ABSTRACT
Colorectal cancer mortality rate and highly altered proteins from the Wnt/ß-catenin pathway increase the scientific community's interest in finding alternatives for prevention and treatment. This study aims to determine the biological effect of chlorogenic acid (CGA) on two colorectal cancer cell lines, HT-29 and SW480, and its interactions with ß-catenin and LRP6 to elucidate a possible modulatory mechanism on the Wnt/ß-catenin pathway. These effects were determined by propidium iodide and DiOC6 for mitochondrial membrane permeability, MitoTracker Red for mitochondrial ROS production, DNA content for cell distribution on cell cycle phases, and molecular docking for protein-ligand interactions and binding affinity. Here, it was found that CGA at 2000 µM significantly affects cell viability and causes DNA fragmentation in SW480 cells rather than in HT-29 cells, but in both cell lines, it induces ROS production. Additionally, CGA has similar affinity and interactions for LRP6 as niclosamide but has a higher affinity for both ß-catenin sites than C2 and iCRT14. These results suggest a possible modulatory role of CGA over the Wnt/ß-catenin pathway in colorectal cancer.
ABSTRACT
La púrpura de Henoch Schönlein es una vasculitis sistémica que afecta vasos pequeños, predominantemente en niños. Los eventos trombóticos son una rara complicación, y en la literatura solo algunos casos se han reportado. Entre éstos, un caso de oclusión de arteria central de la retina bilateral y un caso de oclusión de vena central de la retina, ambos pacientes con diagnóstico de púrpura de Henoch Schönlein, el segundo dos semanas después a iniciar hemodiálisis. Describimos el caso de un paciente adulto con diagnóstico de púrpura de Henoch Schönlein e hipertensión arterial, en insuficiencia renal crónica estadio V, en hemodiálisis hacía 20 días, quien se presentó con disminución de agudeza visual de 15 días de evolución en ojo derecho. Los hallazgos al examen ocular fueron edema del disco, edema macular, tortuosidad vascular, hemorragias en cuatro cuadrantes, se consideró una oclusión de vena central de la retina. Requirió terapia antiangiogénica y fotocoagulación laser con buena evolución.
Henoch Schönlein purpura is a systemic vasculitis that affects small vessels and mainly aff ecting children. Thrombotic events are a rare complication, and only a few cases have been reported. Among these a case of bilateral central retinal arterial occlusion in patients with Henoch Schönlein purpura, and a case of central retinal vein occlusion in a patient with Henoch Schönlein purpura over a two weeks period of hemodialysis. We describe the case of an adult patient diagnosed with Henoch Schönlein purpura and arterial hypertension, chronic renal failure in hemodialysis for 20 days; who presents with decreased visual acuity for 15 days in the right eye. The findings were disc edema, macular edema, vascular tortuosity, haemorrhages in four quadrants. Central retinal vein occlusion was considered and antiangiogenic therapy and laser photocoagulation was performed with good response.
