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1.
Proc Natl Acad Sci U S A ; 111(9): 3579-84, 2014 Mar 04.
Article in English | MEDLINE | ID: mdl-24522108

ABSTRACT

The thermosensor DesK is a multipass transmembrane histidine-kinase that allows the bacterium Bacillus subtilis to adjust the levels of unsaturated fatty acids required to optimize membrane lipid fluidity. The cytoplasmic catalytic domain of DesK behaves like a kinase at low temperature and like a phosphatase at high temperature. Temperature sensing involves a built-in instability caused by a group of hydrophilic residues located near the N terminus of the first transmembrane (TM) segment. These residues are buried in the lipid phase at low temperature and partially "buoy" to the aqueous phase at higher temperature with the thinning of the membrane, promoting the required conformational change. Nevertheless, the core question remains poorly understood: How is the information sensed by the transmembrane region converted into a rearrangement in the cytoplasmic catalytic domain to control DesK activity? Here, we identify a "linker region" (KSRKERERLEEK) that connects the TM sensor domain with the cytoplasmic catalytic domain involved in signal transmission. The linker adopts two conformational states in response to temperature-dependent membrane thickness changes: (i) random coiled and bound to the phospholipid head groups at the water-membrane interface, promoting the phosphatase state or (ii) unbound and forming a continuous helix spanning a region from the membrane to the cytoplasm, promoting the kinase state. Our results uphold the view that the linker is endowed with a helix/random coil conformational duality that enables it to behave like a transmission switch, with helix disruption decreasing the kinase/phosphatase activity ratio, as required to modulate the DesK output response.


Subject(s)
Bacillus subtilis/metabolism , Membrane Fluidity/physiology , Membrane Proteins/metabolism , Protein Conformation , Protein Kinases/metabolism , Signal Transduction/physiology , Thermosensing/physiology , Amino Acid Sequence , Bacillus subtilis/genetics , Chromatography, High Pressure Liquid , Electrophoresis, Polyacrylamide Gel , Escherichia coli , Histidine Kinase , Mass Spectrometry , Molecular Sequence Data , Phosphorylation , Signal Transduction/genetics , Spectroscopy, Fourier Transform Infrared , Temperature , Thermosensing/genetics
2.
Nanomedicine ; 10(4): 775-82, 2014 May.
Article in English | MEDLINE | ID: mdl-24361386

ABSTRACT

We provide evidence that cationic lipids, usually considered as a safe alternative to viral vectors as nanocarriers for gene therapy or drug intracellular delivery, do not behave as inert material but do activate cellular signalling pathways implicated in inflammatory reactions. We show here that the cationic lipid RPR206252 induces NF-κB activation, and the production of TNF-α, IL-1ß, IL-6 and IFN-γ by human or mouse macrophage cell lines. Further, we demonstrate that the activation of inflammatory cascades by RPR206252 is dependent on Toll-like receptor 2 (TLR2), the natural sensor of bacterial lipopeptides and NOD-like receptor protein 3 (NLRP3), the major inflammasome component. Our results suggest that cationic lipid nanocarriers because of their ability to stimulate the innate system can be used as a new class of synthetic and safe adjuvant for vaccination. FROM THE CLINICAL EDITOR: Cationic lipid nanocarriers are typically considered neutral tools for gene delivery. However, as demonstrated in this study, they possess a clear ability to stimulate the innate immune system, and actually can be used as a new class of synthetic and safe adjuvant for vaccination.


Subject(s)
Carrier Proteins/immunology , Drug Carriers/pharmacology , Lipids/pharmacology , Nanoparticles , Toll-Like Receptor 2/immunology , Animals , Cytokines/immunology , Drug Carriers/chemistry , HEK293 Cells , Humans , Immunity, Innate/drug effects , Lipids/chemistry , Mice , NLR Family, Pyrin Domain-Containing 3 Protein
3.
Adv Drug Deliv Rev ; 64(15): 1749-58, 2012 Dec.
Article in English | MEDLINE | ID: mdl-22634161

ABSTRACT

Cationic liposomes are commonly used as a transfection reagent for DNA, RNA or proteins and as a co-adjuvant of antigens for vaccination trials. A high density of positive charges close to cell surface is likely to be recognized as a signal of danger by cells or contribute to trigger cascades that are classically activated by endogenous cationic compounds. The present review provides evidence that cationic liposomes activate several cellular pathways like pro-apoptotic and pro-inflammatory cascades. An improved knowledge of the relationship between the cationic lipid properties (nature of the lipid hydrophilic moieties, hydrocarbon tail, mode of organization) and the activation of these pathways opens the way to the use and design of cationic tailored for a specific application (e.g. for gene transport or as adjuvants).


