Effect of COL5A1, GDF5, and PPARA Genes on a Movement Screen and Neuromuscular Performance in Adolescent Team Sport Athletes.

Stastny, P, Lehnert, M, De Ste Croix, M, Petr, M, Svoboda, Z, Maixnerova, E, Varekova, R, Botek, M, Petrek, M, Lenka, K, and Cieszczyk, P. Effect of COL5A1, GDF5, and PPARA genes on a movement screen and neuromuscular performance in adolescent team sport athletes. J Strength Cond Res 33(8): 2057-2065, 2019-The risk of injury increases with adolescents' chronological age and may be related to limited muscle function neuromuscular, genetic, and biomechanical factors. The purpose of this study was to determine whether COL5A1, PPARA, and GDF5 genes are associated with muscle functions and stretch-shortening cycle performance in adolescent athletes. One hundred forty-six youth players (14.4 ± 0.2 years) from various team sports (basketball n = 54, soccer n = 50, handball n = 32) underwent a manual test for muscle function, maturity estimation, functional bend test (FBT), passive straight leg raise (SLR) test, leg stiffness test, test of reactive strength index (RSI), and gene sampling for COL5A1, PPARA, and GDF5. The χ test did not show any differences in allele or genotype frequency between participants before and after peak height velocity. Multivariate analysis of variance showed that COL5A1 rs12722 CT heterozygotes had worse score in FBT (p < 0.001), worse score in SLR (p = 0.003), and lower maturity offset (p = 0.029, only in females) than TT homozygotes. Male GDF5 rs143383 GG homozygotes showed better score in SLR than AA and AG genotypes (p = 0.003), and AA and AG genotypes in both sex had greater RSI than GG homozygotes (p = 0.016). The PPARA rs4253778 CC homozygotes had greater RSI than GG and GC genotypes (p = 0.004). The CT genotype in COL5A1 rs12722 is possible predictor of functional movement disruption in the posterior hip muscle chain, causing shortening in FBT and SLR, which includes hamstrings function. CT genotype in COL5A1 rs12722 should be involved in programs targeting hamstring and posterior hip muscle chain.

Does maturation influence neuromuscular performance and muscle damage after competitive match-play in youth male soccer players?

Poor neuromuscular control and fatigue have been proposed as a risk factor for non-contact injuries especially around peak height velocity (PHV). This study explored the effects of competitive soccer match-play on neuromuscular performance and muscle damage in male youth soccer players. 24 youth players aged 13-16y were split into a PHV group (-0.5 to 0.5y) and post PHV group (1.0-2.5y) based on maturity off-set. Leg stiffness, reactive strength index (RSI), muscle activation, creatine kinase (CK), and muscle soreness were determined pre and post a competitive soccer match. Paired t-tests were used to explore differences pre and post competitive match play and independent sample t-tests for between groups differences for all outcome measures. There was no significant fatigue-related change in absolute and relative leg stiffness or muscle activation in both groups, except for the gastrocnemius in the post PHV group. RSI, CK and perceived muscle soreness were significantly different after soccer match-play in both groups with small to large effects observed (ES:0.41-2.82). There were no significant differences between the groups pre match-play except for absolute and relative leg stiffness (P < 0.001; ES = 1.16 and 0.63 respectively). No significant differences were observed in the fatigue related responses to competitive match play between groups except for perceived muscle soreness. The influence of competitive match-play on neuromuscular function and muscle damage is similar in male youth around the time of PHV and those post-PHV indicating that other factors must contribute to the heightened injury risk around PHV.

Changes in Injury Risk Mechanisms after Soccer-Specific Fatigue in Male Youth Soccer Players.

The aim of this study was to examine the acute effects of soccer specific fatigue on muscular and neuromuscular function in male youth soccer players. Elite soccer players (n = 20; age 15.7 ± 0.5 y; body height 177.75 ± 6.61 cm; body mass 67.28 ± 8.29 kg) were measured before and after soccer specific exercise (SAFT90). The reactive strength index (RSI) was determined by a drop jump test, leg stiffness (LS) by a 20 sub-maximal two-legged hopping test, and a functional hamstring to quadriceps strength ratio from isokinetic concentric and eccentric strength of the dominant and non-dominant leg (measured at angular velocities of 1.05 rad · s-1 and 3.14 rad · s-1). Metabolic response to the SAFT90 was determined by blood lactate and perceived exertion was assessed by the Borg scale. After simulated match play, a significant decrease in absolute LS (t = 4.411; p < 0.001; ω2 = 0.48) and relative LS (t = 4.326; p < 0.001; ω2 = 0.49) was observed and the RSI increased significantly (t = 3.806; p = 0.001; ω2 = 0.40). A reduction in LS found after the SAFT90 indicates possible reduction in dynamic knee stabilization. However, if we consider the changes in other observed variables, the present study did not clearly confirm that fatigue induced by a soccer specific protocol increased the risk of ACL and hamstring injury. This may be attributed to the simulated rather than actual match play used in the present study.

