Global decrease in heavy metal concentrations in brown algae in the last 90 years.

In the current scenario of global change, heavy metal pollution is of major concern because of its associated toxic effects and the persistence of these pollutants in the environment. This study is the first to evaluate the changes in heavy metal concentrations worldwide in brown algae over the last 90 years (>15,700 data across the globe reported from 1933 to 2020). The study findings revealed significant decreases in the concentrations of Cd, Co, Cr, Cu, Fe, Hg, Mn, Pb and Zn of around 60-84% (ca. 2% annual) in brown algae tissues. The decreases were consistent across the different families considered (Dictyotaceae, Fucaceae, Laminariaceae, Sargassaceae and Others), and began between 1970 and 1990. In addition, strong relationships between these trends and pH, SST and heat content were detected. Although the observed metal declines could be partially explained by these strong correlations, or by adaptions in the algae, other evidences suggest an actual reduction in metal concentrations in oceans because of the implementation of environmental policies. In any case, this study shows a reduction in metal concentrations in brown algae over the last 50 years, which is important in itself, as brown algae form the basis of many marine food webs and are therefore potential distributors of pollutants.

Can proteomics contribute to biomonitoring of aquatic pollution? A critical review.

Aquatic pollution is one of the greatest environmental problems, and therefore its control represents one of the major challenges in this century. In recent years, proteomics has emerged as a powerful tool for searching protein biomarkers in the field of pollution biomonitoring. For biomonitoring marine contamination, there is a consensus that bivalves are preferred organisms to assess organic and inorganic pollutants. Thus, the bivalve proteome was intensively studied, particularly the mussel. It is well documented that heavy metal pollution and organic chemicals altered the structural proteins causing degradation of tissues of molluscs. Also, it is well known that proteins involved in stress oxidative such as glutathione and enzymes as catalase, superoxide dismutase or peroxisomes are overexpressed in response to contaminants. Additionally, using bivalves, other groups of proteins proposed as pollution biomarkers are the metabolic proteins. Even though other marine species are used to monitor the pollution, the presence of proteomic tools in these studies is scarce. Concerning freshwater pollution field, a great variety of animal species (fish and crustaceans) are used as biomonitors in proteomics studies compared to plants that are scarcely analysed. In fish species, proteins involved in stress oxidative such as heat shock family or proteins from lipid and carbohydrate metabolism were proposed as candidate biomarkers. On the contrary, for crustaceans there is a lack of proteomic studies individually assessing the contaminants. Novel scenarios, including emerging contaminants and new threats, will require proteomic technology for a systematic search of protein biomarkers and a greater knowledge at molecular level of those cellular pathways induced by contamination.

Sampling optimization for biomonitoring metal contamination with marine macroalgae.

The aim of the present study was to optimize the protocol for sampling marine macroalgae to be used to biomonitor heavy metal contamination in marine ecosystems. For this purpose, we collected 50 subsamples of the brown seaweed Fucus vesiculosus at random in each of three sampling sites (SS) and determined the concentrations of Al, As, Cd, Co, Cr, Cu, Fe, Hg, N, Ni, Pb, Zn and δ15N. We used semivariograms to explore the possible existence of spatial structure in the concentrations of the elements. Spatial structure was observed in 88% of the semivariograms studied, with element concentrations varying longitudinally and transversally along the SS. Using randomization techniques, we estimated that in each SS, a minimum of 30 evenly distributed subsamples should be collected within three bands parallel to the coastline (and also at different heights on the rocks if necessary), and analyzed in a single composite sample representative of the intra-SS variability.

Evaluation of the use of moss transplants (Pseudoscleropodium purum) for biomonitoring different forms of air pollutant nitrogen compounds.

We investigated whether three different types of moss transplants (devitalized moss bags with and without cover and auto-irrigated moss transplants) are suitable for use as biomonitors of the deposition of oxidised and/or reduced forms of N. For this purpose, we determined whether the concentration of atmospheric NO2 was related to the % N, δ(15)N and the activity of the enzyme biomarkers phosphomonoesterase (PME) and nitrate reductase (NR) in the tissues of moss transplants. We exposed the transplants in 5 different environments of Galicia (NW Spain) and Cataluña (NE Spain): industrial environments, urban and periurban environments, the surroundings of a cattle farm and in a monitoring site included in the sampling network of the European Monitoring Programme. The results showed that the moss in the auto-irrigated transplants was able of incorporating the N in its tissues because it was metabolically active, whereas in devitalized moss bags transplants, moss simply intercepts physically the N compounds that reached it in particulate or gaseous form. In addition, this devitalization could limit the capacity of moss to capture gaseous compounds (i.e. reduced N) and to reduce the oxidised compounds that reach the specimens. These findings indicate that devitalized moss transplants cannot be used to monitor either oxidised or reduced N compounds, whereas transplants of metabolically active moss can be used for this purpose. Finally, the NR and PME biomarkers should be used with caution because of the high variability in their activities and the limits of quantification should be evaluated in each case.

