , Humans , Leukemia, Monocytic, Acute/genetics , Leukemia, Myeloid, Acute/geneticsABSTRACT
Eleven samples from 7 primary and 4 metastatic male breast cancers were analysed for cytoplasmic oestrogen receptors. Two samples of metastatic cancers were obtained from a same patient 6 months apart. Receptors were assayed by measuring the binding affinity of cytosol fractions for 3H-oestradiol- 17beta. Saturable binding sites were obtained in 6 primary and 1 metastatic cancers. The dissociation constant of the binding reactions were within the range reported for the specific oestrogen receptors from female breast cancers. When present, receptor concentrations varied from 59 to 532 fmoles/mg tissue protein. Competition studies of the binding of 3H-oestradiol- 17beta by excess amounts of unlabelled steroid hormones or antihormones revealed that the receptors were specific for oestrogens and antioestrogens. By all these criteria, receptors from male mammary cancer were identical to those from female breast cancer. Chromosome analysis was performed in 6 patients. All had normal 46 XY male pattern indicating that no conspicuous genetic abnormalities such as those of the Klinefelter syndrome seemed responsible for the presence of the receptors.
Subject(s)
Breast Neoplasms/analysis , Receptors, Estrogen/analysis , Aged , Binding Sites , Binding, Competitive , Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Cytosol/metabolism , Estradiol/metabolism , Female , Hormones/metabolism , Humans , In Vitro Techniques , Karyotyping , Male , Middle Aged , Radioligand Assay , Receptors, Estrogen/metabolismABSTRACT
A further instance of the 5q-chromosome associated with a distinct type of aregenerative anemia strengthened the hypothesis of specificity and consistency of this karyotypic abnormality in a new hematologic entity. The Giemsa banding technique revealed that the deletion of the long arms of chromosome number 5 was interstitial: del (5) (pter leads to q12 : : q31 leads to qter).
