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1.
Cuad. Hosp. Clín ; 60(2): 32-38, dic. 2019. ilus.
Article in Spanish | LILACS | ID: biblio-1046719

ABSTRACT

INTRODUCCIÓN: la cátedra de pediatría con apoyo del Instituto de Investigación en Salud y Desarrollo de la UMSA, ha elaborado un módulo de enseñanza teórico práctico para brindar conocimiento científico actualizado sobre alimentación y nutrición del menor de dos años. Durante la última gestión universitaria se ha incursionado en la enseñanza virtual para incrementar el tiempo de lectura, y contar con mayor espacio para las actividades prácticas. MÁTERIALES Y MÉTODOS: el objetivo de este artículo es identificar las fortalezas y debilidades del proceso de enseñanza y aprendizaje (PEA) combinado; usando metodología cuantitativa (pre y post-prueba) y cualitativa (grupo focal). Se contó con un total de 39 estudiantes. La prueba de conocimientos post intervención tuvo un puntaje de 771 mayor que la previa (p=0,000 ((IC 95% 642,35 - 901,23); y se respondió en menor tiempo (-219,43 segundos p=0.0000 (IC 95% (146,98 ­ 291,88). Un 81% de estudiantes estaba en el límite o por encima de lo que se consideró mínimo adecuado de facilidades informáticas. El grupo focal evidencio dificultades en el portal virtual, como muchos pasos para el ingreso, la saturación del servicio y otras; se dieron múltiples sugerencias para mejorar el módulo. RESULTADOS: este estudio demuestra que además de adquirir conocimientos, los estudiantes se han involucrado en los diferentes aspectos PEA; en base a sus sugerencias se ha comenzado a realizar cambios incluyendo la introducción de herramientas digitales de uso colaborativo como Wiki. CONCLUSIÓN: se concluye que el PEA combinado aplicado en la catedra de pediatría de la UMSA mejora la adquisición de conocimientos y prácticas sobre lactancia materna y alimentación complementaria del menor de dos años.


INTRODUCTION: the pediatric department with the support of the Institute of Research in Health and Development of the UMSA has developed a practical and theoretical teaching module to provide updated scientific knowledge on food and nutrition for children under two years of age. During the last university year, virtual teaching has been introduced to increase reading time, and to have more space for practical activities. MATERIAL AND METHODS: the objective of this article is to identify the strengths and weaknesses of the combined Learning Teaching Process using quantitative (pre and post-test) and qualitative methodology (focus group); 39 students participated in the study. In the post-intervention knowledge test obtained a score of 771 higher than the one prior to intervention (p = 0.000 ((95% CI 642.35 - 901.23), and spent shorter time in answers (-219.43 seconds p = 0.0000 (95% CI % (146.98 - 291.88). A total of 81% of students were in the limit or above what was considered adequate or minimum computer facilities. The focus group evidenced difficulties in the virtual portal, as many steps for the entrance, the saturation of the service and others, multiple suggestions were given to improve the module. RESULTS: this study shows that in addition to acquiring knowledge, students have been involved in the different aspects of the Teaching Learning Process (TLP). Based on their suggestions, changes such as the introduction of collaborative digital tools (Wiki) have been made. CONCLUSIONS: the article concludes indicating that the combined TLP applied in the pediatric department of the UMSA improves the students' acquisition of knowledge and practices on breastfeeding and complementary feeding of the child two years.


Subject(s)
Teaching/education , Universities , Learning
2.
Poult Sci ; 91(12): 3148-55, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23155025

ABSTRACT

This work studied the effect of supplementing commercially available amino acids in low-protein diets using different ratios of digestible (dig) glycine+serine:lysine (Gly+Ser:Lys) on performance, serum parameters, feathering, and litter characteristics of broiler chickens during the starter period. A total of one thousand fifty 1-d-old Cobb-Vantress male chicks were distributed in a completely randomized experimental design into 6 treatments with 5 replicates of 35 birds each. The treatments were as follows: T1, control diet based on corn and soybean meal formulated with 22% CP (dig Gly+Ser:Lys ratio of 147); T2, diet with a 2% CP reduction, supplemented with Val (dig Gly+Ser:Lys ratio of 137); T3, similar to T2 with the addition of Gly (dig Gly+Ser:Lys ratio of 147); T4, diet with a 3% CP reduction, supplemented with Val, Ile, and Arg (dig Gly+Ser:Lys ratio of 127); and T5 and T6, similar to T4 with the addition of Gly (dig Gly+Ser:Lys ratios of 137 and 147, respectively). At 7 and 21 d, broilers that had received diets with a 3% CP reduction (19% CP) and a Gly+Ser:Lys ratio that was equivalent to 127 had lower G:F (P < 0.05) and lower total protein and albumin serum concentrations (P < 0.05) than those broilers that received the control feed. However, these parameters were restored to the same level as the control diet with an increase in the dig Gly+Ser:Lys ratio from 127 to 137 and 147. Diets with a 3% CP reduction (19% CP) resulted in litter with reduced (P < 0.05) nitrogen content and lower ammonia emission than the litter of broilers receiving the control diet. The treatments did not influence (P > 0.05) the feather length or feathering scores at 21 or 28 d of age. The supplementation of essential amino acids while maintaining dig Gly+Ser:Lys ratios at and above 137 allowed for a reduction in the dietary CP of 3% without undermining the performance, feathering or serum parameters of early stage broilers.