Subject(s)
Adjuvants, Immunologic/administration & dosage , Lipids/chemistry , Vaccines/immunology , Animals , Antigens/immunology , Apoptosis/drug effects , Cations , Drug Design , Humans , Inflammation/chemically induced , Inflammation/pathology , Liposomes , Signal Transduction/drug effects , Transfection
4.
Langmuir ; 28(10): 4640-7, 2012 Mar 13.
Article in English | MEDLINE | ID: mdl-22332736

ABSTRACT

In this work, we investigated the properties of a fusogenic cationic lipid, diC14-amidine, and show that this lipid possesses per se the capacity to adopt either an interdigitated structure (below and around its transition temperature) or a lamellar structure (above the transition temperature). To provide experimental evidence of this lipid bilayer organization, phospholipids spin-labeled at different positions of the hydrocarbon chain were incorporated into the membrane and their electron spin resonance (ESR) spectra were recorded at different temperatures. For comparison, similar experiments were performed with dimyristoyl phosphatidylcholine, a zwitterionic lipid (DMPC) which adopts a bilayer organization over a broad temperature range. Lipid mixing between diC14-amidine and asolectin liposomes was more efficient below (10-15 °C) than above the transition temperature (above 25 °C). This temperature-dependent "fusogenic" activity of diC14-amidine liposomes is opposite to what has been observed so far for peptides or virus-induced fusion. Altogether, our data suggest that interdigitation is a highly fusogenic state and that interdigitation-mediated fusion occurs via an unusual temperature-dependent mechanism that remains to be deciphered.


Subject(s)
Lipid Bilayers/chemistry , Amidines/chemistry , Cations , Dimyristoylphosphatidylcholine/chemistry , Electron Spin Resonance Spectroscopy , Fluorescence Polarization , Liposomes/chemistry , Membrane Fusion/physiology , Models, Biological , Models, Molecular , Phase Transition , Phosphatidylcholines/chemistry , Temperature
5.
Vaccine ; 30(2): 414-24, 2012 Jan 05.
Article in English | MEDLINE | ID: mdl-22075093

ABSTRACT

Adjuvant efficiency is critical for inducing a protective and long-lasting immune response against weak immunogenic antigens. Discovered more than 70 years ago, aluminum salts remain the most widely used adjuvant in human vaccine. Prone to induce a strong humoral response, alum fails to drive a cell-mediated immunity, which is essential to fight against intracellular pathogens. Adjuvant systems that contain more than one component may represent an excellent alternative for completing the lack of T cell immunity associated with the injection of alum-based vaccine. In this work, we demonstrated that the adjuvant effects of alum strongly benefited from combining with a cationic lipid, the diC14 amidine. Indeed, we measured a significant improvement of alum-driven IL-1ß release when human macrophages were co-cultured with a mixed suspension of alum and the diC14 amidine. Morphological analysis suggested that diC14 amidine improved the alum uptake by phagocytes. Furthermore, the addition of diC14 amidine to alum efficiently enhanced antigen processing and cross-presentation by antigen presenting cells. The biological relevance of these in vitro data was assessed by measuring the in vivo development of a cytotoxic activity and the enhanced synthesis of antigen-specific immunoglobulins after immunization with alum combined to diC14 amidine. Mechanistically, we demonstrated that diC14 amidine supported the alum adjuvanticity independently of the TLR-4 and caspase-1 agonist activities of the cationic lipid. Based on our findings, we conclude that diC14 amidine works synergistically with alum to achieve higher immune protection after vaccination.


Subject(s)
Adjuvants, Immunologic/administration & dosage , Alum Compounds/administration & dosage , Amidines/administration & dosage , Caspase 1/immunology , Toll-Like Receptor 4/immunology , Animals , Cells, Cultured , Female , Immunity, Cellular , Immunity, Humoral , Interleukin-1beta/metabolism , Macrophages/immunology , Male , Mice , Mice, Inbred C57BL , Models, Animal , Ovalbumin/immunology , Vaccines, Subunit/administration & dosage , Vaccines, Subunit/immunology
6.
Biochim Biophys Acta ; 1798(3): 569-78, 2010 Mar.
Article in English | MEDLINE | ID: mdl-19913495

