ABSTRACT
Changes in red blood cell (RBC) lipid peroxidation [measured by malonyl dialdehyde (MDA) concentration], glutathione (GSH) metabolism, antioxidant enzyme activities (catalase, superoxide dismutase, glutathione peroxidase) and haemoglobin (Hb) metabolites (metHb, carboxy Hb) were studied in six children with post-enteropathic (D+) haemolytic uraemic syndrome (HUS) and ten controls. The in vitro effect of hydrogen peroxide [acetylphenylhydrazine (APH) test] on GSH and Hb metabolism was also investigated. MDA levels were significantly higher and the antioxidant enzyme activities were lower in HUS patients than in the controls (P < 0.01). The oxidised glutathione concentration was significantly higher in the patients than in the control children (26.3 +/- 12.6 vs. 10.9 +/- 1.8 nmol/g Hb. Percentage values of carboxy Hb and metHb were also higher in HUS (P < 0.01). Incubation of RBC with APH induced a more pronounced decrease in the concentration of GSH (P < 0.001) and a significant increase (P < 0.01) in the level of metHb and carboxy Hb in the HUS patients. This suggests that there is reduced RBC GSH stability in HUS. Utilisation of GSH and antioxidant enzymes leads to increased Hb oxidation and haemolysis. The oxidative damage may have an important role in the pathogenesis of haemolytic anaemia in HUS.
Subject(s)
Erythrocytes/metabolism , Hemoglobins/metabolism , Hemolytic-Uremic Syndrome/blood , Lipid Peroxidation , Adolescent , Child , Child, Preschool , Female , Glutathione/blood , Humans , Infant , Male , Oxidoreductases/bloodABSTRACT
Changes in erythrocyte lipid peroxidation (measured as the concentration of malonyl dialdehyde), glutathione metabolism, antioxidant enzyme activities (glutathione peroxidase, catalase, and superoxide dismutase), the oxidized products of haemoglobin (Hb), and hydrogen peroxide (H2O2)-induced haemolysis were studied in six children with chronic renal failure treated with serial acetate and bicarbonate haemodialysis (HD). Ten age- and sex-matched children acted as controls. Malonyl dialdehyde levels were significantly higher and antioxidant enzyme activities lower in uraemic red blood cells (RBCs) compared with controls (P less than 0.05). Incubation of RBCs for 1 h with acetylphenylhydrazine induced a decrease in the concentration of reduced glutathione (P less than 0.001) and an increase in the level of oxidized products of Hb (P less than 0.001), but only in the uraemic patients. The H2O2 haemolysis test revealed a mild (n = 3) to increased (n = 3) haemolysis in the uraemic RBCs. Oxidative haemolysis is probably a multifactorial process in uraemic patients, and may be an important risk factor in HD therapy.
