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Colloids Surf B Biointerfaces ; 101: 205-9, 2013 Jan 01.
Article in English | MEDLINE | ID: mdl-23010020

ABSTRACT

A new method for detection of viruses has been developed. The entire assay can be performed within 2h, and consists of a polyelectrolyte-multilayer-modified cellulosic filter paper combined with immunodetection. The M13 bacteriophage was used as a model virus. A visual colour-based detection system, anti-M13 horseradish peroxidase (HRP) conjugate and 3,3',5,5'-tetramethylbenzidine (TMB), was selected to allow semi-quantitative assessment by human eye, or quantitative assessment using a digital scanner. By filtering a volume of 0.50 ml, it was possible to visually detect a concentration of 10(6) pfu/ml. The detection limit was improved to 5×10(4) pfu/ml by increasing the volume of the sample to 100ml. For comparison, it was only possible to detect a concentration of 10(7) pfu/ml using conventional sandwich enzyme-linked immunosorbent assay (ELISA) with the same detection system.


Subject(s)
Electrolytes/chemistry , Paper , Viruses/chemistry , Acrylic Resins/chemistry , Adsorption , Bacteriophage M13/chemistry , Bacteriophage M13/isolation & purification , Benzidines/chemistry , Cellulose , Electrochemistry , Enzyme-Linked Immunosorbent Assay , Filtration , Horseradish Peroxidase/chemistry , Hydrogen-Ion Concentration , Limit of Detection , Polyamines/chemistry , Polymers
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