ABSTRACT

Peritoneal macrophages were isolated from wild type (WT) mice and from mice invalidated for the P2X(7) receptor (KO) which had been pretreated with thioglycolate. In cells from WT mice, 1 mM ATP increased the intracellular concentration of calcium ([Ca(2+)](i)), the uptake of ethidium bromide, the production of reactive oxygen species (ROS), the secretion of IL-1beta, the release of oleic acid and of lactate dehydrogenase; it decreased the intracellular concentration of potassium ([K(+)](i)). In KO mice, ATP transiently increased the [Ca(2+)](i) confirming that the P2X(7) receptor is a major receptor of peritoneal macrophages. WKYMVm, an agonist of receptors for formylated peptides (FPR) also increased the [Ca(2+)](i) in murine macrophages. The slight increase of the [Ca(2+)](i) was strongly potentiated by ivermectin confirming the expression of functional P2X(4) receptors by murine peritoneal macrophages. CRAMP, the unique antimicrobial peptide derived from cathelin in mouse inhibited all the responses coupled to P2X(7) receptors in macrophages from WT mice. Agonists for FPR had no effect on the increase of the [Ca(2+)](i) in response to ATP. CRAMP had no effect on the increase of the [Ca(2+)](i) evoked by a combination of ATP and ivermectin in macrophages from P2X(7)-KO mice. In summary CRAMP inhibits the responses secondary to the activation of the murine P2X(7) receptors expressed by peritoneal macrophages. This inhibition is not mediated by FPR receptors and is specific since CRAMP has no effect on the response coupled to P2X(4) receptors. It can thus be concluded that the interaction between P2X(7) receptors and cathelin-derived antimicrobial peptides is species-specific, in some cases (man) positive in others (mouse) negative.


Subject(s)
Adenosine Triphosphate/pharmacology , Cathelicidins/pharmacology , Extracellular Space/drug effects , Extracellular Space/metabolism , Macrophages, Peritoneal/drug effects , Macrophages, Peritoneal/metabolism , Animals , Antimicrobial Cationic Peptides , Calcium/metabolism , Cathelicidins/chemistry , Ethidium/metabolism , Interleukin-1beta/metabolism , Ion Channel Gating/drug effects , Ion Channels/metabolism , Male , Mice , Mice, Knockout , Oleic Acid/metabolism , Potassium/metabolism , Protein Structure, Secondary , Reactive Oxygen Species/metabolism , Receptors, Formyl Peptide/agonists , Spectrophotometry, Infrared
7.
Cell Mol Life Sci ; 67(3): 483-94, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19924382

ABSTRACT

Addition of co-lipids into cationic lipid formulations is considered as promoting cell delivery of DNA by enhancing fusion processes with cell membranes. Here, by combining FRET and confocal microscopy, we demonstrate that some cationic lipids do not require a co-lipid to fuse efficiently with cells. These cationic lipids are able to self-organize into bilayers that are stable enough to form liposomes, while presenting some destabilizing properties reminiscent of the conically shaped fusogenic co-lipid, DOPE. We therefore analyzed the resident lipid structures in cationic bilayers by molecular dynamics simulations, clustering the individual lipid structures into populations of similarly shaped molecules, as opposed to the classical approach of using the static packing parameter to define the lipid shapes. Comparison of fusogenic properties with these lipid populations suggests that the ratio of cylindrical versus conical lipid populations correlates with the ability to fuse with cell membranes.


Subject(s)
Cations/chemistry , Lipids/chemistry , Animals , Cell Line, Tumor , Cell Membrane/chemistry , Cell Membrane/metabolism , Fatty Acids, Monounsaturated/chemistry , Fluorescence Resonance Energy Transfer , Gene Transfer Techniques , Liposomes/chemistry , Liposomes/metabolism , Mice , Microscopy, Confocal , Molecular Dynamics Simulation , Phosphatidylethanolamines/chemistry , Quaternary Ammonium Compounds/chemistry
8.
Biochim Biophys Acta ; 1790(6): 425-30, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19269309

ABSTRACT

Cationic lipids have been extensively used as carriers of biologically active molecules (nucleic acids, peptides and proteins) into cells. Recent data provided evidence that cationic lipids are not just inert transporters but do activate specific cellular cascades. This review illustrates these activating properties with a few examples. Cell activation raises the question of which receptors are involved. Some cationic lipids seem to satisfy specific structural requirements of Toll-like receptors (TLR4) as they activate TLR4-dependent pathways. However, cationic lipids display a large structural diversity and it is likely that they are also recognized by receptors with a broader specificity. Alternatives are proposed and discussed to explain this broad specificity.


