[Effect of estradiol dipropionate on protein synthesis in oocytes and ovary of the sea urchin Strongylocentrotus intermedius at different stages of the reproductive cycle]. / Vliianie éstradiola dipropionata na sintez belka v ootsitakh i iaichnike morskogo ezha Strongylocentrotus intermedius na razlichnykh stadiiakh reproduktivnogo tsikla.

We studied protein synthesis in the oocytes and ovary of the sea urchin Strongylocentrotus intermedius at different stages of the reproductive cycle after treatment with estradiol dipropionate. During the early development of oocytes and active gametogenesis, this estrogen induced the incorporation of 3H-leucine in the oocytes. The changes in synthetic activity of cells were accompanied by an elevated efficient incorporation of free amino acid in proteins due to its increased pool, increased tissue permeability for precursors, and increased rate of protein synthesis. Before spawning, estradiol dipropionate did not cause any changes in protein synthesis. After estradiol dipropionate treatment, the inhibitors of protein synthesis, puromycin and actinomycin D, decreased the intensity of 3H-leucine incorporation in the oocytes and protein synthesis in the ovary. The involvement of estradiol dipropionate in the regulation of protein synthesis in the sea urchin gonad is discussed.

[Localization of 17 beta-hydroxysteroid dehydrogenase in the gonads of bivalve mollusks--the sea pecten (Patinopecten yessoensis Jay) and Gray's mussel (Crenomytilus grayanus Dunker)]. / Lokalizatsiia 17 beta-gidroksisteroiddegidrogenazy v gonadakh dvustvorchatykh molliuskov--primorskogo grebeshka i midii Greia.

Presence of 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) in testes and ovaries of the common mussels--Patinopecten yessoensis (Jay) and Crenomytilus grayanus (Dunker) has been demonstrated histochemically. The enzyme is revealed in some granular amebocytes and germ cells. In growing oocytes its activity is higher that in oocytes completed their growth. 17 beta-HSD is revealed electron microscopically near agranular endoplasmic reticulum, or on the external surface of its membranes and in globules, possessing, evidently, lipid nature. The data obtained demonstrate that synthesis and metabolism of steroid hormones are possible both in additional gonadal elements and in germ cells of the animals investigated.