Evaluation of postural asymmetry and gross joint mobility in elite female volleyball athletes.

The purpose of the study was to evaluate marked postural asymmetry and gross joint mobility in elite female volleyball athletes. Sixty-two Czech and Slovak elite female volleyball athletes (age 20.7±2.03 years, body mass 71.1±6.18 kg, body height 1.804±.0618 m, BMI 21.8±1.78) were examined by an experienced rehabilitation physician. The set of tests included the frontal posture gross examination, the forward bending test from the standing position and the deep squat test. The spiking hand and the presence of any lower extremity injury were estimated by interview. The proportion test, Mann-Whitney test and t-test were used to evaluate statistical significance (p<0.05). Fifty subjects (80.6%) exhibited "typical" frontal plane posture in which the acromion, scapula and the iliac crest were in a higher position on the left side than on the right, significantly more frequently than all the other patterns (proportion test, p<0.0001). Ninety-eight percent of the subjects with the "LLL pattern" preferred the right arm for spiking (proportion test, p<0.0001). Forty-one subjects (66%) exhibited hypermobility in the forward bending test, significantly more frequently than twenty-one subjects (34%) with normal results (proportion test, p=0.0003). Thirty-four subjects (55%) did not succeed in the deep squat test and hypermobility in the forward bending test paradoxically prevailed in them significantly (proportion test, p=0.004). Restriction in the deep squat test was not linked to obesity, age (t-test, p=0.081) nor knee (proportion test, p=0.85) and ankle injury (Mann-Whitney test, p=0.36) in the past. Significant prevalence of hypermobility in the forward bending test was not surprising because of general body composition and the performance of regular stretching exercises in elite female volleyball athletes. On the other hand, surprisingly, more than half of the subjects did not succeed in the deep squat test. The cause of poor results in the deep squat test could be due to the tightness of the soleus muscle suffering from chronic overloading and/or an inappropriate stretching methods. An inappropriate and/or insufficient compensatory exercise and stretching method or system could be the cause of their marked postural asymmetry as well. A detailed examination of posture and muscle imbalance performed by an experienced physician or physiotherapist as well as individually tailored compensatory exercises and a stretching system can be strongly recommended to all elite athletes, not only to volleyball players.

Changes in clusterin serum concentration levels in oncologic patients during the course of spa therapy--a pilot study.

BACKGROUND: Clusterin (70-80 kDa; synonym ApoJ) is a stress-associated cytoprotective glycoprotein involved in many physiological and pathophysiological processes and it is up-regulated by various apoptotic triggers in many cancers and neurodegenerative diseases. AIM: Measurement of serum clusterin values in individuals with a history of cancer before and after spa therapy. METHODS: Serum clusterin concentration (ELISA) was determined in a group of 26 oncologic patients (4 men and 22 women) at the beginning and at the end (the 18th or 19th day) of spa treatment. The spa treatment lasted 3 weeks. The patients with various types of cancer had undergone basic therapy (surgery, chemotherapy, actinotherapy) prior to spa treatment. They were divided according to the interval between the end of basic treatment and the start of spa therapy. Patients coming within 12 months comprised group A (n=15) while patients coming later comprised group B (n=11). RESULTS: clusterin concentrations increased in 11 patients (73%) and decreased in 4 (27%) in group A and increased in 5 (45%) and decreased in 6 (55%) in group B. The non-parametric sign test was non-significant. There were positive value of average change between the second and the first sample in group A and negative value in group B. In group A the parametric test showed significant increased clusterin concentration at the end of spa treatment but the data had non-parametric distribution in fact. CONCLUSION: It is concluded that early spa therapy increases clusterin serum concentration. This is probably due to to the positive effects of balneotherapy. However the sample was very small and further research is required.