Subject(s)
Cations/metabolism , Lipid Metabolism , Lipids/chemistry , Signal Transduction/physiology , Cations/chemistry , Integrins/metabolism , Lymphocyte Antigen 96/chemistry , Lymphocyte Antigen 96/metabolism , Mitogen-Activated Protein Kinases/metabolism , Toll-Like Receptor 4/metabolism
9.
Langmuir ; 25(9): 5230-8, 2009 May 05.
Article in English | MEDLINE | ID: mdl-19231818

ABSTRACT

DiC(14)-amidine (amidine) is a nonphysiological, cationic lipid that forms stable liposomes under physiological pH and temperature. Cationic lipids have been proposed as delivery vector for DNA, proteins, and drugs. Furthermore, amidine carries at present a particular interest due to its immunomodulatory properties. (1-3) Molecular dynamics simulations reveal a remarkable fluidity in the hydrophobic bilayer core, with a tendency for strong surface curvature, in agreement with the relatively small size of experimentally formed liposomes. The amidine bilayer shows an interdigitated, nonlamellar bilayer phase, with a bilayer thickness of only 2.7 nm and an average area per lipid of 0.83 nm(2). A cluster analysis of the individual lipid structures shows a thermally accessible population of V-shaped lipids, indicative of fusion capabilities with the plasma membrane. Fusion experiments confirm this hypothesis. The results are compared to the zwitterionic DMPC (dimyristoylphosphocholine), which also carries two saturated C(14) tails.


Subject(s)
Amidines/chemistry , Dimyristoylphosphatidylcholine/chemistry , Lipid Bilayers/chemistry , Myristic Acid/chemistry , Cations/chemistry , Computer Simulation , Models, Molecular , Molecular Structure
10.
J Allergy Clin Immunol ; 123(5): 1150-6, 2009 May.
Article in English | MEDLINE | ID: mdl-19152967

ABSTRACT

BACKGROUND: Recombinant hypoallergens that display reduced allergenicity but retain T-cell reactivity represent promising candidates to improve the safety and efficacy of allergen-specific vaccines or immunotherapy. OBJECTIVE: The current study reports the immunologic characterization of a hypoallergenic variant of the major mite allergen Der p 1. METHODS: The recombinant proform of Der p 1 (ProDer p 1) was expressed in Escherichia coli (ProDer p 1 coli), purified and characterized at the level of its secondary structure, and IgE and T-cell reactivities. Moreover, the prophylactic potential of ProDer p 1 coli vaccinations was evaluated in a murine Der p 1 sensitization model. RESULTS: After purification and refolding, ProDer p 1 coli remained aggregated with a higher beta-sheet content and altered Der p 1 conformational epitopes compared with the correctly folded monomeric ProDer p 1 produced in Chinese hamster ovary cells. Both ProDer p 1 forms were able to retain the Der p 1-specific T-cell reactivity but direct ELISA, competitive inhibition, and rat basophil leukemia assays clearly showed that ProDer p 1 coli displays a very weak IgE reactivity. Mice vaccinations with aggregated ProDer p 1 adjuvanted with alum induced a T(H)1-biased immune response that prevented the subsequent allergic response after Der p 1 sensitization and airway challenge with aerosolized mite extracts. Furthermore, ProDer p 1 coli treatment inhibited the development of airway eosinophilia and airway hyperresponsiveness to inhaled methacholine. CONCLUSION: Aggregated forms of Der p 1 could represent hypoallergens suitable for the prevention of mite allergy.


Subject(s)
Allergens/immunology , Hypersensitivity/prevention & control , Pyroglyphidae/immunology , Vaccines/immunology , Animals , Antigens, Dermatophagoides/genetics , Antigens, Dermatophagoides/isolation & purification , Antigens, Dermatophagoides/pharmacology , Arthropod Proteins , Basophils/drug effects , Basophils/immunology , Basophils/metabolism , Bronchial Hyperreactivity/immunology , Bronchial Hyperreactivity/prevention & control , Bronchoconstrictor Agents/pharmacology , Cell Line, Tumor , Cloning, Molecular , Cysteine Endopeptidases , Disease Models, Animal , Eosinophilia/immunology , Eosinophilia/prevention & control , Female , Humans , Hypersensitivity/immunology , Immunoglobulin E/blood , Interferon-gamma/biosynthesis , Interferon-gamma/immunology , Interleukin-5/biosynthesis , Interleukin-5/immunology , Methacholine Chloride/pharmacology , Mice , Mice, Inbred BALB C , Rats , Recombinant Proteins/genetics , Recombinant Proteins/immunology , T-Lymphocytes/drug effects , T-Lymphocytes/immunology , T-Lymphocytes/metabolism
11.
J Biol Chem ; 283(45): 30606-17, 2008 Nov 07.
Article in English | MEDLINE | ID: mdl-18725410