Subject(s)
Animal Feed/analysis , Chickens , Dietary Proteins/administration & dosage , Glycine/pharmacology , Lysine/pharmacology , Serine/pharmacology , Animal Nutritional Physiological Phenomena , Animals , Chickens/growth & development , Diet/veterinary , Dietary Supplements , Glycine/administration & dosage , Glycine/chemistry , Lysine/administration & dosage , Lysine/chemistry , Male , Serine/administration & dosage , Serine/chemistry
3.
Braz. j. med. biol. res ; 40(10): 1399-1402, Oct. 2007. graf
Article in English | LILACS | ID: lil-461367

ABSTRACT

The cytotoxic activity of amino (3a-e), aza-1-antraquinone (4a-e) lapachol derivatives against Ehrlich carcinoma and human K562 leukemia cells was investigated. Cell viability was determined using MTT assay, after 48 (Ehrlich) or 96 h (K562) of culture, and vincristine (for K562 leukemia) and quercetin (for Ehrlich carcinoma) were used as positive controls. The results showed dose-dependent growth-inhibiting activities and that the amino derivatives were active against the assayed cells, whereas the 4a-e derivatives were not. The allylamine derivative 3a was the most active against Ehrlich carcinoma, with IC50 = 16.94 ± 1.25 muM, and against K562 leukemia, with IC50 = 14.11 ± 1.39 muM. The analogous lawsone derivative, 5a, was also active against Ehrlich carcinoma (IC50 = 23.89 ± 2.3 muM), although the 5d and 5e derivatives showed lower activity. The interaction between 3a-d and calf thymus DNA was investigated by fluorimetric titration and the results showed a hyperchromic effect indicating binding to DNA as presented of ethidium bromide, used as positive control. The inhibitory action on DNA-topoisomerase II-a was also evaluated by a relaxation assay of supercoiled DNA plasmid, and the etoposide (200 muM) was used as positive control. Significant inhibitory activities were observed for 3a-d at 200 muM and a partial inhibitory action was observed for lapachol and methoxylapachol.


Subject(s)
Animals , Humans , Mice , Antineoplastic Agents, Phytogenic/pharmacology , Carcinoma, Ehrlich Tumor/enzymology , DNA Topoisomerases, Type II/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Naphthoquinones/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antioxidants/pharmacology , Cell Survival/drug effects , Enzyme Inhibitors/chemistry , /drug effects , Naphthoquinones/chemistry , Quercetin/pharmacology , Vincristine/pharmacology
4.
Braz J Med Biol Res ; 40(10): 1399-402, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17713652

ABSTRACT

The cytotoxic activity of amino (3a-e), aza-1-antraquinone (4a-e) lapachol derivatives against Ehrlich carcinoma and human K562 leukemia cells was investigated. Cell viability was determined using MTT assay, after 48 (Ehrlich) or 96 h (K562) of culture, and vincristine (for K562 leukemia) and quercetin (for Ehrlich carcinoma) were used as positive controls. The results showed dose-dependent growth-inhibiting activities and that the amino derivatives were active against the assayed cells, whereas the 4a-e derivatives were not. The allylamine derivative 3a was the most active against Ehrlich carcinoma, with IC50 = 16.94 +/- 1.25 microM, and against K562 leukemia, with IC50 = 14.11 +/- 1.39 microM. The analogous lawsone derivative, 5a, was also active against Ehrlich carcinoma (IC50 = 23.89 +/- 2.3 microM), although the 5d and 5e derivatives showed lower activity. The interaction between 3a-d and calf thymus DNA was investigated by fluorimetric titration and the results showed a hyperchromic effect indicating binding to DNA as presented of ethidium bromide, used as positive control. The inhibitory action on DNA-topoisomerase II-a was also evaluated by a relaxation assay of supercoiled DNA plasmid, and the etoposide (200 microM) was used as positive control. Significant inhibitory activities were observed for 3a-d at 200 microM and a partial inhibitory action was observed for lapachol and methoxylapachol.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Carcinoma, Ehrlich Tumor/enzymology , Enzyme Inhibitors/pharmacology , Naphthoquinones/pharmacology , Topoisomerase II Inhibitors , Animals , Antineoplastic Agents, Phytogenic/chemistry , Antioxidants/pharmacology , Cell Survival/drug effects , Enzyme Inhibitors/chemistry , Humans , Inhibitory Concentration 50 , K562 Cells/drug effects , Mice , Naphthoquinones/chemistry , Quercetin/pharmacology , Vincristine/pharmacology
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