ABSTRACT
The results of the cytogenetic monitoring of population groups that are critical with respect to the intensity of irradiation exposure (liquidators of the accident, including those who suffered from acute atomic disease) or to sensitivity of cell genome to irradiation (children living in zones with radioactive contamination) indicate a significant increase in the frequency of both chromosomal aberrations and markers of the irradiation effect in each of the groups and correlate with the ecologic situation in the regions studied.
Subject(s)
Chromosome Aberrations/genetics , Environmental Monitoring , Power Plants , Radioactive Hazard Release , Radioactive Pollutants/adverse effects , Adult , Child , Convalescence , Humans , Lymphocytes/radiation effects , Lymphocytes/ultrastructure , Occupational Exposure/adverse effects , Radiation Injuries/blood , Radiation Injuries/genetics , UkraineABSTRACT
We have examined 15 children (born in 1987-1988), whose fathers liquidated the aftereffects of the accident at Chernobyl Nuclear Power Plant and suffered from acute radiation sickness of the 1st and 2nd stages and 50 children of the control group. The obtained data showed that the number of small developmental abnormalities (stigmas of dysembryogenesis) increased as well as the chromatid aberration frequency as compared with the control group.
Subject(s)
Accidents , Chromosome Aberrations , Nuclear Reactors , Power Plants , Radiation Injuries/genetics , Abnormalities, Radiation-Induced/epidemiology , Abnormalities, Radiation-Induced/genetics , Acute Disease , Adult , Child , Child, Preschool , Chromosome Banding , Fathers , Humans , Incidence , Male , Metaphase , Ukraine/epidemiologyABSTRACT
Different methods for obtaining early mitotic chromosomes have been comparatively analyzed. Lymphocyte cultures have been treated with methotrexate, actinomycin-D and with a combination of both agents. It is shown that a high rate of chromosomes in prophase, prometaphase and mid-metaphase can be achieved by the combined methotrexate and actinomycin-D treatment of the lymphocyte culture. Methotrexate treatment of cultures is preferable to obtain the great number of early metaphase chromosomes.