ABSTRACT

The trypsin-like protease Der p 3, a major allergen of the house dust mite Dermatophagoides pteronyssinus, is synthesized as a zymogen, termed proDer p 3. No recombinant source of Der p 3 has been described yet, and the zymogen maturation mechanism remains to be elucidated. The Der p 3 zymogen was produced in Pichia pastoris. We demonstrated that the recombinant zymogen is glycosylated at the level of its propeptide. We showed that the activation mechanism of proDer p 3 is intermolecular and is mediated by the house dust mite cysteine protease Der p 1. The primary structure of the proDer p 3 propeptide is associated with a unique zymogen activation mechanism, which is different from those described for the trypsin-like family and relies on the house dust mite papain-like protease Der p 1. This is the first report of a recombinant source of Der p 3, with the same enzymatic activity as the natural enzyme and trypsin. Glycosylation of the propeptide was found to decrease the rate of maturation. Finally, we showed that recombinant Der p 3 is inhibited by the free modified prosequence T(P1)R.


Subject(s)
Antigens, Dermatophagoides/chemistry , Cysteine Endopeptidases/chemistry , Dermatophagoides pteronyssinus/enzymology , Enzyme Precursors/chemistry , Amino Acid Sequence , Animals , Antigens, Dermatophagoides/genetics , Arthropod Proteins , Cysteine Endopeptidases/genetics , Dermatophagoides pteronyssinus/genetics , Enzyme Activation , Enzyme Precursors/genetics , Glycosylation , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Serine Endopeptidases
12.
Eur J Pharm Biopharm ; 69(3): 817-23, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18407478

ABSTRACT

Cationic lipids are widely used as vectors to deliver DNA into mammalian cells in vitro and in vivo. However, cationic lipid/DNA lipoplexes induce an inflammatory response, characterized by pro-inflammatory cytokine secretion, which severely limits their use. The main goal of this work is to identify the organs and the cell type involved in TNF-alpha secretion after lipoplex injection. We determined the kinetics of distribution of the cationic lipid/DNA complex in blood, lung, liver and spleen and quantified the TNF-alpha amount in organ homogenates and in the serum at different points of times. Increase in TNF-alpha production was only observed in the spleen and no significant increase of TNF-alpha production could be observed in the other organs. Fractionation of spleen cells revealed that macrophages were mainly responsible for TNF-alpha secretion. This observation was verified in vivo by using macrophage-removing agents. In conclusion, we show here that the TNF-alpha secreted in the serum after intravenous injection of lipoplexes comes mainly from the splenic macrophages.


Subject(s)
DNA/toxicity , Lipids/toxicity , Macrophages/metabolism , Spleen/metabolism , Tumor Necrosis Factor-alpha/biosynthesis , Animals , Cations/chemistry , Cell Adhesion , Cell Separation , Cytokines/analysis , Cytokines/biosynthesis , DNA/chemistry , DNA/pharmacokinetics , Female , Inflammation/chemically induced , Inflammation/pathology , Injections, Intravenous , Lipids/chemistry , Lipids/pharmacokinetics , Liposomes/chemistry , Macrophages/drug effects , Mice , Mice, Inbred BALB C , Plasmids/chemistry , Plasmids/genetics , Spleen/cytology , Spleen/drug effects , Tissue Distribution , Transfection
13.
Prog Lipid Res ; 47(5): 340-7, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18424270

ABSTRACT

Cationic lipids are positively charged amphiphilic molecules which, for most of them, form positively charged liposomes, sometimes in combination with a neutral helper lipid. Such liposomes are mainly used as efficient DNA, RNA or protein carriers for gene therapy or immunization trials. Over the past decade, significant progress has been made in the understanding of the cellular pathways and mechanisms involved in lipoplex-mediated gene transfection but the interaction of cationic lipids with cell components and the consequences of such an interaction on cell physiology remains poorly described. The data reported in the present review provide evidence that cationic lipids are not just carriers for molecular delivery into cells but do modify cellular pathways and stimulate immune or anti-inflammatory responses. Considering the wide number of cationic lipids currently available and the variety of cellular components that could be involved, it is likely that only a few cationic lipid-dependent functions have been identified so far.


