ABSTRACT
OBJECTIVE: To explore biomolecular characteristics of a group of patients with nasopharyngeal carcinoma from European (Spanish) hospitals, addressing the pathogenesis of the tumor and the response to treatment. STUDY DESIGN: Cyclin D1 and p16 expression were evaluated immunohistochemically in 33 tissue samples of nasopharyngeal carcinoma. CCDN1 gene amplification and p16 gene deletion were studied by fluorescence in situ hybridization. Patient clinical data were examined, and tissues were evaluated histologically using hematoxylin-eosin staining. RESULTS: Cyclin D1 overexpression was found in 19 cases, and p16 expression was undetected in 30 cases. An association was observed between impaired p16 expression and cyclin D1 overexpression (p = 0.034). Eleven patients displayed p16 gene deletion and CCDN1 gene amplification. CONCLUSION: Cyclin D1 overexpression and CCDN1 amplification, loss of p16 expression and p16 deletion may be among the genetic alterations involved in the pathogenesis of nasopharyngeal carcinoma.
Subject(s)
Cyclin D1/genetics , Cyclin-Dependent Kinase Inhibitor p16/genetics , Nasopharyngeal Neoplasms/genetics , Adult , Aged , Cyclin-Dependent Kinase Inhibitor p16/metabolism , Female , Humans , Immunohistochemistry , In Situ Hybridization, Fluorescence , Male , Middle Aged , Nasopharyngeal Neoplasms/diagnosis , Retrospective Studies , SpainABSTRACT
Las descripciones del cuadro citológico del tumor fibrososolitario pulmonar (TFSP) en la literatura son escasas.Aportamos un caso de TFSP cuyas características de malignidadhan sido confirmadas mediante estudio histológico,inmunohistoquímico, citogenético y FISH. La presencia decélulas dendríticas es un dato morfológico orientativo en eldiagnóstico de esta lesión. No hemos confirmado en nuestrocaso la presencia de hendiduras ni pseudoinclusionesnucleares, referidas por otros autores como hallazgo frecuenteen casos de TFSP maligno(AU)
Descriptions of the cytology of pleural malignant solitaryfibrous tumour are uncommon in the literature. Wereport a case of PSFT with malignant cytological featuresconfirmed by histology, immunochemistry, citogenetics andFISH. The presence of dendritic cells is a useful diagnosticfeature. However, we were unable to confirm the presenceof nuclear clefts and pseudoinclusions reported as commonfindings by other authors(AU)
Subject(s)
Humans , Male , Female , Cytological Techniques/instrumentation , Cytological Techniques/methods , Neoplasms, Fibrous Tissue/diagnosis , Neoplasms, Fibrous Tissue/pathology , Immunohistochemistry/instrumentation , Immunohistochemistry/methods , Cytogenetics/methods , Cytogenetics/trends , Cytogenetic Analysis/trends , Dendritic Cells/pathologyABSTRACT
Los liposarcomas constituyen el grupo más numeroso de sarcomas del adulto. Su interés actual radica fundamentalmente en los cambios conceptuales y clasificatorios que han acontecido en los últimos años merced a la aplicación de las técnicas de citogenética y de biología molecular. En la presente revisión se lleva a cabo una correlación clínico patológica de los cinco tipos básicos de liposarcomas y se comentan los aspectos citogenéticos y de biología molecular que han permitido la elaboración de la nueva clasificación de la OMS
Liposarcomas are the most common sarcoma of the adult life. Their current interest is based on the recent molecular and cytogenetic changes that have allowed the new WHO classification. In the present report we carried out a clinicopathological correlation in the five distinctive groups of the current classification and described the most relevant cytogenetics and molecular findings in each group
