ABSTRACT
Content of fibronectin was studied in lymph and blood plasma of 24 healthy 23-57 years old men. Concentration of fibronectin in lymph and blood plasma was 44.2 +/- 21.7 mg/ml and 354 +/- 88 mg/ml, respectively. Interrelations were not found between content of fibronectin in lymph and blood plasma as well as between the total content of protein and fibronectin in lymph. Fibronectin in lymph was shown to occur in its intact form using immunoblotting procedure involving monoclonal antibodies to fibronectin. Simultaneously with whole molecules of fibronectin its fragments appear to be present in lymph.
Subject(s)
Fibronectins/analysis , Lymph/analysis , Adult , Blood Proteins/analysis , Fibronectins/blood , Humans , Immunoblotting , Male , Middle Aged , Reference ValuesABSTRACT
Over four hundred industrial workers underwent immunological examination for quantitative and functional evaluation of the main blood lymphocyte and neutrophil subpopulations and reactions of sensitization to industrial allergens at the cellular and subcellular levels. Concurrent quantitative and functional T- and B-lymphocyte deficiency was revealed in 17.5 per cent workers; the majority of subjects were found to have changes in specific indices (30 per cent) or heterogeneous changes (11.5 per cent). The incidence and magnitude of the secondary immunodeficiency signs are shown to be dependent on the main unfavourable factors of the industrial environment which are also related to the sensitization to the industrial allergens, developing primarily in T- and B-lymphocyte deficient subjects (40 per cent of the 69 per cent sensitized). A principle of group formation is proposed for the dynamic follow-up of the outcome of the revealed signs of secondary immunodeficiency and their effect on the workers' health. A methodology is established for revealing secondary immunodeficiency state and sensitization during mass immunological examinations of industrial workers.