Subject(s)
Lipids/physiology , Liposomes/metabolism , Animals , Biological Transport/physiology , Cations , Cell Membrane/metabolism , Immunity, Cellular/physiology , Lipids/chemistry , Lipids/genetics , Mice , Signal Transduction/physiology , Transfection
14.
Eur J Immunol ; 38(5): 1351-7, 2008 May.
Article in English | MEDLINE | ID: mdl-18389479

ABSTRACT

DiC14-amidine cationic liposomes were recently shown to promote Th1 responses when mixed with allergen. To further define the mode of action of diC14-amidine as potential vaccine adjuvant, we characterized its effects on mouse and human myeloid dendritic cells (DC). First, we observed that, as compared with two other cationic liposomes, only diC14-amidine liposomes induced the production of IL-12p40 and TNF-alpha by mouse bone marrow-derived DC. DiC14-amidine liposomes also activated human DC, as shown by synthesis of IL-12p40 and TNF-alpha, accumulation of IL-6, IFN-beta and CXCL10 mRNA, and up-regulation of membrane expression of CD80 and CD86. DC stimulation by diC14-amidine liposomes was associated with activation of NF-kappaB, ERK1/2, JNK and p38 MAP kinases. Finally, we demonstrated in mouse and human cells that diC14-amidine liposomes use Toll-like receptor 4 to elicit both MyD88-dependent and Toll/IL-1R-containing adaptor inducing interferon IFN-beta (TRIF)-dependent responses.


Subject(s)
Adjuvants, Immunologic/pharmacology , Amidines/pharmacology , Dendritic Cells/drug effects , Toll-Like Receptor 4/agonists , Adaptor Proteins, Vesicular Transport/genetics , Adjuvants, Immunologic/administration & dosage , Amidines/administration & dosage , Animals , Cell Line , Cytokines/metabolism , Dendritic Cells/metabolism , Female , Humans , Interleukin-12 Subunit p40/metabolism , Lymphocyte Antigen 96/genetics , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Mice, Mutant Strains , Mitogen-Activated Protein Kinases/metabolism , Myeloid Cells/cytology , Myeloid Differentiation Factor 88/genetics , NF-kappa B/metabolism , Phosphorylation/drug effects , Toll-Like Receptor 4/genetics , Toll-Like Receptors/genetics , Transfection , Tumor Necrosis Factor-alpha/metabolism
15.
Vet Res ; 38(6): 819-34, 2007.
Article in English | MEDLINE | ID: mdl-17727807

ABSTRACT

We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes.


Subject(s)
Bovine Virus Diarrhea-Mucosal Disease/immunology , Bovine Virus Diarrhea-Mucosal Disease/prevention & control , Diarrhea Viruses, Bovine Viral/immunology , Vaccines, DNA/immunology , Viral Envelope Proteins/chemistry , Viral Envelope Proteins/immunology , Viral Vaccines/immunology , Administration, Intranasal , Animals , Antibodies, Viral/blood , Base Sequence , Cattle , Cloning, Molecular , Injections, Intradermal , Injections, Intramuscular , Male , Vaccines, DNA/administration & dosage , Vaccines, DNA/genetics , Viral Envelope Proteins/genetics , Viral Vaccines/administration & dosage , Viral Vaccines/chemistry , Viral Vaccines/genetics
16.
Mol Membr Biol ; 24(3): 225-32, 2007.
Article in English | MEDLINE | ID: mdl-17520479

ABSTRACT

Cationic lipids are efficient tools to introduce nucleic acids and proteins into cells. Elucidation of the mechanism and cellular pathways associated with such transport has been relatively tedious, even though significant progress has been made in the characterization of the intracellular trafficking of lipid/DNA complexes. Surprisingly little is known about the effects of these delivery vectors on cell functioning. In this report, we show that both cationic lipids and cationic lipid/DNA complexes mobilize the intracellular calcium. Removal of extracellular calcium did not significantly abolish this effect and preincubating cells with thapsigargin led to a decrease in [Ca2+]i, indicating that calcium was released mainly from internal calcium stores sensitive to thapsigargin. Pretreatment of the cells with the phospholipase C inhibitor U73122, blocked the [Ca2+]i rise, suggesting an inositol dependent mechanism.