Study of temporal trends in mercury concentrations in the primary flight feathers of Strix aluco.

Temporal trends in Hg concentrations were determined in the primary flight feathers of 146 specimens of Strix aluco which had died in various Wildlife Recovery Centres in Galicia (NW Spain) between 1997 and 2014. The aim of the study was to determine whether standardization of a primary flight feather (or feathers) in this species is essential for identifying temporal trends in Hg concentrations. For this purpose, we had to first standardize the feather(s) analyzed to enable comparison of the levels of Hg detected in different feathers. The results show a high degree of both inter and intra-individual variability but despite that, it was possible to identify P5 as the most representative feather taking into account the amount of metal excreted in each feather and the intra-individual variability: its median was 133ng, which represents 15% (from 7% to 15%) of the total Hg present in all the primary feathers. However, this "standard feather" did not reveal any temporal trend in Hg concentrations for the study period. This lack of trend was found irrespective of the feather considered and it is expected that detection of any existing trend would also not depend on the feather considered. We conclude that use of any particular feather is not essential for identifying temporal trends in Hg concentrations, because the pattern will be identified regardless of the feather selected.

Best options for the exposure of traditional and innovative moss bags: A systematic evaluation in three European countries.

To develop an internationally standardized protocol for the moss bag technique application, the research team participating in the FP7 European project "MOSSclone" focused on the optimization of the moss bags exposure in terms of bag characteristics (shape of the bags, mesh size, weight/surface ratio), duration and height of exposure by comparing traditional moss bags to a new concept bag, "Mossphere". In particular, the effects of each variable on the metal uptake from the air were evaluated by a systematic experimental design carried out in urban, industrial, agricultural and background areas of three European countries with oceanic, Mediterranean and continental climate. The results evidenced that the shape, the mesh size of the bags and the exposure height (in the tested ranges), did not significantly influence the uptake capacity of the transplanted moss. The aspects more affecting the element uptake were represented by the density of the moss inside the bags and the relative ratio between its weight and the surface area of the bag. We found that, the lower the density, the higher the uptake recorded. Moreover, three weeks of exposure were not enough to have a consistent uptake signal in all the environments tested, thus we suggest an exposure period not shorter than 6 weeks, which is appropriate in most situations. The above results were confirmed in all the countries and scenarios tested. The adoption of a shared exposure protocol by the research community is strongly recommended since it is a key aspect to make biomonitoring surveys directly comparable, also in view of its recognition as a monitoring method by the EU legislation.

Active biomonitoring with the moss Pseudoscleropodium purum: Comparison between different types of transplants and bulk deposition.

Active biomonitoring with terrestrial mosses can be used to complement traditional air pollution monitoring techniques. Several studies have been carried out to compare the uptake capacity of different types of moss transplants. However, until now the relationship between the uptake of elements in devitalized moss bags and in irrigated transplants has not been explored. In this study, the final concentrations of Cd, Cu, Hg, Pb and Zn were determined in irrigated and devitalized moss transplants in the surroundings of a steelworks. The concentrations were also compared with those of the same elements in the bulk deposition to determine which type of moss transplant yields the closest correlations. Devitalized moss retained higher concentrations of all of the elements (except Hg) than the irrigated moss. Both irrigated and devitalized moss transplants appear to detect the same type of contamination (i.e. particulate matter and dissolved metals rather than gaseous forms) as significant correlations were found for Cu, Hg, Pb and Zn, whereas, neither type of the moss transplant was sensitive enough to detect changes in the soluble fraction load of bulk deposition. Further studies will be needed to a better understanding of the correlation between the concentrations of elements in moss transplants with the particulate fraction of the bulk deposition. This will enable the establishment of a more robust and accurate biomonitoring tool.

Use of a moss biomonitoring method to compile emission inventories for small-scale industries.

