Correlation of the extent of coronary occlusion with Apo B levels. Application of a new enzyme immunoassay technique for Apo B.

A new rapid and sensitive method for measurement of apolipoprotein B (Apo B) in plasma has been developed. This method, based on a Competitive Enzyme-Linked Immunoassay (CELIA), has been used to study the association between the extent of coronary artery occlusion as measured by coronary arteriography and the levels of plasma Apo B. The correlation between Apo B levels and some other plasma lipids was also determined. Significant relationship was found between the extent of coronary artery occlusion and Apo B, as well as between Apo B and plasma total cholesterol and plasma triglyceride levels.

Examination of a competitive enzyme-linked immunoassay (CELIA) technique and a laser nephelometric immunoassay technique for the measurement of apolipoprotein B.

A competitive enzyme-linked immunoassay (CELIA) technique for quantitative measurement of apolipoprotein B (Apo B) was developed. The method is a non-isotopic immunoassay that utilizes a soluble enzyme/antibody complex as a universal labeling reagent. The method was characterized according to precision, sensitivity, recovery and parallelism. The CELIA Apo B method was compared to a commercially available laser nephelometric immunoassay. We found that the nephelometric results were highly correlated with triglyceride levels and the nephelometric assay was susceptible to interference from lipemia or turbidity. The range of values obtained on 56 apparently healthy, fasting young adults was 0.35-1.25 g/l by the CELIA method and 0.40-1.00 g/l by the nephelometric immunoassay. The nephelometric method was more precise (coefficient of variation 5%) than the CELIA technique (CV 10%); however, the CELIA method seems to be less sensitive to interferences.