Subject(s)
Calcium Signaling/drug effects , Calcium/metabolism , Cations/metabolism , Cytosol/metabolism , Lipid Metabolism , Transfection , Biological Transport , Cations/chemistry , Enzyme Inhibitors/pharmacology , Estrenes/chemistry , Estrenes/pharmacology , Genetic Vectors/metabolism , Humans , Inositol Phosphates/metabolism , K562 Cells , Lipids/chemistry , Liposomes , Models, Biological , Pyrrolidinones/chemistry , Pyrrolidinones/pharmacology , Thapsigargin/chemistry , Thapsigargin/pharmacology , Type C Phospholipases/antagonists & inhibitors , Type C Phospholipases/metabolism
17.
Mol Membr Biol ; 23(3): 227-34, 2006.
Article in English | MEDLINE | ID: mdl-16785206

ABSTRACT

It has been shown that a preinjection of diC14-amidine cationic liposomes decreased TNF-alpha secretion induced by lipoplexes intravenous injection. We showed here that free cationic liposomes inhibit CpG sequences- or lipopolysaccharides-induced TNF-alpha secretion by macrophages. Surprisingly, this effect was strictly dependent on serum. Free cationic liposomes alone did not reveal any anti-inflammatory activity. Low-density lipoproteins and triglyceride-rich lipoproteins were identified as the serum components that confer to the liposomes an anti-inflammatory activity. Lipid fractions of these lipoproteins were able to reproduce the effect of the total lipoproteins and could inhibit, in association with diC14-amidine liposomes, the CpG-induced TNF-alpha secretion. Serum components confer to cationic liposomes new properties that can be used to modulate the inflammatory response directed against CpG sequences and lipopolysaccharides.


Subject(s)
Amidines/pharmacology , CpG Islands , Lipopolysaccharides/metabolism , Lipoproteins/metabolism , Tumor Necrosis Factor-alpha/metabolism , Amidines/metabolism , Animals , Anti-Inflammatory Agents/metabolism , Cells, Cultured , Humans , Lipoproteins, LDL/metabolism , Liposomes/pharmacology , Macrophages/drug effects , Macrophages/metabolism , Mice , Oligonucleotides/metabolism , Triglycerides/metabolism
18.
Mol Pharmacol ; 69(6): 2037-46, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16514052

ABSTRACT

The interaction of mice submandibular gland cells with LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES), a cationic peptide with immunomodulatory properties, was investigated. LL-37 at a concentration that did not affect the integrity of the cells increased the uptake of calcium and activated a calcium-insensitive phospholipase A(2) (PLA(2)). The small release of ATP induced by LL-37 could not account for this stimulation because apyrase did not significantly block the response to LL-37. The divalent cation magnesium inhibited the response to LL-37, but this inhibition was probably nonspecific because it also inhibited the in vitro bacteriostatic effect of the peptide. The increase of calcium uptake by LL-37 was not affected by 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN-62), a rather specific inhibitor of P2X(7) receptors in mice. LL-37 also increased [Ca(2+)](i) in cells from mice invalidated for these receptors. LL-37 had no effect on the response to carbachol. It inhibited the increase of [Ca(2+)](i) and the activation of phospholipase D by ATP. It potentiated the activation of the PLA(2) by the nucleotide. Finally, LL-37 increased the fluidity of the plasma membrane of submandibular gland cells. In conclusion, our results suggest that LL-37 is an autocrine regulator of submandibular gland cells. It does not stimulate mouse P2X(7) receptors but modulates their responses.


Subject(s)
Antimicrobial Cationic Peptides/pharmacology , Purinergic P2 Receptor Agonists , Submandibular Gland/drug effects , 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives , 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology , Adenosine Triphosphate/metabolism , Adenosine Triphosphate/pharmacology , Animals , Antimicrobial Cationic Peptides/antagonists & inhibitors , Antimicrobial Cationic Peptides/metabolism , Calcium/metabolism , Cations, Divalent/pharmacology , Cell Membrane/drug effects , Enzyme Inhibitors/pharmacology , Group IV Phospholipases A2 , Magnesium/pharmacology , Male , Membrane Fluidity/drug effects , Mice , Mice, Inbred C57BL , Muscarinic Agonists/metabolism , Muscarinic Agonists/pharmacology , Phospholipases A/drug effects , Receptors, Purinergic P2X7 , Salivary Glands/drug effects , Cathelicidins
19.
Mol Ther ; 11(6): 960-8, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15922967