We used a method of detecting small-scale pollution sources (DSSP) that involves measurement of the concentrations of elements in moss tissues, with the following aims: (i) to determine any common qualitative patterns of contaminant emissions for individual industrial sectors, (ii) to compare any such patterns with previously described patterns, and (iii) to compile an inventory of the metals and metalloids emitted by the industries considered. Cluster analysis revealed that there were no common patterns of emission associated with the industrial sectors, probably because of differences in production processes and in the types of fuel and raw materials. However, when these variables were shared by different factories, the concentrations of the elements in moss tissues enabled the factories to be grouped according to their emissions. We compiled a list of the metals and metalloids emitted by the factories under study and found that the DSSP method was satisfactory for this purpose in most cases (53 of 56). The method appears to be a useful tool for compiling contaminant inventories; it may also be useful for determining the efficacy of technical improvements aimed at reducing the industrial emission of contaminants and could be incorporated in environmental monitoring and control programmes.

Establishing reference values of spectral reflectance indices in transplants of Pseudoscleropodium purum for potential use in atmospheric biomonitoring.

We studied the spatiotemporal variation of the photochemical reflectance index (PRI) and the chlorophyll content index (CHL index) in a terrestrial moss, using self-watering transplants distributed in 8 sampling sites that were sampled periodically during up to 4 years. We did not detect any seasonal patterns or difference between the studied sites, and therefore the variation across the sites reflected the influence of environmental variables in the area. We established the reference thresholds associated with physiological stress as -0.212 for PRI and 1.195 for CHL index. Our findings could be applied for biomonitoring atmospheric contamination on the basis of the physiological stress shown by the moss used.

On the use of epigaeic mosses to biomonitor atmospheric deposition of nitrogen.

In this study, we investigated whether the terrestrial moss Pseudoscleropodium purum can be used to biomonitor atmospheric deposition of nitrogen (N). For this purpose, we first determined whether there are any interspecific differences in the concentrations of total N and δ(15)N between the two species of terrestrial moss most commonly used in biomonitoring studies, P. purum and Hypnum cupressiforme. Second, we determined the spatial distribution of N and δ(15)N at small and large scales: (1) by analysis of 165 samples from the surroundings of an aluminium smelter and (2) by analysis of 149 samples from sites forming part of a regular 15 × 15-km sampling network in Galicia (northwest Spain). We did not find any interspecific differences in either total N or δ(15)N. Analysis of δ(15)N enabled us to identify large-scale spatial patterns of distribution that were congruent with the location of the main N emission sources (unlike the analysis of total N). However, we did not identify any such patterns for the small-scale source of N emission studied. The results show that analysis of δ(15)N has an advantage compared with the analysis of total N in that it provides information about the source of N rather than about the amount of N received. Furthermore, isotope discrimination appears to occur, with the bryophytes preferentially accumulating the N(14) isotope. Although this amplifies the signal of reduced forms, it is not problematical for determining spatial-distribution patterns.

[Living a socio-cultural approach in nursing education]. / Vivenciando uma abordagem sócio-cultural no ensino de enfermagem.

The paper describes a teaching experience linking an undergraduate course and Master's degree course strictu sensu. The aim is to reflect on the social-cultural approach in nursing teaching and the relationship between teacher and students in the classroom. The subject of the study were students and a teacher from the discipline Philosophical Anthropology (graduate course) and student of the discipline Methodology of nursing teaching (master's course). The methodology applied was varied. The master's student followed the discipline through a participant observation and taught eight hours of class. On the participant observation of classes Paulo Freire's concepts of relationship between teacher and student and teaching-learning were applied. The feedback from the subjects in relation to the experience was positive.

[Brazilian Nursing Association: 70 years of struggle for life]. / Associação Brasileira de enfermagem: 70 anos de luta pela vida.

This work is about the Brazilian Nursing Association (ABEn), its origin, its expansion and projection based on its philosophy and highlighting its achievements from its origin in 1926 until today when its 70th anniversary is being celebrated. This work aims at reporting this institution history and narrating the support provided so that nursing may contribute decisively to the construction of a body of profession knowledge itself, the legal definition of professional practice, teaching regulamentation through its commissions. Methodology has been composed of bibliographic research and interviews with active professionals. It has been concluded that ABEn is stronger and stronger and aware of the way on how to achieve many other goals which will bear great conquests which will contribute for granting nursing power and space as a profession set to the human, therefore, to a better life, with more love, peace and social justice.