ABSTRACT

The present study evaluated the prophylactic potential of ProDer p 1, the recombinant precursor form of the major mite allergen Der p 1, combined with the cationic lipid diC14-amidine in a murine model of house dust mite allergy. Naive mice vaccinated with the amidine/allergen complex developed a Th1-biased immune response characterized by the absence of specific IgE, the production of specific IgG2a, and the presence of IFN-gamma in splenocyte cultures. In contrast, ProDer p 1 adjuvanted with alum induced typical strictly Th2-biased allergic responses with strong IgG1 and IgE titers and IL-5 secretion. Removal of negatively charged sialic acids in ProDer p 1 or increasing the ionic strength reduced the binding of ProDer p 1 to the cationic liposomes and resulted in a decrease of the allergen immunogenicity, suggesting that complexation is required for triggering an optimal immune response. Finally, prophylactic vaccination with ProDer p 1-diC14-amidine reduced drastically the production of specific IgE and airway eosinophilia following subsequent immunization with Der p 1-alum and challenge with aerosolized house dust mite extracts. In conclusion, recombinant ProDer p 1 complexed with diC14-amidine could represent an efficient prophylactic vaccine against house dust mite allergy.


Subject(s)
Allergens/immunology , Antigens, Dermatophagoides/immunology , Dermatophagoides pteronyssinus/immunology , Protein Precursors/immunology , Respiratory Hypersensitivity/prevention & control , Vaccines , Allergens/chemistry , Amidines/chemistry , Amidines/immunology , Animals , Antigens, Dermatophagoides/chemistry , Arthropod Proteins , Cations/chemistry , Female , Immunoglobulin E/metabolism , Immunoglobulin G/metabolism , Interferon-gamma/metabolism , Lipids/chemistry , Mice , Mice, Inbred BALB C , Protein Precursors/chemistry , Recombinant Proteins/immunology , Th1 Cells/immunology
20.
J Biol Chem ; 280(13): 12255-61, 2005 Apr 01.
Article in English | MEDLINE | ID: mdl-15661732

ABSTRACT

It has been previously shown that transfection activity of cationic liposome/DNA lipoplexes delivered systemically is drastically inhibited by lipoproteins (Tandia, B. M., Vandenbranden, M., Wattiez, R., Lakhdar, Z., Ruysschaert, J. M., and Elouahabi, A. (2003) Mol Ther. 8, 264-273). In this work, we have compared the binding/uptake and transfection activities of DOTAP (N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride) and diC14-amidine (3-tetradecylamino-N-tert-butyl-N'-tetra-decylpropionamidine)-containing lipoplexes in the presence or absence of purified low density lipoproteins and high density lipoprotein. Binding/uptake of both lipoplexes by the mouse lung endothelial cell line was inhibited to a similar extent in the presence of lipoproteins. In contrast, transfection activity of diC14-amidine-containing lipoplexes was almost completely inhibited (approximately by 95%), whereas approximately 40% transfection activity of DOTAP-containing lipoplexes was preserved in the presence of lipoproteins. Interestingly, the ability of lipoproteins to inhibit the transfection efficiency of lipoplexes was well correlated with their ability to undergo lipid mixing with the cationic lipid bilayer as revealed by fluorescence resonance energy transfer assay. Incubation of lipoplexes with increased doses of lipoproteins resulted in enhanced lipid mixing and reduced transfection activity of the lipoplexes in mouse lung endothelial cells. The role of lipid mixing in transfection was further demonstrated using lipid-mixing inhibitor, lyso-phosphatidylcholine, or activator (dioleoylphosphatidylethanolamine). Incorporation of Lyso-PC into diC14-amidine-containing lipoplexes completely abolished their capacity to undergo lipid mixing with lipoproteins and allowed them to reach a high transfection efficiency in the presence of lipoproteins. On the other hand, the incorporation of dioleoylphosphatidylethanolamine into DOTAP/DNA lipoplex activated lipid mixing with the lipoproteins and was shown to be detrimental toward the transfection activity of these lipoplexes. Taken together, these results indicate that fusion of lipoplexes with lipoproteins is a limiting factor for in vivo transfection.


Subject(s)
Lipid Metabolism , Lipoproteins/blood , Lipoproteins/chemistry , Animals , Calorimetry, Differential Scanning , Cations , DNA/metabolism , Detergents/pharmacology , Dose-Response Relationship, Drug , Endothelial Cells/metabolism , Fatty Acids, Monounsaturated/chemistry , Fluorescence Resonance Energy Transfer , Gene Transfer Techniques , Lipid Bilayers/chemistry , Lipids/chemistry , Lipoproteins, HDL/metabolism , Liposomes/metabolism , Lung/cytology , Lysophosphatidylcholines/metabolism , Mice , Phosphatidylethanolamines/metabolism , Plasmids/metabolism , Protein Binding , Quaternary Ammonium Compounds/chemistry , Temperature , Time Factors , Transfection , Trypsin/pharmacology
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